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05-678 | Anti-ubiquityl-Histone H2A Antibody, clone E6C5

200 µL  
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      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, R, M, Mk, Am ICC, WB, ChIP M Purified Monoclonal Antibody
      Catalogue Number05-678
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-ubiquityl-Histone H2A Antibody, clone E6C5
      Alternate Names
      • H2AUb
      • Histone H2A (ubiquityl)
      • H2A histone family, member R
      • histone 1
      • H2aa
      • histone H2A
      • histone cluster 1
      • H2aa
      • H2AK119Ub1
      Background InformationHistones are highly conserved proteins that serve as the structural scaffold for the organization of nuclear DNA into chromatin. Histones are modified post-translationally by acetylation, phosphorylation, methylation, and ubiquitination and these modifications regulate DNA transcription, repair, recombination, and replication. Ubiquitylation usually targets the substrate for degradation, although histones H2A and H2B are actually stabilized by a single ubiquitin conjugation. Histone ubiquitination has been correlated with DNA repair and transcription, cellular differentiation, cell cycle regulation, spermatogenesis, protein trafficking, and response to stress. Histone H2A is monoubiquitinated at Lys119 by the PRC-1L complex (Polycomb repressive complex 1-like), which includes Ring1, Ring2, Bmi1 and HPH2. Ubiquitinated H2A normally represents about 15% of H2A, but this value can be as high as 50% in active chromatin. Ubiquitinyl histone H2A has also been associated with transcriptional silencing of large chromatin regions and linked to Polycomb silencing so the function of ubiquitinated H2A remains undefined.
      Product Information
      • Acid extracts of HeLa cells.
      PresentationPurified mouse monoclonal IgM in buffer containing PBS with 0.05% sodium azide before the addition of glycerol to 30%. Liquid at -20ºC.
      ApplicationUse Anti-ubiquityl-Histone H2A Antibody, clone E6C5 (Mouse Monoclonal Antibody) has been published and validated for use in ChIP, ICC, WB to detect ubiquityl-Histone H2A also known as H2AUb, Histone H2A (ubiquityl), histone H2A.
      Key Applications
      • Immunocytochemistry
      • Western Blotting
      • Chromatin Immunoprecipitation (ChIP)
      Application NotesImmunocytochemistry:
      This antibody has been reported by an independent laboratory to detect ubiquityl-Histone H2A in cells fixed with either 3% formaldehyde/0.1% Triton X-100 or methanol (Vassilev, A. 1995).

      Chromatin Immunoprecipitation:
      Reported by an independent laboratory (Wang, H. 2004).
      Biological Information
      ImmunogenAMA (human epithelial amnion) cell residual nuclear pellet portions.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      SpecificityThis antibody recognizes and is specific for monoubiquityl-Histone H2A (Lys119), Mr ~25 kDa.
      Species Reactivity
      • Human
      • Rat
      • Mouse
      • Monkey
      • Amphibian
      Species Reactivity NoteHuman, mouse, rat, monkey, and frog. Does not cross-react with army worm. Other species not tested.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryHistones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H2A family. [provided by RefSeq].
      Gene Symbol
      • HIST1H2AA
      • H2AFR
      • bA317E16.2
      • Ubiquitylation
      Purification MethodAmmonium Sulfate Precipitation
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q96QV6 # Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
      SIZE: 131 amino acids; 14234 Da
      SUBUNIT: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.
      PTM: The chromatin-associated form is phosphorylated on Thr-121 during mitosis (Probable). & Deiminated on Arg-4 in granulocytes upon calcium entry. & Monoubiquitination of Lys-120 by RING1 and RNF2/RING2 complex gives a specific tag for epigenetic transcriptional repression and participates in X chromosome inactivation of female mammals. It is involved in the initiation of both imprinted and random X inactivation. Ubiquitinated H2A is enriched in inactive X chromosome chromatin. Ubiquitination of H2A functions downstream of methylation of 'Lys-27' of histone H3. Monoubiquitination of Lys-120 by RNF2/RING2 can also be induced by ultraviolet and may be involved in DNA repair. & Phosphorylation on Ser-2 is enhanced during mitosis. Phosphorylation on Ser-2 by RPS6KA5/MSK1 directly represses transcription. Acetylation of H3 inhibits Ser-2 phosphorylation by RPS6KA5/MSK1. & Symmetric dimethylation on Arg-4 by the PRDM1/PRMT5 complex may play a crucial role in the germ-cell lineage (By similarity).
      SIMILARITY: SwissProt: Q96QV6 ## Belongs to the histone H2A family.
      Molecular Weight25 kDa
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by western blot on H2A in acid extracts from HeLa and 10T1/2 cells.

