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07-018-I | Anti-phospho-p70 S6 Kinase (Thr389) Antibody

100 µg  
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      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H WB, PIA Rb Affinity Purified Polyclonal Antibody
      Catalogue Number07-018-I
      DescriptionAnti-phospho-p70 S6 Kinase (Thr389) Antibody
      Alternate Names
      • Ribosomal protein S6 kinase
      • S6K-beta-1
      • S6K1
      • p70S6K1
      • p70 S6K1
      • p70-S6K1
      • p70 S6KA
      Background InformationThe p70 S6 kinase is a ubiquitous cytoplasmic protein that is activated in response to cytokines, and growth factors including the epidermal growth factor and fibroblast growth factor. It lies downstream of the mTOR/PI3K pathway and is phosphorylated on multiple residues: Thr389, Thr229, Thr421, Ser371, Ser404, Ser411, Ser418, and Ser424. Thr389, Ser371, and Ser404 are located in the linker domain; whereas Thr421, Ser411, Ser418, Ser424 are located in the autoinhibitory domain, and Thr229 is found in the catalytic domain. These sites may be phosphorylated in a hierarchical sequence leading to the activation of the p70 S6 kinase. The p70 S6 kinase phosphorylates the S6 protein on the 40S ribosomal protein (rpS6), and plays a role in multiple processes including regulation of mRNA molecules with 5’-terminal oligopyrimidine tracts, protein synthesis, cell growth, proliferation, and cell survival. The activity of this kinase may be important in diabetes, cancer, and aging.
      Product Information
      FormatAffinity Purified
      • MCF7 cell lysate
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      ApplicationDetect phospho-p70 S6 Kinase (Thr389) using this Anti-phospho-p70 S6 Kinase (Thr389) Antibody validated for use in Western Blotting, Peptide Inhibition Assay.
      Key Applications
      • Western Blotting
      • Peptide Inhibition Assay
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to the AGC-kinase C-terminal of human Ribosomal protein S6 kinase beta-1
      phosphorylated at Thr389.
      EpitopeAGC-kinase C-terminal
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      SpecificityThis antibody recognizes the AGC-kinase C-terminal of Ribosomal protein S6 kinase beta-1 phosphorylated at Thr389.
      Species Reactivity
      • Human
      Species Reactivity NoteDemonstrated to react with Human. Predicted to react with Mouse, Rat, Chicken, Zebra Fish, and Bovine based on 100% sequence homology. Other homologies: ribosomal protein S6 kinase beta-2 (91% sequence homology).
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • RPS6KB1
      • STK14A
      Purification MethodAffinity Purfied
      UniProt Number
      Molecular Weight~70 kDa observed
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot (Peptide Inhibition) Analysis in MCF7 cell lysate and MCF7 cell lysate with non-phospho or phospho peptide.

      Western Blot (Peptide Inhibition) Analysis: 1.0 µg/mL of this antibody detected p70 S6 Kinase phosphorylated at Thr389 in 10 µg of MCF7 cell lysate and MCF7 cell lysate incubated with a non-phospho peptide.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information

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      Life Science Research > Antibodies and Assays > Primary Antibodies