07-081 Anti-phospho (Ser10)-acetyl (Lys14)-Histone H3 Antibody

07-081
100 µL  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, H, Eu ICC, WB, ChIP-seq, ChIP Rb Affinity Purified Polyclonal Antibody
      Description
      Catalogue Number07-081
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-phospho (Ser10)-acetyl (Lys14)-Histone H3 Antibody
      Alternate Names
      • H3K14me3S10P
      • Histone H3 (acetyl K14, phospho S10)
      • H3 histone family, member T
      • histone 3
      • H3
      • histone cluster 3
      • H3
      Background InformationHistone H3.1 (UniProt: Q16695; also known as H3K14AcS10P, Histone H3 (acetyl K14, phospho S10), H3 histone family member T, Histone 3, H3, Histone cluster 3, H3) is encoded by the HIST3H3 (also known as HIST1H3A, H3FA, HIST1H3B, H3FL, HIST1H3C, H3FC, HIST1H3D, H3FB, HIST1H3E, H3FD, HIST1H3F, H3FI, HIST1H3G, H3FH, HIST1H3H, H3FK, HIST1H3I, H3FF, HIST1H3J, H3FJ, HIST3H3, H3FT, H3T, MGC126886) gene (Gene ID: 8350, 8351, 8352, 8353, 8354, 8355, 8356, 8357, 8358, 8968) in human. Histone H3 has two main variants, H3.1 and H3.3, which show different genomic localization patterns in animals. The H3.1 and H3.3 complexes also possess distinct histone chaperones, CAF-1 and HIRA, which play important role in mediating DNA-synthesis-dependent and -independent nucleosome assembly. It has been reported that Histone H3.1 serves as the canonical histone, which is incorporated during DNA replication, whereas H3.3 acts as the replacement histone that can be incorporated outside of S-phase during chromatin-disrupting processes like transcription. Histone H 3.1 is a core component of nucleosome that is present only in mammals and is usually enriched in acetylation of Lysine 15 and demethylation of lysine 10 (HeK9Me2). It is expressed during S phase, then expression decreases significantly as cell division slows down during the process of differentiation. Histone H 3.1 expression is shown to be replication dependent. It s presence at the site of UV-induced DNA damage has also been reported. It has also been shown that H3.1/H4 tetramers do not split and remain intact during replication dependent deposition of H3.1 variant. Phosphorylation at serine 10 by aurora kinase B is reported to mediate the dissociation of HP1 proteins (CBX1, CBX3 and CBX5) from heterochromatin and this phosphorylation is considered to be an essential regulatory mechanism for neoplastic cell transformation. Acetylation of lysine 14 of histone H3 (H3K14ac) is shown to be critical for DNA damage checkpoint activation by directly regulating the compaction of chromatin and by recruiting chromatin remodeling protein complex RSC (Remodels the Structure of Chromatin). Nucleosomes with H3K14ac display higher affinity for purified RSC. (Ref.: Stroud, H., et al (2012). Proc. Natl. Acad. Sci. USA 109(14); 5370-75; Dunn, MR., and Smerdon MJ (2014). J. Biol. Chem. 289(12);8353-63)..
      References
      Product Information
      FormatAffinity Purified
      Control
      • Acid-extracted proteins from serum-starved 10T1/2 cells.
      PresentationPurified rabbit polyclonal IgG in buffer containing 0.2 M Tris-glycine, pH 7.4, 0.15 M NaCl, 0.05% sodium azide, before the addition of glycerol to 30%.
      Applications
      ApplicationAnti-phospho (Ser10)-acetyl (Lys14)-Histone H3, Cat. No. 07-081, is a highly specific rabbit polyclonal antibody that targets Histone H3 phosphorylated on Serine 10 and acetylated on Lysine 14 and has been tested for use in Immunocytochemistry, Peptide Inhibition Assay, ChIP-seq and Chromatin Immuno
      Key Applications
      • Immunocytochemistry
      • Western Blotting
      • ChIP-seq
      • Chromatin Immunoprecipitation (ChIP)
      Application NotesWestern Blot Analysis:
      1:2000-1:10,000 dilutions of a previous lot detected phosphorylated and acetylated histone H3 in acid extracted proteins from serum starved mouse 10T1/2 cells treated with 50ng/ml EGF for 12.5 minutes. Western blotting of this lot to acid extracted proteins described above was preferentially competed by peptides in the following order: phospho-acetyl-H3 (7-20) > straight chain H3 (7-20) > phospho-H3 (7-20) or acetyl-H3 (7-20).
      Peptide Inhibition Analysis: A 1:500 dilution from a representative lot was used with HeLa AE for peptide block analysis.
      Chromatin Immunoprecipitation:
      A previous lot of this antibody was reported by an independent laboratory to immunoprecipitate chromatin. (Cheung, P., 2000.)

