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07-052 | Anti-phospho-Phospholamban (Ser16) Antibody

100 µg  
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      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, R, H, B, Ca, Po, Rb WB Rb Affinity Purified Polyclonal Antibody
      Catalogue Number07-052
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-phospho-Phospholamban (Ser16) Antibody
      Alternate Names
      • cardiac phospholamban
      Background InformationPhospholamban (PLB) is a 52 amino acid phosphoprotein which regulates the calcium pump of cardiac sarcoplasmic reticulum (SR). PLB is an oligomer of five identical subunits each having a cytoplasmic and transmembrane domain. The cytoplasmic domain (residues 1 to 25) contains the phosphorylation sites and is highly basic and readily cleaved by proteases; whereas the transmembrane domain (residues 25 to 52) is mostly hydrophobic, protease resistant and stabilizes the pentamer. Following adrenergic stimulation of cardiac muscle, PLB is phosphorylated at Ser16 and at Thr17 which is correlated with stimulation of calcium transport activity across the SR membrane and relaxation of cardiac fibers.
      Product Information
      FormatAffinity Purified
      • Bovine cardiac preparation, treated with PKA.
      PresentationPurified rabbit polyclonal IgG in buffer containing 0.014 M phosphate buffer, pH 7.6, 0.175 M NaCl, 0.07% sodium azide and 30% glycerol. Store at -20°C.
      ApplicationThis Anti-phospho-Phospholamban (Ser16) Antibody is validated for use in WB for the detection of phospho-Phospholamban (Ser16).
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenKLH-conjugated, synthetic peptide corresponding to amino acids 14-25 of human cardiac phospholamban (RA[pS]TIEMPQQAR-C) with an added C-terminal cysteine. The immunizing sequence is identical in mouse, rat, rabbit, pig and dog.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      SpecificityRecognizes phosphorylated phospho-lamban (Ser16), Mr 5-25 kDa. Additional non-specific bands may be detected, Mr 75 & 100 kDa, in some lysates.
      Species Reactivity
      • Mouse
      • Rat
      • Human
      • Bovine
      • Canine
      • Pig
      • Rabbit
      Species Reactivity NoteBovine. Predicted to cross-react with human, mouse, rat, rabbit, pig and dog.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is found as a pentamer and is a major substrate for the cAMP-dependent protein kinase in cardiac muscle. The encoded protein is an inhibitor of cardiac muscle sarcoplasmic reticulum Ca(2+)-ATPase in the unphosphorylated state, but inhibition is relieved upon phosphorylation of the protein. The subsequent activation of the Ca(2+) pump leads to enhanced muscle relaxation rates, thereby contributing to the inotropic response elicited in heart by beta-agonists. The encoded protein is a key regulator of cardiac diastolic function. Mutations in this gene are a cause of inherited human dilated cardiomyopathy with refractory congestive heart failure.
      Gene Symbol
      • CMD1P
      • OTTHUMP00000017092
      • PLB
      • phospholamban
      • Phosphorylation
      Purification MethodImmunoaffinity Chromatography
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P26678 # Phospholamban has been postulated to regulate the activity of the calcium pump of cardiac sarcoplasmic reticulum.
      SIZE: 52 amino acids; 6109 Da
      SUBUNIT: Homopentamer.
      PTM: Phosphorylated in response to beta-adrenergic stimulation.
      DISEASE:SwissProt: P26678 # Defects in PLN are the cause of dilated cardiomyopathy 1P (CMD1P) [MIM:609909]. Cardiomyopathy is a disorder characterized by cardiac dilation and reduced systolic function.
      SIMILARITY: SwissProt: P26678 ## Belongs to the phospholamban family.
      Molecular WeightVaries
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by western blot on bovine cardiac preparation that was incubated with PKA.

      Western Blot Analysis:
      0.5-2 µg/mL of this lot detected phosphorylated phospholamban from a bovine cardiac preparation that was incubated with 10 µg PKA (Catalog # 14-114) for 10 minutes.
      Boiling may reduce the 25 kDa pentamer into tri-, di- or monomers. In addition, phosphorylation may disrupt the pentamer into dimers and monomers.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-phospho-Phospholamban (Ser16) 2476983
      Anti-phospho-Phospholamban (Ser16) (rabbit immunoaffinity purified IgG) - DAM1437130 DAM1437130
      Anti-phospho-Phospholamban (Ser16) (rabbit immunoaffinity purified IgG) - JBC1945053 JBC1945053
      Anti-phospho-Phospholamban (Ser16) - 2430488 2430488
      Anti-phospho-Phospholamban (Ser16) - 20066 20066
      Anti-phospho-Phospholamban (Ser16) - 2016033 2016033
      Anti-phospho-Phospholamban (Ser16) - 2073779 2073779
      Anti-phospho-Phospholamban (Ser16) - 22605 22605
      Anti-phospho-Phospholamban (Ser16) - 2266820 2266820
      Anti-phospho-Phospholamban (Ser16) - 23541 23541
      Anti-phospho-Phospholamban (Ser16) - 29711 29711
      Anti-phospho-Phospholamban (Ser16) - 32366 32366
      Anti-phospho-Phospholamban (Ser16) - DAM1437130 DAM1437130
      Anti-phospho-Phospholamban (Ser16) - DAM1810817 DAM1810817
      Anti-phospho-Phospholamban (Ser16) - JBC1863328 JBC1863328
      Anti-phospho-Phospholamban (Ser16) -2571186 2571186
      Anti-phospho-Phospholamban (Ser16) -2605376 2605376
      Anti-phospho-Phospholamban (Ser16) -2712330 2712330
      Anti-phospho-Phospholamban (Ser16) -2766475 2766475

