05-368 Anti-phospho-Ser/Thr-Pro MPM-2 Antibody

05-368
200 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      Vrt ELISA, IHC, IP, WB M Purified Monoclonal Antibody
      Description
      Catalogue Number05-368
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-phospho-Ser/Thr-Pro MPM-2 Antibody
      OverviewThis antibody recognizes a variety of proteins that are phosphorylated during mitosis.
      Background InformationUpon entry into M (Mitosis) phase, many proteins are phosphorylated either directly or indirectly by M-phase-promoting factor (MPF). The MPM2 monoclonal antibody binds to a phospho amino acid-containing epitope (peptides containing LTPLK and FTPLQ domains) present on more than 50 proteins of M-phase eukaryotic cells.
      References
      Product Information
      FormatPurified
      Control
      • Colcemid treated HeLa cell lysate or A431 mitotic cells fixed with 3.7% paraformaldehyde
      PresentationProtein G Purified immunoglobulin in Protein G Purified immunoglobulin in 30% glycerol, 0.07M Tris-glycine, pH 7.4, 0.105 M NaCl, 0.035% sodium azide as a preservative.
      Applications
      ApplicationAnti-phospho-Ser/Thr-Pro MPM-2 Antibody is a Mouse Monoclonal Antibody for detection of phospho-Ser/Thr-Pro also known as Mitotic protein #2 & has been tested in ELISA, IHC, IP & WB.
      Key Applications
      • ELISA
      • Immunohistochemistry
      • Immunoprecipitation
      • Western Blotting
      Biological Information
      ImmunogenMitotic human HeLa cell cytosolic lysate
      CloneMPM-2
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityRecognizes phosphorylated serines or threonines when followed by a proline.
      IsotypeIgG1
      Species Reactivity
      • Vertebrates
      Antibody TypeMonoclonal Antibody
      Modifications
      • Phosphorylation
      Purification MethodProtein G Purified
      Molecular Weightvaries depending on the protein you are detecting
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated in immunoblot on colcemid treated human HeLa carcinoma cells
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
      Packaging Information
      Material Size200 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      -31567573156757
      Anti-phospho-Ser/Thr-Pro, MPM-2 (Mitotic protein monoclonal #2) - 24247612424761
      Anti-phospho-Ser/Thr-Pro, MPM-2 (Mitotic protein monoclonal #2) - 24525162452516
      Anti-phospho-Ser/Thr-Pro, MPM-2 (Mitotic protein monoclonal #2) - 1651616516
      Anti-phospho-Ser/Thr-Pro, MPM-2 (Mitotic protein monoclonal #2) - 1664816648
      Anti-phospho-Ser/Thr-Pro, MPM-2 (Mitotic protein monoclonal #2) - 1678116781
      Anti-phospho-Ser/Thr-Pro, MPM-2 (Mitotic protein monoclonal #2) - 1859418594
      Anti-phospho-Ser/Thr-Pro, MPM-2 (Mitotic protein monoclonal #2) - 19924431992443
      Anti-phospho-Ser/Thr-Pro, MPM-2 (Mitotic protein monoclonal #2) - 2075820758
      Anti-phospho-Ser/Thr-Pro, MPM-2 (Mitotic protein monoclonal #2) - 21166182116618

      References

      Reference overviewApplicationSpeciesPub Med ID
      Multiple assembly mechanisms anchor the KMN spindle checkpoint platform at human mitotic kinetochores.
      Kim, S; Yu, H
      The Journal of cell biology  208  181-96  2015

      Show Abstract
      25601404 25601404
      Dual targeting of microtubule and topoisomerase II by α-carboline derivative YCH337 for tumor proliferation and growth inhibition.
      Yi, JM; Zhang, XF; Huan, XJ; Song, SS; Wang, W; Tian, QT; Sun, YM; Chen, Y; Ding, J; Wang, YQ; Yang, CH; Miao, ZH
      Oncotarget  6  8960-73  2015

      Show Abstract
      25840421 25840421
      FBXW7 and USP7 regulate CCDC6 turnover during the cell cycle and affect cancer drugs susceptibility in NSCLC.
      Morra, F; Luise, C; Merolla, F; Poser, I; Visconti, R; Ilardi, G; Paladino, S; Inuzuka, H; Guggino, G; Monaco, R; Colecchia, D; Monaco, G; Cerrato, A; Chiariello, M; Denning, K; Claudio, PP; Staibano, S; Celetti, A
      Oncotarget  6  12697-709  2015

      Show Abstract
      25885523 25885523
      Orally active microtubule-targeting agent, MPT0B271, for the treatment of human non-small cell lung cancer, alone and in combination with erlotinib.
      Tsai, AC; Wang, CY; Liou, JP; Pai, HC; Hsiao, CJ; Chang, JY; Wang, JC; Teng, CM; Pan, SL
      Cell death & disease  5  e1162  2014

      Show Abstract
      24722287 24722287
      Protein kinase C β inhibition by enzastaurin leads to mitotic missegregation and preferential cytotoxicity toward colorectal cancer cells with chromosomal instability (CIN).
      Ouaret, D; Larsen, AK
      Cell cycle (Georgetown, Tex.)  13  2697-706  2014

      Show Abstract
      25486357 25486357
      Homeostatic control of polo-like kinase-1 engenders non-genetic heterogeneity in G2 checkpoint fidelity and timing.
      Liang, H; Esposito, A; De, S; Ber, S; Collin, P; Surana, U; Venkitaraman, AR
      Nature communications  5  4048  2014

      Show Abstract
      Immunofluorescence24893992 24893992
      Pin1-dependent signalling negatively affects GABAergic transmission by modulating neuroligin2/gephyrin interaction.
      Antonelli, R; Pizzarelli, R; Pedroni, A; Fritschy, JM; Del Sal, G; Cherubini, E; Zacchi, P
      Nature communications  5  5066  2014

      Show Abstract
      25297980 25297980
      Comparative analysis of mitosis-specific antibodies for bulk purification of mitotic populations by fluorescence-activated cell sorting.
      Campbell, Amy E, et al.
      BioTechniques, 56: 90-4 (2014)  2014

      Show Abstract
      Flow Cytometry24502799 24502799
      Identification of a mitotic death signature in cancer cell lines.
      Sakurikar, N; Eichhorn, JM; Alford, SE; Chambers, TC
      Cancer letters  343  232-8  2014

      Show Abstract
      24099917 24099917
      Adenovirus E4orf4 protein-induced death of p53-/- H1299 human cancer cells follows a G1 arrest of both tetraploid and diploid cells due to a failure to initiate DNA synthesis.
      Cabon, L; Sriskandarajah, N; Mui, MZ; Teodoro, JG; Blanchette, P; Branton, PE
      Journal of virology  87  13168-78  2013

      Show Abstract
      Western Blotting24067978 24067978

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies