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70236 | BL21(DE3)pLysS Singles™ Competent Cells - Novagen

70236
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      Overview

      Replacement Information

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      70236-3
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          Glass bottle 11 rxn
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          70236-4
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              Glass bottle 22 rxn
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              Description
              OverviewBL21 strains are the most widely used hosts for protein expression from pET recombinants and have the advantage of being deficient in both lon and ompT proteases.

              DE3 indicates that the host is a lysogen of λDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter. Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG.

              pLysS strains express T7 lysozyme, which further suppresses basal expression of T7 RNA polymerase prior to induction, thus stabilizing pET recombinants encoding target proteins that affect cell growth and viability.
              Genotype: F- ompT hsdSB(rB- mB-) gal dcm (DE3) pLysS (CamR)
              Catalogue Number70236
              Brand Family Novagen®
              References
              Product Information
              Components
              Guaranteed efficiencyGuaranteed efficiency: > 2 x 10⁷ cfu/µg
              Applications
              Biological Information
              Physicochemical Information
              Dimensions
              Materials Information
              Toxicological Information
              Safety Information according to GHS
              Safety Information
              Product Usage Statements
              Storage and Shipping Information
              Ship Code Dry Ice Only
              Toxicity Multiple Toxicity Values, refer to MSDS
              Storage ≤ -70°C
              Avoid freeze/thaw Avoid freeze/thaw
              Do not freeze Ok to freeze
              Packaging Information
              Transport Information
              Supplemental Information
              Specifications

              Documentation

              SDS

              Title

              Safety Data Sheet (SDS) 

              Certificates of Analysis

              TitleLot Number
              70236

              Citations

              Title
            • Laurent R Chiarelli, et al. (2005) Functional analysis of pyrimidine 5'-nucleotidase mutants causing nonspherocytic hemolytic anemia. Blood 105, 3340-3345.
            • Sung Hoon Kim, et al. (2005) A proteomic microarray approach for exploring ligand-initiated nuclear hormone receptor pharmacology, receptor-selectivity,and heterodimer functionality. Molecular and Cellular Proteomics 4, 267-277.
            • Subhradip Mukhopadhyay, et al. (2005) Identification of a region of fast skeletal troponin T required for stabilization of the coiled-coil formation with troponin I. Journal of Biological Chemistry 280, 538-547.
            • Martin Sprick, et al. (2004) cFLIPL inhibits tumor necrosis factor-related apoptosis-inducing ligand-mediated NF-κB activation at the death-inducing signaling complex in human keratinocytes. Journal of Biological Chemistry 279, 52824-52834.
            • User Protocols

              Title
              TB055 pET System Manual

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              Categories

              Life Science Research > Genomic Analysis > Transfection and Protein Expression > Bacterial Expression > Competent Cells for Expression