      References | 26 Available | See All References

      Reference overviewApplicationPub Med ID
      Atrial fibrillation complicated by heart failure induces distinct remodeling of calcium cycling proteins.
      Lugenbiel, P; Wenz, F; Govorov, K; Schweizer, PA; Katus, HA; Thomas, D
      PloS one 10 e0116395 2015

      Show Abstract
      25775120 25775120
      Long term ablation of protein kinase A (PKA)-mediated cardiac troponin I phosphorylation leads to excitation-contraction uncoupling and diastolic dysfunction in a knock-in mouse model of hypertrophic cardiomyopathy.
      Dweck, D; Sanchez-Gonzalez, MA; Chang, AN; Dulce, RA; Badger, CD; Koutnik, AP; Ruiz, EL; Griffin, B; Liang, J; Kabbaj, M; Fincham, FD; Hare, JM; Overton, JM; Pinto, JR
      The Journal of biological chemistry 289 23097-111 2014

      Show Abstract
      Western Blotting24973218 24973218
      Pak1 is required to maintain ventricular Ca²⁺ homeostasis and electrophysiological stability through SERCA2a regulation in mice.
      Wang, Y; Tsui, H; Ke, Y; Shi, Y; Li, Y; Davies, L; Cartwright, EJ; Venetucci, L; Zhang, H; Terrar, DA; Huang, CL; Solaro, RJ; Wang, X; Lei, M
      Circulation. Arrhythmia and electrophysiology 7 938-48 2014

      Show Abstract
      25217043 25217043
      Noncanonical EF-hand motif strategically delays Ca2+ buffering to enhance cardiac performance.
      Wang, W; Barnabei, MS; Asp, ML; Heinis, FI; Arden, E; Davis, J; Braunlin, E; Li, Q; Davis, JP; Potter, JD; Metzger, JM
      Nature medicine 19 305-12 2013

      Show Abstract
      23396207 23396207
      Lifelong exposure to bisphenol a alters cardiac structure/function, protein expression, and DNA methylation in adult mice.
      Patel, BB; Raad, M; Sebag, IA; Chalifour, LE
      Toxicological sciences : an official journal of the Society of Toxicology 133 174-85 2013

      Show Abstract
      23418087 23418087
      Impaired contractile function due to decreased cardiac myosin binding protein C content in the sarcomere.
      Cheng, Y; Wan, X; McElfresh, TA; Chen, X; Gresham, KS; Rosenbaum, DS; Chandler, MP; Stelzer, JE
      American journal of physiology. Heart and circulatory physiology 305 H52-65 2013

      Show Abstract
      23666674 23666674
      The effects of neuregulin on cardiac Myosin light chain kinase gene-ablated hearts.
      Chang, AN; Huang, J; Battiprolu, PK; Hill, JA; Kamm, KE; Stull, JT
      PloS one 8 e66720 2013

      Show Abstract
      23776695 23776695
      Anthrax lethal toxin induces acute diastolic dysfunction in rats through disruption of the phospholamban signaling network.
      Golden, HB; Watson, LE; Nizamutdinov, D; Feng, H; Gerilechaogetu, F; Lal, H; Verma, SK; Mukhopadhyay, S; Foster, DM; Dillmann, WH; Dostal, DE
      International journal of cardiology 168 3884-95 2013

      Show Abstract
      23907041 23907041
      SERCA Cys674 sulphonylation and inhibition of L-type Ca2+ influx contribute to cardiac dysfunction in endotoxemic mice, independent of cGMP synthesis.
      Hobai, IA; Buys, ES; Morse, JC; Edgecomb, J; Weiss, EH; Armoundas, AA; Hou, X; Khandelwal, AR; Siwik, DA; Brouckaert, P; Cohen, RA; Colucci, WS
      American journal of physiology. Heart and circulatory physiology 305 H1189-200 2013

      Show Abstract
      23934853 23934853
      Myocardial ATGL overexpression decreases the reliance on fatty acid oxidation and protects against pressure overload-induced cardiac dysfunction.
      Kienesberger, PC; Pulinilkunnil, T; Sung, MM; Nagendran, J; Haemmerle, G; Kershaw, EE; Young, ME; Light, PE; Oudit, GY; Zechner, R; Dyck, JR
      Molecular and cellular biology 32 740-50 2012

      Show Abstract
      22158969 22158969

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