391334 | BES, Free Acid, ULTROL® Grade - CAS 10191-18-1 - Calbiochem

391334
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      Overview

      Replacement Information

      Key Specifications Table

      Empirical FormulaCAS #
      C₆H₁₅NO₅S 10191-18-1

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      391334-100GM
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          Glass bottle 100 gm
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          391334-25GM
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              Plastic ampoule 25 gm
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              Description
              OverviewBES buffer is suitable for use in systems where a calcium phosphate-DNA complex formation is desirable. Chen and Okayama described an improved transfection system for the stable transformation of cells with plasmid DNA using BES buffer. The gradual formation of the calcium phosphate-DNA complex and its slow precipitation onto cells appears to be the most critical factor contributing to the high efficiency of stable transformations.
              Absorbance (100 mM, 260 nm):≤0.05.
              Catalogue Number391334
              Brand Family Calbiochem®
              SynonymsN,N-bis-(2-Hydroxyethyl)-2-aminoethanesulfonic Acid
              References
              ReferencesDe Graeve, K.G., et al. 1994. J. Appl. Bacteriol. 76, 55.
              Chen, C.A., and Okayama, H. 1988. Biotechniques 6, 632.
              Product Information
              CAS number10191-18-1
              FormWhite solid
              Hill FormulaC₆H₁₅NO₅S
              Chemical formulaC₆H₁₅NO₅S
              PKA7.12 at 20°C
              Applications
              Biological Information
              Purity≥99% by titration (dry basis)
              Physicochemical Information
              Heavy metals≤1 ppm
              Dimensions
              Materials Information
              Toxicological Information
              Safety Information according to GHS
              RTECSKI7363500
              Safety Information
              Product Usage Statements
              Storage and Shipping Information
              Ship Code Ambient Temperature Only
              Toxicity Standard Handling
              Storage +15°C to +30°C
              Do not freeze Ok to freeze
              Special InstructionsFollowing reconstitution, store in the refrigerator (4°C). Stock solutions are stable for up to 2 weeks at 4°C.
              Packaging Information
              Transport Information
              Supplemental Information
              Specifications

              Documentation

              SDS

              Title

              Safety Data Sheet (SDS) 

              Certificates of Analysis

              TitleLot Number
              391334

              References

              Reference overview
              De Graeve, K.G., et al. 1994. J. Appl. Bacteriol. 76, 55.
              Chen, C.A., and Okayama, H. 1988. Biotechniques 6, 632.

              Brochure

              Title
              Detergents and Buffers Technical Bulletin
              Data Sheet

              Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

              Revision14-July-2008 RFH
              SynonymsN,N-bis-(2-Hydroxyethyl)-2-aminoethanesulfonic Acid
              DescriptionBES buffer is suitable for use in systems where a calcium phosphate-DNA complex formation is desirable (Absorbance: <0.05 at 260 nm, 100 mM). Chen and Okayama describe an improved transfection system for the stable transformation of cells with plasmid DNA using BES buffer. The slow formation appears to be the most critical factor contributing to the high efficiency of stable transformations. Has a pKa of 7.12 at 20°C.
              FormWhite solid
              CAS number10191-18-1
              RTECSKI7363500
              Chemical formulaC₆H₁₅NO₅S
              Purity≥99% by titration (dry basis)
              SolubilityH₂O (1 mg/ml)
              Storage +15°C to +30°C
              Do Not Freeze Ok to freeze
              Special InstructionsFollowing reconstitution, store in the refrigerator (4°C). Stock solutions are stable for up to 2 weeks at 4°C.
              Toxicity Standard Handling
              ReferencesDe Graeve, K.G., et al. 1994. J. Appl. Bacteriol. 76, 55.
              Chen, C.A., and Okayama, H. 1988. Biotechniques 6, 632.