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69450 | BL21(DE3) Competent Cells - Novagen

69450
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      Overview

      Replacement Information

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      69450-3
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          Glass bottle 0.4 ml
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          69450-4
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              Glass bottle 1 ml
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              Description
              OverviewA common method for transformation of DNA plasmids into E. coli is the use of chemically competent cells. Although competent cells can be prepared in the laboratory, greater efficiency, reproducibility, and convenience are achieved using Novagen prepared competent cells. Novagen competent cells represent the widest selection available for protein expression. Every Novagen competent cell strain is verified for phenotype and purity, and is guaranteed for transformation efficiency.
              T7 expression strains are lysogens of bacteriophage DE3, as indicated by the (DE3). These hosts carry a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter. Such strains are suitable for production of protein from target genes cloned in appropriate T7 expression vectors, using IPTG as an inducer.
              BL21 has been the gold standard for protein expression since it was first introduced in 1990. Deficient in lon and ompT proteases, BL21 and its derivatives are ideal for many applications.
              Catalogue Number69450
              Brand Family Novagen®
              References
              Product Information
              Components
              Guaranteed efficiency> 2 x 10⁷ cfu/μg
              Applications
              Biological Information
              Physicochemical Information
              Dimensions
              Materials Information
              Toxicological Information
              Safety Information according to GHS
              Safety Information
              Product Usage Statements
              Storage and Shipping Information
              Ship Code Dry Ice Only
              Toxicity Multiple Toxicity Values, refer to MSDS
              Storage ≤ -70°C
              Avoid freeze/thaw Avoid freeze/thaw
              Do not freeze Ok to freeze
              Licence Brookhaven This product is covered under license from Brookhaven National Labs. Commercial entities need to obtain a research use license prior to purchase. Please contact your licensing department to confirm that your company already holds a research use license.
              Packaging Information
              Transport Information
              Supplemental Information
              Specifications

              Documentation

              SDS

              Title

              Safety Data Sheet (SDS) 

