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MABE16 | Anti-phospho Sir2/SIRT1 (Ser47) Antibody, clone EPR2849Y, rabbit monoclonal

100 µL  
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      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H WB, IHC, ICC Rb Unpurified Monoclonal Antibody
      Catalogue NumberMABE16
      DescriptionAnti-phospho Sir2/SIRT1 (Ser47) Antibody, clone EPR2849Y, rabbit monoclonal
      Alternate Names
      • SIR2-like protein 1
      • sirtuin (silent mating type information regulation 2 homolog) 1 (S. cerevisiae)
      • sirtuin (silent mating type information regulation 2, S. cerevisiae, homolog) 1
      Background InformationThe S. cerevisiae silent information regulator 2 (Sir2) protein is an NAD+-dependent histone deacetylase (HDACs) that plays a critical role in transcriptional silencing, genome stability, and longevity (1-2). Part of the Sirtuin family (Sir2, Sir3, Sir3), yeast Sir2 is the founding member of Class III HDACs. A human homologue of Sir2, SIRT1, regulates the activity of the p53 tumor suppressor and inhibits apoptosis (3-4).
      Product Information
      • Lambda phosphatase (LP)-treated and untreated 293 cell lysates.
      PresentationRabbit monoclonal in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl containing 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
      ApplicationUse Anti-phospho Sir2/SIRT1 (Ser47) Antibody, clone EPR2849Y (Rabbit Monoclonal Antibody) validated in WB, IHC, ICC to detect phospho Sir2/SIRT1 (Ser47) also known as SIR2-like protein 1 or sirtuin 1.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      • Immunocytochemistry
      Application NotesImmunohistochemistry Analysis: A 1:250 – 1:500 dilution from a previous lot detected Sir2/SIRT1 in IH.
      Immunocytochemistry Analysis: A 1:100 – 1:250 dilution from a previous lot detected Sir2/SIRT1 in IC.
      Biological Information
      ImmunogenPhospho-specific peptide corresponding to human Sir2/SIRT1 at Ser47.
      SpecificityThis antibody detects Sir2/SIRT1 phosphorylated on Ser47.
      Species Reactivity
      • Human
      Species Reactivity NoteProven to react with human.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • sirtuin 1
      • SIR2L1
      • hSIR2
      • hSIRT1
      • Phosphorylation
      Purification MethodUnpurified
      UniProt Number
      UniProt SummaryFUNCTION: NAD-dependent protein deacetylase, which regulates processes such as apoptosis and muscle differentiation by deacetylating key proteins. Deacetylates 'Lys-382' of p53/TP53 and impairs its ability to induce proapoptotic program and modulate cell senescence. Deacetylates TAF1B and thereby represses rDNA transcription by the RNA polymerase I. Involved in HES1- and HEY2-mediated transcriptional repression. Inhibits skeletal muscle differentiation by deacetylating PCAF and MYOD1. May serve as a sensor of the cytosolic ratio of NAD+/NADH, which is essential in skeletal muscle cell differentiation. Deacetylates 'Lys-16' of histone H4 (in vitro). Component of the eNoSC (energy-dependent nucleolar silencing) complex, a complex that mediates silencing of rDNA in response to intracellular energy status and acts by recruiting histone-modifying enzymes. The eNoSC complex is able to sense the energy status of cell: upon glucose starvation, elevation of NAD+/NADP+ ratio activates SIRT1, leading to histone H3 deacetylation followed by dimethylation of H3 at 'Lys-9' (H3K9me2) by SUV39H1 and the formation of silent chromatin in the rDNA locus. In case of HIV-1 infection, interacts with and deacetylates the viral Tat protein.
      CATALYTIC ACTIVITY: NAD+ + an acetylprotein = nicotinamide + O-acetyl-ADP-ribose + a protein.
      COFACTOR: Binds 1 zinc ion per subunit By similarity.
      ENZYME REGULATION: Inhibited by nicotinamide. Activated by resveratrol (3,5,4'-trihydroxy-trans-stilbene), butein (3,4,2',4'-tetrahydroxychalcone), piceatannol (3,5,3',4'-tetrahydroxy-trans-stilbene), Isoliquiritigenin (4,2',4'-trihydroxychalcone), fisetin (3,7,3',4'-tetrahydroxyflavone) and quercetin (3,5,7,3',4'-pentahydroxyflavone). RPS19BP1/AROS acts as a positive regulator of deacetylation activity. Negatively regulated by KIAA1967/DBC1.
      SUBUNIT STRUCTURE: Interacts with TAF1B. Found in a complex with PCAF and MYOD1 By similarity. Component of the eNoSC complex, composed of SIRT1, SUV39H1 and RRP8. Interacts with MLLT7/FOXO4, HES1, HEY2, p53/TP53 and PML. Interacts with RPS19BP1/AROS. Interacts with KIAA1967/DBC1 (via N-terminus).
      SUBCELLULAR LOCATION: Nucleus › PML body. Note: Recruited to the nuclear bodies via its interaction with PML.
      TISSUE SPECIFICITY: Widely expressed.
      MISCELLANEOUS: Red wine, which contains resveratrol, may participate in activation of sirtuin proteins, and may therefore participate in an extended lifespan as it has been observed in yeast.
      SEQUENCE SIMILARITIES: Belongs to the sirtuin family.
      Contains 1 deacetylase sirtuin-type domain.
      SEQUENCE CAUTIONS: The sequence AAH12499.1 differs from that shown. Reason: Erroneous initiation.
      Molecular Weight~110 kDa observed
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in LP treated and untreated 293 cell lysate.
      Western Blot Analysis: A 1:1,000 – 1:2,000 dilution of this antibody detected Sir2/SIRT1 in LP treated and untreated 293 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information

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      Life Science Research > Antibodies and Assays > Primary Antibodies