      Immunocytochemistry:
      A previous lot of this antibody detected phosphorylated and acetylated histone H3 in EGF-stimulated cells; as reported by an independent laboratory. (Cheung, P., 2000.)
      Biological Information
      ImmunogenKLH-conjugated, synthetic peptide corresponding to amino acids 7-20 of human histone H3 (ARK[pS]TGGAcKAPRKQL-C).
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityRecognizes Histone H3 phosphorylated at serine 10 and acetylated at lysine 14, Mr 17 kDa. An unidentified protein is also seen in some cells lines, Mr 40 kDa.
      IsotypeIgG
      Species Reactivity
      • Mouse
      • Human
      • Eukaryote
      Species Reactivity NoteHuman and mouse; broad species cross-reactivity expected.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryHistones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
      Gene Symbol
      • HIST1H3A
      • H3FA
      • HIST1H3B
      • H3FL
      • HIST1H3C
      • H3FC
      • HIST1H3D
      • H3FB
      • HIST1H3E
      • H3FD
      • HIST1H3F
      • H3FI
      • HIST1H3G
      • H3FH
      • HIST1H3H
      • H3FK
      • HIST1H3I
      • H3FF
      • HIST1H3J
      • H3FJ
      • HIST3H3
      • H3FT
      • H3T
      • MGC126886
      Modifications
      • Phosphorylation
      Purification MethodImmunoaffinity and reverse-affinity chromatography.
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q16695 # Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
      SIZE: 136 amino acids; 15508 Da
      SUBUNIT: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.
      SUBCELLULAR LOCATION: Nucleus.
      TISSUE SPECIFICITY: Expressed in testicular cells.
      DEVELOPMENTAL STAGE: Expressed during S phase, then expression strongly decreases as cell division slows down during the process of differentiation.
      PTM: Acetylation is generally linked to gene activation. Acetylation on Lys-10 impairs methylation at Arg-9. Acetylation on Lys-19 and Lys-24 favors methylation at Arg-18 (By similarity). & Citrullination at Arg-9 and/or Arg-18 by PADI4 impairs methylation and represses transcription (By similarity). & Asymmetric dimethylation at Arg-18 by CARM1 is linked to gene activation. Symmetric dimethylation at Arg-9 by PRMT5 is linked to gene repression (By similarity). & Methylation at Lys-5, Lys-37 and Lys-80 are linked to gene activation. Methylation at Lys-5 facilitates subsequent acetylation of H3 and H4. Methylation at Lys-80 is associated with DNA double-strand break (DSB) responses and is a specific target for TP53BP1. Methylation at Lys-10 and Lys-28 are linked to gene repression. Methylation at Lys-10 is a specific target for HP1 proteins (CBX1, CBX3 and CBX5) and prevents subsequent phosphorylation at Ser-11 and acetylation of H3 and H4. Methylation at Lys-5 and Lys-80 require preliminary monoubiquitination of H2B at 'Lys-120'. Methylation at Lys-10 and Lys-28 are enriched in inactive X chromosome chromatin (By similarity). & Phosphorylated at Thr-4 by GSG2/haspin during prophase and dephosphorylated during anaphase. At centromeres, specifically phosphorylated at Thr-12 from prophase to early anaphase. Phosphorylated at Ser-11 during the whole mitosis. Phosphorylation at Ser-11, which is linked to gene activation, prevents methylation at Lys-10 but facilitates acetylation of H3 and H4. Phosphorylated at Ser-29 by MLTK isoform 1, RPS6KA5 or AURKB during mitosis or upon ultraviolet B irradiation (By similarity). & Phosphorylation at 'Ser-11' is crucial for chromosome condensation and cell-cycle progression during mitosis and meiosis. In addition phosphorylation at 'Ser-11' is important during interphase because it enables the transcription of genes following external stimulation, like stress or growth factors. Phosphorylation at 'Ser-11' is also an essential regulatory mechanism for neoplastic cell transformation. Phosphorylation at 'Ser-11' by AURKB/Aurora-B mediates the dissociation of HP1 proteins (CBX1, CBX3 and CBX5) from heterochromatin. & Ubiquitinated (By similarity).
      SIMILARITY: SwissProt: Q16695 ## Belongs to the histone H3 family.
      Molecular Weight17 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by Western Blot on HeLa acid extract.

