A part of MilliporeSigma

07-1426 | Anti-phospho-VEGFR2/Flk-1/KDR (Tyr996) Antibody

100 µL  
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      Replacement Information
      Replacement Information07-1426 is a recommended replacement for AB3847

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M WB, IP Rb Purified Polyclonal Antibody
      Catalogue Number07-1426
      DescriptionAnti-phospho-VEGFR2/Flk-1/KDR (Tyr996) Antibody
      Alternate Names
      • Protein-tyrosine kinase receptor Flk-1
      • Vascular endothelial growth factor receptor
      Background InformationThe Flt gene family of receptor tyrosine kinases includes Flt-1, KDR/Flk-1, Flt-3, and Flt-4. They encode receptors for VEGF, characterized by seven Ig-loops within their extracellular domain and a split kinase domain within the cytoplasmic domain. VEGF is an important angiogenic factor of many solid tumors and is also involved in the differentiation of endothelial cells. Flt-1 (Fms-like tyrosine kinase, VEGF-R1) is expressed on endothelial cells and on the common precursors of endothelial and hematopoietic stem cells. KDR (Flk-1, VEGF-R2) is the principal receptor involved in VEGF signaling. KDR is expressed on endothelial cells and stimulates angiogenesis, but is also expressed on bone marrow cells, and is thought to play a role in driving the proliferation of most or all leukemic cells. Flt3 (also known as Flk-2) is a class III receptor tyrosine kinase that is closely related to c-Kit and c-Fms. It is expressed in primitive hematopoietic cells and its ligand exerts proliferative effects on these cells in co-operation with other cytokines.
      Product Information
      • Huvec cell lysate
      PresentationPurified rabbit polyclonal IgG in buffer containing 10 mM sodium HEPES, pH 7.5, 150 mM NaCl, 100 µg/mL BSA, with 50% glycerol.
      ApplicationAnti-phospho-VEGFR2/Flk-1/KDR (Tyr996) Antibody is an antibody against phospho-VEGFR2/Flk-1/KDR (Tyr996) for use in WB & IP.
      Key Applications
      • Western Blotting
      • Immunoprecipitation
      Application NotesImmunoprecipitation: 1:50 dilution from a previous lot of this antibody was used in immunoprecipitation.

      Optimal working dilutions must be determined by the end user.
      Biological Information
      ImmunogenKLH-conjugated, synthetic phospho-peptide corresponding to residues surrounding Tyr996 of human VEGF receptor-2.
      SpecificityRecognizes VEGFR-2 only when phosphorylated at tyrosine 996. This antibody cross-reacts with tyrosine-phosphorylated PDGF receptor but does not cross-react with other tyrosine-phosphorylated proteins.
      Species Reactivity
      • Human
      • Mouse
      Species Reactivity NoteHuman. Predicted to react with mouse due to 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • KDR
      • VEGFR-2
      • VEGFR2
      • CD309
      • FLK1
      • VEGFR
      • EC
      • Phosphorylation
      Purification MethodProtein A and Affinity Purified
      UniProt Number
      UniProt SummaryFUNCTION: Receptor for VEGF or VEGFC. Has a tyrosine-protein kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.
      CATALYTIC ACTIVITY: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.
      SUBUNIT: Interacts with SHB upon VEGF activation. Interacts with HIV-1 Tat.
      SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
      SIMILARITY: Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
      SIMILARITY: Contains 7 Ig-like C2-type (immunoglobulin-like) domains.
      SIMILARITY: Contains 1 protein kinase domain.
      Molecular Weight~140 kDa
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by Western Blot on pervanadate treated Huvec lysates.

      Western Blot Analysis:
      1:500-1:2,000 dilution of this lot detected phosphorylated VEGFR2 on 10 μg of pervanadate treated Huvec lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20ºC from date of receipt, do not aliquot.
      Handling Recommendations: Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerolcontaining solutions to become frozen during storage. Note: Variability in freezer temperatures below -20°C may cause glycerolcontaining solutions to become frozen during storage.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information

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      Life Science Research > Antibodies and Assays > Primary Antibodies