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07-562 | Anti-phospho-eNOS/NOS III (Ser635) Antibody

200 µL  
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      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      B WB Rb Affinity Purified Polyclonal Antibody
      Catalogue Number07-562
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-phospho-eNOS/NOS III (Ser635) Antibody
      OverviewNote: ADMA (Catalog #19-169) and NMMA (Catalog #19-170) are inhibitors of NOS
      Product Information
      FormatAffinity Purified
      Presentation0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide before the addition of glycerol to 30%
      ApplicationAnti-phospho-eNOS/NOS III (Ser635) Antibody is an antibody against phospho-eNOS/NOS III (Ser635) for use in WB.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenPeptide corresponding to amino acids surrounding phosphorylated Ser635 of bovine eNOS/NOS III. This site corresponds to human Ser633.
      Specificityphosphorylated eNOS/NOS III (Ser635)
      Species Reactivity
      • Bovine
      Species Reactivity NotePredicted to cross-react with human, mouse and canine based on sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • NOS3
      • cNOS
      • ECNOS
      • eNOS
      • EC-NOS
      • NOSIII
      • Phosphorylation
      Purification MethodImmunoAffinity Purified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P29474 # Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. No mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.
      COFACTOR: Heme group. & Binds 1 FAD. & Binds 1 FMN. & Metrahydrobiopterin (BH4). May stabilize the dimeric form of the enzyme.
      SIZE: 1203 amino acids; 133289 Da
      SUBUNIT: Homodimer. Interacts with NOSIP and NOSTRIN.
      SUBCELLULAR LOCATION: Cell membrane, caveola. Cytoplasm, cytoskeleton. Golgi apparatus. Note=Specifically associates with actin cytoskeleton in the G2 phase of the cell cycle; which is favored by interaction with NOSIP and results in a reduced enzymatic activity.
      TISSUE SPECIFICITY: Platelets, placenta, liver and kidney.
      SIMILARITY: SwissProt: P29474 ## Belongs to the NOS family. & Contains 1 flavodoxin-like domain.
      Molecular Weight132kDa
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by immunoblot in RIPA lysates from fetal bovine heart endothelial (FBHE) cells
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions1 year at -20°C
      Packaging Information
      Material Size200 µL
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-phospho- eNOS/NOS III (Ser635) (rabbit immunoaffinity purified IgG) - DAM1437154 DAM1437154
      Anti-phospho- eNOS/NOS III (Ser635) -2776757 2776757
      Anti-phospho-eNOS/NOS III (Ser635) - 26837 26837


      Reference overviewPub Med ID
      Tongxinluo reduces myocardial no-reflow and ischemia-reperfusion injury by stimulating the phosphorylation of eNOS via the PKA pathway.
      Li, XD; Yang, YJ; Geng, YJ; Jin, C; Hu, FH; Zhao, JL; Zhang, HT; Cheng, YT; Qian, HY; Wang, LL; Zhang, BJ; Wu, YL
      American journal of physiology. Heart and circulatory physiology 299 H1255-61 2010

      Show Abstract
      20693395 20693395
      Src kinase activates endothelial nitric-oxide synthase by phosphorylating Tyr-83.
      Fulton, D; Church, JE; Ruan, L; Li, C; Sood, SG; Kemp, BE; Jennings, IG; Venema, RC
      The Journal of biological chemistry 280 35943-52 2005

      Show Abstract
      16123043 16123043
      Compensatory phosphorylation and protein-protein interactions revealed by loss of function and gain of function mutants of multiple serine phosphorylation sites in endothelial nitric-oxide synthase.
      Bauer, Philip M, et al.
      J. Biol. Chem., 278: 14841-9 (2003) 2003

      12591925 12591925
      Shear stress stimulates phosphorylation of eNOS at Ser(635) by a protein kinase A-dependent mechanism.
      Boo, Yong Chool, et al.
      Am. J. Physiol. Heart Circ. Physiol., 283: H1819-28 (2002) 2002

      12384459 12384459
      Identification of regulatory sites of phosphorylation of the bovine endothelial nitric-oxide synthase at serine 617 and serine 635.
      Michell, Belinda J, et al.
      J. Biol. Chem., 277: 42344-51 (2002) 2002

      Show Abstract
      12171920 12171920


      Pathways and Biomarkers of Oxidative Stress (EMD)

      Technical Info

      White Paper- Modern Methods in Oxidative Stress Research (EMD)