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07-811 | Anti-phospho-Raf1 (Ser259) Antibody

100 µL  
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      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H WB Rb Affinity Purified Polyclonal Antibody
      Catalogue Number07-811
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-phospho-Raf1 (Ser259) Antibody
      Alternate Names
      • CRAF
      • Raf-1
      • c-Raf
      Product Information
      FormatAffinity Purified
      Presentation100 μL of affinity purified rabbit polyclonal IgG in 50% storage buffer (PBS (without Mg2+ and Ca2+), pH 7.3 containing 1.0 mg/mL BSA (IgG, protease free) and 0.05% sodium azide).
      ApplicationThis Anti-phospho-Raf1 (Ser259) Antibody is validated for use in WB for the detection of phospho-Raf1 (Ser259).
      Key Applications
      • Western Blotting
      Application NotesImmunoblot Analysis: A 0.50μg/mL dilution of this lot detected phospho-c-Raf (Ser259) in RIPA lysate of EGF treated HEK293 cells.
      Biological Information
      ImmunogenPeptide corresponding to amino acid region encompassing the human, mouse, and rat phospho-Raf1 (Ser259).
      SpecificityRaf1 phosphorylated on serine residue 259
      Species Reactivity
      • Human
      Species Reactivity NotePredicted to cross-react with mouse and rat based on sequence similarity(100%), but have not been tested.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryRaf-1 is a MAP kinase kinase kinase (MAP3K) which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated Raf-1 can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2 which in turn phosphorylate to activate the serine/threonine specific protein kinases ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. [Contributed text]
      Gene Symbol
      • RAF1
      • C-RAF
      • cRaf
      • Raf-1
      • c-Raf
      • CRAF
      • RAF
      • Phosphorylation
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P04049 # Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. Part of the Ras-dependent signaling pathway from receptors to the nucleus. Protects cells from apoptosis mediated by STK3.
      COFACTOR: Binds 2 zinc ions per subunit.
      SIZE: 648 amino acids; 73052 Da
      SUBUNIT: Interacts with Ras proteins. The interaction with Ras is antagonized by RIN1. Weakly interacts with RIT1 (By similarity). Interacts with STK3, which inhibits its pro-apoptotic activity.
      PTM: Phosphorylated upon DNA damage, probably by ATM or ATR.SIMILARITY:SwissProt: P04049 ## Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. RAF subfamily. & Contains 1 phorbol-ester/DAG-type zinc finger. & Contains 1 protein kinase domain. & Contains 1 RBD (Ras-binding) domain.
      Molecular Weight74 kDa
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by immunoblot.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 2 years at -80°C from date of shipment. Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -80°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information

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      Life Science Research > Antibodies and Assays > Primary Antibodies