      Western Blot Analysis:
      1:500 dilution of this lot detected phospho acetyl Histone H3 on 10 μg of HeLa acid extract.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-phospho (Ser10)-acetyl (Lys14)-Histone H3 Antibody SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-phospho (Ser10)-acetyl (Lys14)-Histone H3 Antibody Certificates of Analysis

      TitleLot Number
      Anti-phospho (Ser10)-acetyl (Lys14)- Histone H3 - 19669491966949
      Anti-phospho (Ser10)-acetyl (Lys14)- Histone H3 - 20205442020544
      Anti-phospho (Ser10)-acetyl (Lys14)- Histone H3 - 22009292200929
      Anti-phospho (Ser10)-acetyl (Lys14)- Histone H3 - DAM1644562DAM1644562
      Anti-phospho (Ser10)-acetyl (Lys14)- Histone H3 - DAM1821121DAM1821121
      Anti-phospho (Ser10)-acetyl (Lys14)- Histone H3 - JBC1900550JBC1900550
      Anti-phospho (Ser10)-acetyl (Lys14)-Histone H3 - 23910662391066
      Anti-phospho (Ser10)-acetyl (Lys14)-Histone H3 - 24335422433542
      Anti-phospho (Ser10)-acetyl (Lys14)-Histone H3 - 2010620106
      Anti-phospho (Ser10)-acetyl (Lys14)-Histone H3 - 2086120861

      References

      Reference overviewApplicationPub Med ID
      Human leukocyte antigen-G is frequently expressed in glioblastoma and may be induced in vitro by combined 5-aza-2'-deoxycytidine and interferon-γ treatments: results from a multicentric study.
      Wastowski, IJ; Simões, RT; Yaghi, L; Donadi, EA; Pancoto, JT; Poras, I; Lechapt-Zalcman, E; Bernaudin, M; Valable, S; Carlotti, CG; Flajollet, S; Jensen, SS; Ferrone, S; Carosella, ED; Kristensen, BW; Moreau, P
      The American journal of pathology  182  540-52  2013

      Show Abstract
      23219427 23219427
      Repeated social defeat selectively increases δFosB expression and histone H3 acetylation in the infralimbic medial prefrontal cortex.
      Hinwood, M; Tynan, RJ; Day, TA; Walker, FR
      Cerebral cortex (New York, N.Y. : 1991)  21  262-71  2011

      Show Abstract
      20513656 20513656
      Lovastatin-induced cholesterol depletion affects both apical sorting and endocytosis of aquaporin-2 in renal cells.
      Procino G, Barbieri C, Carmosino M, Rizzo F, Valenti G, Svelto M
      American journal of physiology. Renal physiology  298  F266-78. Epub 2009 Nov 18.  2010

      Show Abstract
      19923410 19923410
      Epigenetic regulation of a murine retrotransposon by a dual histone modification mark.
      Brunmeir, R; Lagger, S; Simboeck, E; Sawicka, A; Egger, G; Hagelkruys, A; Zhang, Y; Matthias, P; Miller, WJ; Seiser, C
      PLoS genetics  6  e1000927  2010

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      Western Blotting20442873 20442873
      14-3-3 mediates histone cross-talk during transcription elongation in Drosophila.
      Karam, CS; Kellner, WA; Takenaka, N; Clemmons, AW; Corces, VG
      PLoS genetics  6  e1000975  2010

      Show Abstract Full Text Article
      Western Blotting20532201 20532201
      Multiple chromatin-bound protein kinases assemble factors that regulate insulin gene transcription.
      Lawrence, MC; Shao, C; McGlynn, K; Naziruddin, B; Levy, MF; Cobb, MH
      Proceedings of the National Academy of Sciences of the United States of America  106  22181-6  2009

      Show Abstract
      20018749 20018749
      Drug-induced activation of dopamine D(1) receptor signaling and inhibition of class I/II histone deacetylase induce chromatin remodeling in reward circuitry and modulate cocaine-related behaviors.
      Schroeder, FA; Penta, KL; Matevossian, A; Jones, SR; Konradi, C; Tapper, AR; Akbarian, S
      Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology  33  2981-92  2008

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      18288092 18288092
      Inhibition of mixed-lineage kinase (MLK) activity during G2-phase disrupts microtubule formation and mitotic progression in HeLa cells.
      Hyukjin Cha, Surabhi Dangi, Carolyn E Machamer, Paul Shapiro
      Cellular signalling  18  93-104  2006

      Show Abstract
      15923109 15923109
      Distinct roles of the steroid receptor coactivator 1 and of MED1 in retinoid-induced transcription and cellular differentiation.
      Flajollet, S; Lefebvre, B; Rachez, C; Lefebvre, P
      The Journal of biological chemistry  281  20338-48  2006

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      16723356 16723356
      Targeted expression of cyclin D2 results in cardiomyocyte DNA synthesis and infarct regression in transgenic mice.
      Pasumarthi, KB; Nakajima, H; Nakajima, HO; Soonpaa, MH; Field, LJ
      Circulation research  96  110-8  2005

      Show Abstract
      Immunohistochemistry15576649 15576649

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