      Western Blot Analysis:
      A 1:500-1:2000 dilution of this lot detected ubiquityl-Histone H2A in acid extracts from HeLa and 10T1/2 cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt. For maximum recovery of product, centrifuge the vial prior to removing the cap.
      Packaging Information
      Material Size200 µL
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-ubiquityl-Histone H2A, clone -2551216 2551216
      Anti-ubiquityl-Histone H2A, clone E6C5 - 2430485 2430485
      Anti-ubiquityl-Histone H2A, clone E6C5 - 1970374 1970374
      Anti-ubiquityl-Histone H2A, clone E6C5 - 2019509 2019509
      Anti-ubiquityl-Histone H2A, clone E6C5 - 2041027 2041027
      Anti-ubiquityl-Histone H2A, clone E6C5 - 2073775 2073775
      Anti-ubiquityl-Histone H2A, clone E6C5 - 2239798 2239798
      Anti-ubiquityl-Histone H2A, clone E6C5 - 22424 22424
      Anti-ubiquityl-Histone H2A, clone E6C5 - DAM1514075 DAM1514075
      Anti-ubiquityl-Histone H2A, clone E6C5 - DAM1523000 DAM1523000
      Anti-ubiquityl-Histone H2A, clone E6C5 - DAM1588211 DAM1588211
      Anti-ubiquityl-Histone H2A, clone E6C5 - DAM1632068 DAM1632068
      Anti-ubiquityl-Histone H2A, clone E6C5 - DAM1682129 DAM1682129
      Anti-ubiquityl-Histone H2A, clone E6C5 - DAM1748603 DAM1748603
      Anti-ubiquityl-Histone H2A, clone E6C5 - DAM1780361 DAM1780361
      Anti-ubiquityl-Histone H2A, clone E6C5 - DAM1797929 DAM1797929
      Anti-ubiquityl-Histone H2A, clone E6C5 - DAM1813172 DAM1813172
      Anti-ubiquityl-Histone H2A, clone E6C5 - JBC1500604 JBC1500604
      Anti-ubiquityl-Histone H2A, clone E6C5 - JBC1870058 JBC1870058
      Anti-ubiquityl-Histone H2A, clone E6C5 -2488976 2488976
      Anti-ubiquityl-Histone H2A, clone E6C5 -2594402 2594402
      Anti-ubiquityl-Histone H2A, clone E6C5 -2686896 2686896
      Anti-ubiquityl-Histone H2A, clone E6C5 -2721694 2721694
      Anti-ubiquityl-Histone H2A, clone E6C5 -2780494 2780494

      References | 44 Available | See All References

      Reference overviewApplicationSpeciesPub Med ID
      Epigenetic Regulation of Antibody Responses by the Histone H2A Deubiquitinase MYSM1.
      Jiang, XX; Chou, Y; Jones, L; Wang, T; Sanchez, S; Huang, XF; Zhang, L; Wang, C; Chen, SY
      Scientific reports 5 13755 2015

      Show Abstract
      26348977 26348977
      Impact of flanking chromosomal sequences on localization and silencing by the human non-coding RNA XIST.
      Kelsey, AD; Yang, C; Leung, D; Minks, J; Dixon-McDougall, T; Baldry, SE; Bogutz, AB; Lefebvre, L; Brown, CJ
      Genome biology 16 208 2015

      Show Abstract
      26429547 26429547
      The EBNA3 family of Epstein-Barr virus nuclear proteins associates with the USP46/USP12 deubiquitination complexes to regulate lymphoblastoid cell line growth.
      Ohashi, M; Holthaus, AM; Calderwood, MA; Lai, CY; Krastins, B; Sarracino, D; Johannsen, E
      PLoS pathogens 11 e1004822 2015

      Show Abstract
      25855980 25855980
      The histone H2A deubiquitinase Usp16 regulates embryonic stem cell gene expression and lineage commitment.
      Yang, W; Lee, YH; Jones, AE; Woolnough, JL; Zhou, D; Dai, Q; Wu, Q; Giles, KE; Townes, TM; Wang, H
      Nature communications 5 3818 2014

      Show Abstract
      24784029 24784029
      USP3 counteracts RNF168 via deubiquitinating H2A and γH2AX at lysine 13 and 15.
      Sharma, N; Zhu, Q; Wani, G; He, J; Wang, QE; Wani, AA
      Cell cycle (Georgetown, Tex.) 13 106-14 2014

      Show Abstract
      Western Blotting24196443 24196443
      Impact of human MLL/COMPASS and polycomb complexes on the DNA methylome.
      Putiri, EL; Tiedemann, RL; Liu, C; Choi, JH; Robertson, KD
      Oncotarget 5 6338-52 2014

      Show Abstract
      Western Blotting25071008 25071008
      BMI1 reprogrammes histone acetylation and enhances c-fos pathway via directly binding to Zmym3 in malignant myeloid progression.
      Shen, H; Chen, Z; Ding, X; Qi, X; Cen, J; Wang, Y; Yao, L; Chen, Y
      Journal of cellular and molecular medicine 18 1004-17 2014

      Show Abstract
      24571310 24571310
      Ubiquitin-specific peptidase 42 (USP42) functions to deubiquitylate histones and regulate transcriptional activity.
      Hock, AK; Vigneron, AM; Vousden, KH
      The Journal of biological chemistry 289 34862-70 2014

      Show Abstract
      Western Blotting25336640 25336640
      USP49 deubiquitinates histone H2B and regulates cotranscriptional pre-mRNA splicing.
      Zhang, Z; Jones, A; Joo, HY; Zhou, D; Cao, Y; Chen, S; Erdjument-Bromage, H; Renfrow, M; He, H; Tempst, P; Townes, TM; Giles, KE; Ma, L; Wang, H
      Genes & development 27 1581-95 2013

      Show Abstract
      23824326 23824326
      Usp16 contributes to somatic stem-cell defects in Down's syndrome.
      Adorno, M; Sikandar, S; Mitra, SS; Kuo, A; Nicolis Di Robilant, B; Haro-Acosta, V; Ouadah, Y; Quarta, M; Rodriguez, J; Qian, D; Reddy, VM; Cheshier, S; Garner, CC; Clarke, MF
      Nature 501 380-4 2013

      Show Abstract
      Mouse24025767 24025767


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