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ABE1971 | Anti-phospho TFEB (Ser142) Antibody

ABE1971
100 µL  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H WB Rb Affinity Purified Polyclonal Antibody
      Description
      Catalogue NumberABE1971
      DescriptionAnti-phospho TFEB (Ser142) Antibody
      Alternate Names
      • Transcription factor EB, Ser142 phosphorylated
      • bHLHe35, Ser142 phosphorylated
      • Class E basic helix-loop-helix protein 35, Ser142 phosphorylated
      Background InformationTranscription factor EB (UniProt P19484; also known as bHLHe35, Class E basic helix-loop-helix protein 35) is encoded by the TFEB (also known as ALPHATFEB, BHLHE35, TCFEB) gene (Gene ID 7942) in human. TFEB is a basic Helix-Loop-Helix (bHLH) leucine zipper transcription factor that plays a major role in the regulation of lysosomal biogenesis. TFEB is also a main player in the transcriptional response to starvation and controls autophagy by positively regulating autophagosome formation and autophagosome-lysosome fusion. TFEB is phosphorylated by mTORC1 and ERK at Ser142, which prevents its nuclear translocation. Pharmacological inhibition of mTORC1, as well as starvation and lysosomal disruption, activates TFEB nuclear translocation. Similarly, the serine-threonine kinase RIP1/RIPK1 is shown to suppress basal autophagic flux by activating ERK, which in turn phosphorylates TFEB at Ser142 and thereby prevents TFEB-mediated transcription of gene products involved in autophagy.
      References
      Product Information
      FormatAffinity Purified
      PresentationPurified rabbit polyclonal antibody in PBS with 0.05% sodium azide and 50% glycerol.
      Applications
      ApplicationAnti-phospho TFEB (Ser142) Antibody is an antibody against phospho TFEB for use in Western Blotting.
      Key Applications
      • Western Blotting
      Application NotesWestern Blotting Analysis: A representative lot detected Ser142 phosphorylated TFEB in control HeLa cells, but not in RIP1-deficient HeLa cells following RIP1 shRNAs treatment (Yonekawa, T., et al. (2015). Nat. Cell Biol. EMBO Rep. 16(6):700-708).
      Western Blotting Analysis: A representative lot detected Ser142 phosphorylation induction of exogenously expressed wild-type, but not S142A mutant, TFEB (with 3xFLAG tag) upon amino acids addition to nutrient starved HEK293T transfectants (Settembre, C., et al. (2012). EMBO J. 31(5):1095-1108).
      Western Blotting Analysis: A representative lot detected nutrient-induced Ser142 phosphorylation of exogenously expressed TFEB (with 3xFLAG tag) in starved HeLa transfectants. Pretreatment of mTOR inhibitor Torin1 (Cat. No. 475991) completely prevented nutrient-induced TFEB Ser142 phosphorylation, a weaker mTOR inhibitor Rapamycin (Cat. No. 553210) partially supperessed the Ser142 phosphorylation induciton (Settembre, C., et al. (2012). EMBO J. 31(5):1095-1108).
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to a human TFEB sequence containing the phosphorylated Ser142.
      EpitopepSer142.
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis polyclonal antibody detected a target band only in lysates from cells expressing wild-type TFEB, but not cells expressing TFEB with S142A mutation (Yonekawa, T., et al. (2015). Nat. Cell Biol. EMBO Rep. 16(6):700-708). The target phosphorylation site is conserved in murine (Ser141; UniProt Q9R210) and rat (Ser142; UniProt F7F5J1) TFEB protein, but not present in human TFEB spliced isoform 2 (UniProt P19484-2).
      Species Reactivity
      • Human
      Species Reactivity NoteHuman. Predicted to react with Mouse, Rat, Bovine, Rabbit, and Non-human primates based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • TFEB
      • ALPHATFEB
      • BHLHE35
      • TCFEB
      Modifications
      • Phosphorylation
      Purification MethodAffinity Purfied
      UniProt Number
      Molecular Weight~60 kDa observed (with 3xFLAG tag). 52.87 kDa calculated. Uncharacterized band(s) may appear in some lysates.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in lysates from TFEB-transfected HEK293 cells.

      Western Blotting Analysis: A 1:12500 dilution of this antibody detected Ser142 phosphorylation of FLAG-tagged human TFEB in 10 µg lysate from transfecgted HEK293 cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies