07-2113 | Anti-phospho-Ribosomal Protein S6 Antibody (Ser240/Ser244)

07-2113
100 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, R, B, Xn WB, ICC Rb Affinity Purified Polyclonal Antibody
      Description
      Catalogue Number07-2113
      DescriptionAnti-phospho-Ribosomal Protein S6 Antibody (Ser240/Ser244)
      Alternate Names
      • 40S ribosomal protein S6
      • Phosphoprotein NP33
      Background InformationS6 (Ribosomal protein S6, RpS6, 40S ribosomal protein S6) is known to play a role in cell growth and proliferation. It lays downstream of both the PI3K/Akt/mTOR pathway as a substrate of p70 S6 kinase and the Ras/Erk pathway as a substrate of RSK. S6 is phosphorylated in response to stimulation by growth factors, tumor promoting agents, and mitogens. It is dephosphorylated at growth arrest. There are two major areas of S6 phosphorylation; Ser235/Ser236 that are known to be phosphorylated by both p70S6 Kinase as well as RSK and Ser240/244 which is thought to only be phosphorylated by p70S6K.
      References
      Product Information
      FormatAffinity Purified
      Control
      • PMA treated and untreated A431 cell lysates
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationThis phospho-Ribosomal Protein S6 antibody is validated for use in WB & ICC for the detection of the phospho-Ribosomal Protein S6 protein.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      Application NotesImmunocytochemistry Analysis: 1:500 dilution from a representative lot detected Ribosomal Protein S6 in A431 cells and PMA treated A431 cells.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to human Ribosomal Protein S6 phosphorylated at Ser240/Ser244.
      EpitopePhosphorylated Ser240/Ser244
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityThis antibody recognizes Ribosomal Protein S6 phosphorylated at Ser240/Ser244.
      Species Reactivity
      • Human
      • Mouse
      • Rat
      • Bovine
      • Xenopus
      Species Reactivity NoteDemonstrated to react with Human. Predicted to react with Mouse, Rat, Bovine, Xenopus, and Zebra Fish based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryRibosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a cytoplasmic ribosomal protein that is a component of the 40S subunit. The protein belongs to the S6E family of ribosomal proteins. It is the major substrate of protein kinases in the ribosome, with subsets of five C-terminal serine residues phosphorylated by different protein kinases. Phosphorylation is induced by a wide range of stimuli, including growth factors, tumor-promoting agents, and mitogens. Dephosphorylation occurs at growth arrest. The protein may contribute to the control of cell growth and proliferation through the selective translation of particular classes of mRNA. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq].
      Gene Symbol
      • RPS6
      Modifications
      • Phosphorylation
      • Phosphorylation
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFUNCTION: May play an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA.

      PTM: Ribosomal protein S6 is the major substrate of protein kinases in eukaryote ribosomes. The phosphorylation is stimulated by growth factors, tumor promoting agents, and mitogens. It is dephosphorylated at growth arrest.

      SEQUENCE SIMILARITIES: Belongs to the ribosomal protein S6e family.
      Molecular Weight ~32 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in PMA treated and untreated A431 cell lysates.

      Western Blot Analysis: 2 µg/mL of this antibody detected Ribosomal Protein S6 on 10 µg of PMA treated and untreated A431 cell lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-phospho-Ribosomal Protein S6 (Ser240/Ser244) - 2420516 2420516
      Anti-phospho-Ribosomal Protein S6 (Ser240/Ser244) - 1962106 1962106
      Anti-phospho-Ribosomal Protein S6 (Ser240/Ser244) - 2172768 2172768
      Anti-phospho-Ribosomal Protein S6 (Ser240/Ser244) - 2201513 2201513
      Anti-phospho-Ribosomal Protein S6 (Ser240/Ser244) - 2283575 2283575
      Anti-phospho-Ribosomal Protein S6 (Ser240/Ser244) - NRG1872322 NRG1872322
      Anti-phospho-Ribosomal Protein S6 (Ser240/Ser244) -2567148 2567148
      Anti-phospho-Ribosomal Protein S6 (Ser240/Ser244) -2649263 2649263
      Anti-phospho-Ribosomal Protein S6 (Ser240/Ser244) -2702111 2702111
      Anti-phospho-Ribosomal Protein S6 -2545766 2545766

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies