A part of MilliporeSigma

ABS48 | Anti-phospho-SMAD3 (Ser213) Antibody

200 µg  
Retrieving price...
Price could not be retrieved
Minimum Quantity needs to be mulitiple of
Upon Order Completion More Information
You Saved ()
Request Pricing
Limited AvailabilityLimited Availability
Limited Quantities Available
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service

      Special Offers


      Contact Customer Service

      Click To Print This Page


      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, Ch WB Rb Affinity Purified Polyclonal Antibody
      Catalogue NumberABS48
      DescriptionAnti-phospho-SMAD3 (Ser213) Antibody
      Alternate Names
      • Mothers against decapentaplegic homolog 3
      • MAD homolog 3
      • Mad3
      • Mothers against DPP homolog 3
      • hMAD-3
      • JV15-2
      • SMAD family member 3
      • SMAD 3
      • Smad3
      • hSMAD3
      Background InformationMembers of the SMAD family of signal transduction molecules are components of a critical intracellular pathway that transmits TGF-β signals from the cell surface into the nucleus. Three distinct classes of SMADs have been defined: the receptor-regulated SMADs (R-SMADs), which include Smad1, 2, 3, 5 and 8, the common-mediator SMAD (co-SMAD), SMAD4, and the antagonistic or inhibitory SMADs (I-SMADs), SMAD6 and 7. Once in the nucleus, SMADs can target a variety of DNA binding proteins to regulate transcriptional responses. Following stimulation by TGF-β, SMAD2 and SMAD3 become phosphorylated at their carboxyl termini (Ser465 and 467 on SMAD2; Ser423 and 425 on SMAD3) by TGF-β Receptor I. Phosphorylated SMAD 2/3 can complex with SMAD4, translocate to the nucleus and regulate gene expression.
      Product Information
      FormatAffinity Purified
      • Untreated and TGF beta treated HaCat cell lysates
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      ApplicationAnti-phospho-SMAD3 (Ser213) Antibody detects level of phospho-SMAD3 (Ser213) & has been published & validated for use in Western Blotting.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to human SMAD3 phosphorylated at Ser213.
      EpitopePhosphorylated Ser213
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      SpecificityThis antibody recognizes SMAD3 phosphorylated at Ser213.
      Species Reactivity
      • Human
      • Chicken
      Species Reactivity NoteDemonstrated to react with Human. Predicted to react with Chicken based on 100% sequence homology. Other homologies: Bovine, Xenopus, and Zebrafish (90% sequence homology).
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • SMAD3
      • MADH3
      Purification MethodAffinity Purfied
      UniProt Number
      Molecular Weight~52 kDa observed. Variability may be observed in western blots due to post translational modification.
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in untreated and TGF beta treated HaCat cell lysates.

      Western Blot Analysis: 2 µg/mL of this antibody detected SMAD3 on 10 µg of untreated and TGF beta treated HaCat cell lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size200 µg
      Transport Information
      Supplemental Information

      Related Products & Applications

      Related Products

      Catalog Number Description  
      04-1035 Anti-SMAD3 Antibody, clone EP568Y, rabbit monoclonal Show Pricing & Availability
      04-953 Anti-phospho-Smad2 (Ser465/467) Antibody, clone A5S, rabbit monoclonal Show Pricing & Availability
      07-1389 Anti-phospho-Smad3 (Ser423/425) Antibody Show Pricing & Availability
      07-408 Anti-Smad2/3 Antibody Show Pricing & Availability
      AP182P Donkey Anti-Rabbit IgG Antibody, HRP conjugate, Species Adsorbed Show Pricing & Availability
      06-920 Anti-Smad3 Antibody Show Pricing & Availability
      ABS47 Anti-phospho-SMAD3 (Tyr179) Antibody Show Pricing & Availability
      MABT521 Anti-SMAD3, clone 5G11 Antibody Show Pricing & Availability

      View Gene Related Products for this Target


      Life Science Research > Antibodies and Assays > Primary Antibodies