              Certificates of Analysis

              TitleLot Number
              69450

              Brochure

              Title
              Competent Cell Brochure

              Citations

              Title
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            • Qi, M., et al. 2007. Applied and Enviornmental Microbiology 73, 6098.
            • Shchedrina, V.A., et al. 2007. Procedings of the National Academy of Science 104, 13919.
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            • Park, S.-A., et al. 2006. Procedings of the National Academy of Science 103, 11886.
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            • Stewart, J.J., et al. 2006. Journal of Biological Chemistry 281, 22665.
            • Alphey, M.S., et al. 2005. Journal of Biological Chemistry 280, 3068.
            • Christophe Anjard and William F. Loomis. 2005. Procedings of the National Academy of Science 102, 7607.
            • Bachler, C., et al. 2005. European Molecular Biology Organization Journal 24, 283.
            • Banki, M.,R., et al. 2005. Protein Science 14, 1387.
            • Bausch, S.L., et al. 2005. Journal of Biological Chemistry 280, 29956.
            • Nishiwaki, H., et al. 2007. Applied and Enviornmental Microbiology 73, 3404-.
            • MichelOtte, M.M., et al. 2007. Journal of Bacteriology 189, 7310.
            • Park, M.-O., et al. 2007. Journal of Bacteriology 189, 7281.
            • Qi, M., et al. 2007. Applied and Enviornmental Microbiology 73, 6098.
            • Shchedrina, V.A., et al. 2007. Procedings of the National Academy of Science 104, 13919.
            • Strube, K., et al. 2007. Journal of Biological Chemistry 282, 20292.
            • Satoshi Tasumi and Gerardo R. Vasta. 2007. Journal of Immunology 179, 3086.
            • Yang, F., et al. 2007. Journal of Biological Chemistry 282, 3856.
            • Yang, M., et al. 2007. Cell , 744.
            • Zhao, K.-H., et al. 2007. Procedings of the National Academy of Science 104, 14300.
            • Gakh, O., et al. 2006. Human Molecular Genetics 15, 467.
            • Heraud, J.-M., et al. 2006. Journal of immunology 177, 2552.
            • Janjic, B., et al. 2006. Journal of Immunology 177, 2717.
            • Alex Kudrin, A., et al. 2006. Journal of Biological Chemistry 281, 29641.
            • Park, S.-A., et al. 2006. Procedings of the National Academy of Science 103, 11886.
            • Rachelle M. Smith and Stanly B. Williams. 2006. Proceedings of the National Academy of Sciences (USA) 103, 8564.
            • Sosnovtsev, S.V., et al. 2006. Journal of Virology 80, 7816.
            • Stewart, J.J., et al. 2006. Journal of Biological Chemistry 281, 22665.
            • Alphey, M.S., et al. 2005. Journal of Biological Chemistry 280, 3068.
            • Christophe Anjard and William F. Loomis. 2005. Procedings of the National Academy of Science 102, 7607.
            • Bachler, C., et al. 2005. European Molecular Biology Organization Journal 24, 283.
            • Banki, M.,R., et al. 2005. Protein Science 14, 1387.
            • Bausch, S.L., et al. 2005. Journal of Biological Chemistry 280, 29956.
            • Birgander, P.L., et al. 2005. Journal of Biological Chemistry 280, 14997.
            • Bonner, E.R., et al. 2005. Journal of Biological Chemistry 280, 38803.
            • Leisl A. Brand and Erick Strauss. 2005. Journal of Biological Chemistry 280, 20185.
            • Brouns, S.J.J., et al. 2005. Journal of Biological Chemistry 280, 11422.
            • Bruck, I., et al. 2005. Journal of Biological Chemistry 280, 18152.
            • van Bueren, A.L., et al. 2005. Journal of Biological Chemistry 280, 530.
            • Carmelo, E., et al. 2005. Journal of Biological Chemistry 280, 28683.
            • Casarosa, P., et al. 2005. Journal of Biological Chemistry 280, 3275.
            • Pedro Castanheira, P., et al. 2005. Journal of Biological Chemistry 280, 13047.
            • Chen, M-H., et al. 2005. Journal of Biological Chemistry 280, 10599.
            • Veronika Csizmok, V., et al. 2005. Molecular and Cellular Proteomics 5, 265.
            • Davidson, D.J., et al. 2005. Cancer Research 65, 4663.
            • Eggler, A.L., et al. 2005. Proceedings of the National Academy of Sciences (USA) 102, 10070.
            • Fierobe, H-P., et al. 2005. Journal of Biological Chemistry 280, 16325.
            • Flaig, R., et al. 2005. Journal of Biological Chemistry 280, 19250.
            • Francis, K., et al. 2005. Journal of Biological Chemistry 280, 5195.
            • Fukuda, A., et al. 2005. Plant and Cell Physiology 46, 1779.
            • Gómez-Merino, F.C., et al. 2005. Journal of Biological Chemistry 280, 34888.
            • Georgescu, S.P., et al. 2005. Molecular Endocrinology 19, 2491.
            • Peter Gin and Catherine F. Clarke. 2005. Journal of Biological Chemistry 280, 2676.
            • Guo, R-T., et al. 2005 Journal of Biological Chemistry 280, 20762.
            • Han, J-M., et al. 2005. Journal of Lipid Research 46, 589.
            • Nishiwaki, H., et al. 2007. Applied and Enviornmental Microbiology 73, 3404-.
            • MichelOtte, M.M., et al. 2007. Journal of Bacteriology 189, 7310.
            • Park, M.-O., et al. 2007. Journal of Bacteriology 189, 7281.
            • Qi, M., et al. 2007. Applied and Enviornmental Microbiology 73, 6098.
            • Shchedrina, V.A., et al. 2007. Procedings of the National Academy of Science 104, 13919.
            • Strube, K., et al. 2007. Journal of Biological Chemistry 282, 20292.
            • Satoshi Tasumi and Gerardo R. Vasta. 2007. Journal of Immunology 179, 3086.
            • Yang, F., et al. 2007. Journal of Biological Chemistry 282, 3856.
            • Yang, M., et al. 2007. Cell , 744.
            • Zhao, K.-H., et al. 2007. Procedings of the National Academy of Science 104, 14300.
            • Gakh, O., et al. 2006. Human Molecular Genetics 15, 467.
            • Heraud, J.-M., et al. 2006. Journal of immunology 177, 2552.
            • Janjic, B., et al. 2006. Journal of Immunology 177, 2717.
            • Alex Kudrin, A., et al. 2006. Journal of Biological Chemistry 281, 29641.
            • Park, S.-A., et al. 2006. Procedings of the National Academy of Science 103, 11886.
            • Rachelle M. Smith and Stanly B. Williams. 2006. Proceedings of the National Academy of Sciences (USA) 103, 8564.
            • Sosnovtsev, S.V., et al. 2006. Journal of Virology 80, 7816.
            • Stewart, J.J., et al. 2006. Journal of Biological Chemistry 281, 22665.
            • Alphey, M.S., et al. 2005. Journal of Biological Chemistry 280, 3068.
            • Christophe Anjard and William F. Loomis. 2005. Procedings of the National Academy of Science 102, 7607.
            • Bachler, C., et al. 2005. European Molecular Biology Organization Journal 24, 283.
            • Banki, M.,R., et al. 2005. Protein Science 14, 1387.
            • Bausch, S.L., et al. 2005. Journal of Biological Chemistry 280, 29956.
            • Birgander, P.L., et al. 2005. Journal of Biological Chemistry 280, 14997.
            • Bonner, E.R., et al. 2005. Journal of Biological Chemistry 280, 38803.
            • Leisl A. Brand and Erick Strauss. 2005. Journal of Biological Chemistry 280, 20185.
            • Brouns, S.J.J., et al. 2005. Journal of Biological Chemistry 280, 11422.
            • Bruck, I., et al. 2005. Journal of Biological Chemistry 280, 18152.
            • van Bueren, A.L., et al. 2005. Journal of Biological Chemistry 280, 530.
            • Carmelo, E., et al. 2005. Journal of Biological Chemistry 280, 28683.
            • Casarosa, P., et al. 2005. Journal of Biological Chemistry 280, 3275.
            • Pedro Castanheira, P., et al. 2005. Journal of Biological Chemistry 280, 13047.
            • Chen, M-H., et al. 2005. Journal of Biological Chemistry 280, 10599.
            • Veronika Csizmok, V., et al. 2005. Molecular and Cellular Proteomics 5, 265.
            • Davidson, D.J., et al. 2005. Cancer Research 65, 4663.
            • Eggler, A.L., et al. 2005. Proceedings of the National Academy of Sciences (USA) 102, 10070.
            • Fierobe, H-P., et al. 2005. Journal of Biological Chemistry 280, 16325.
            • Flaig, R., et al. 2005. Journal of Biological Chemistry 280, 19250.
            • Francis, K., et al. 2005. Journal of Biological Chemistry 280, 5195.
            • Fukuda, A., et al. 2005. Plant and Cell Physiology 46, 1779.
            • Gómez-Merino, F.C., et al. 2005. Journal of Biological Chemistry 280, 34888.
            • Georgescu, S.P., et al. 2005. Molecular Endocrinology 19, 2491.
            • Peter Gin and Catherine F. Clarke. 2005. Journal of Biological Chemistry 280, 2676.
            • Guo, R-T., et al. 2005 Journal of Biological Chemistry 280, 20762.
            • Han, J-M., et al. 2005. Journal of Lipid Research 46, 589.
            • Hanaoka, S., et al. 2005. Protein Science 14, 119.
            • Herman, P.L., et al. 2005. Journal of Biological Chemistry 280, 24759.
            • Heurgue-Hamard, V., et al. 2005. Journal of Biological Che
            • User Protocols

              Title
              TB009 Competent Cells

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              Categories

              Life Science Research > Genomic Analysis > Transfection and Protein Expression > Bacterial Expression > Competent Cells for Expression