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ABS204 | Anti-phospho-PDHE1-A type I (Ser293) Antibody

ABS204
50 µL  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, R, M, B, Pm WB, ICC, IP Rb Affinity Purified Polyclonal Antibody
      Description
      Catalogue NumberABS204
      DescriptionAnti-phospho-PDHE1-A type I (Ser293) Antibody
      Alternate Names
      • Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial
      • PDHE1-A type I
      Background InformationIn many organisms, the pyruvate dehydrogenase (PDH) complex catalyzes the overall, irreversible conversion of pyruvate to acetyl-CoA and CO2 in the aerobic pathway. This complex also serves as a key regulator for cardiac substrate selection. It contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1 or PDHE1-A type I), dihydrolipoamide acetyltransferase (E2), and lipoamide dehydrogenase (E3). PDH is regulated by both pyruvate dehydrogenase kinase (PDK)-mediated phosphorylation and feedback inhibition.
      References
      Product Information
      FormatAffinity Purified
      Control
      • Untreated and DCA-treated HEK293 cell lysates
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationDetect phospho-PDHE1-A type I (Ser293) using this Anti-phospho-PDHE1-A type I (Ser293) Antibody validated for use in WB, IC & IP.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      • Immunoprecipitation
      Application NotesImmunocytochemistry Analysis: A 1:500 dilution of this antibody from a representative lot detected phospho-PDHE1-A type I (Ser293) in untreated and DCA-treated HEK293 cells.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to human PDHE1-A type I phosphorylated at Ser293.
      EpitopePhosphorylated Ser293
      HostRabbit
      SpecificityThis antibody recognizes PDHE1-A type I phosphorylated at Ser293.
      Species Reactivity
      • Human
      • Rat
      • Mouse
      • Bovine
      • Primate
      Species Reactivity NoteDemonstrated to react with Human.
      Predicted to react with Rat, Mouse, Bovine, Zebra Fish, and Primate based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe pyruvate dehydrogenase (PDH) complex is a nuclear-encoded mitochondrial multienzyme complex that catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle. The PDH complex is composed of multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3). The E1 enzyme is a heterotetramer of two alpha and two beta subunits. This gene encodes the E1 alpha 1 subunit containing the E1 active site, and plays a key role in the function of the PDH complex. Mutations in this gene are associated with pyruvate dehydrogenase E1-alpha deficiency and X-linked Leigh syndrome. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
      Gene Symbol
      • PDHA1
      • PHE1A
      Modifications
      • Phosphorylation
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFUNCTION: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO2. It contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3).

      CATALYTIC ACTIVITY: Pyruvate + [dihydrolipoyllysine-residue acetyltransferase] lipoyllysine = [dihydrolipoyllysine-residue acetyltransferase] S-acetyldihydrolipoyllysine + CO2.

      COFACTOR: Thiamine pyrophosphate.

      ENZYME REGULATION: E1 activity is regulated by phosphorylation (inactivation) and dephosphorylation (activation) of the alpha subunit.

      SUBUNIT STRUCTURE: Tetramer of 2 alpha and 2 beta subunits.

      SUBCELLULAR LOCATION: Mitochondrion matrix.

      TISSUE SPECIFICITY: Ubiquitous.
      Molecular Weight~43 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in DCA untreated and treated HEK293 cell lysates.

      Western Blot Analysis: A 1:10,000 dilution of this antibody detected phospho-PDHE1-A type I (Ser293) in untreated and DCA-treated HEK293 cell lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size50 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-phospho-PDHE1-A type I (Ser293) - 2343142 2343142
      Anti-phospho-PDHE1-A type I (Ser293) - 2384762 2384762
      Anti-phospho-PDHE1-A type I (Ser293) - 2438685 2438685
      Anti-phospho-PDHE1-A type I (Ser293) - 2465296 2465296
      Anti-phospho-PDHE1-A type I (Ser293) - 2033663 2033663
      Anti-phospho-PDHE1-A type I (Ser293) - 2219396 2219396
      Anti-phospho-PDHE1-A type I (Ser293) - 2266776 2266776
      Anti-phospho-PDHE1-A type I (Ser293) - 2315725 2315725
      Anti-phospho-PDHE1-A type I (Ser293) - NRG1953331 NRG1953331
      Anti-phospho-PDHE1-A type I (Ser293) -2491250 2491250
      Anti-phospho-PDHE1-A type I (Ser293) -2510407 2510407
      Anti-phospho-PDHE1-A type I (Ser293) -2581365 2581365
      Anti-phospho-PDHE1-A type I (Ser293) -2619689 2619689
      Anti-phospho-PDHE1-A type I (Ser293) -2649271 2649271
      Anti-phospho-PDHE1-A type I (Ser293) -2686120 2686120
      Anti-phospho-PDHE1-A type I (Ser293) -2719453 2719453
      Anti-phospho-PDHE1-A type I (Ser293) -2768440 2768440
      Anti-phospho-PDHE1-A type I -2806544 2806544

      References

      Reference overviewApplicationPub Med ID
      c-Myc Alters Substrate Utilization and O-GlcNAc Protein Posttranslational Modifications without Altering Cardiac Function during Early Aortic Constriction.
      Ledee, D; Smith, L; Bruce, M; Kajimoto, M; Isern, N; Portman, MA; Olson, AK
      PloS one 10 e0135262 2015

      Show Abstract
      Western Blotting26266538 26266538
      Propofol compared with isoflurane inhibits mitochondrial metabolism in immature swine cerebral cortex.
      Kajimoto, M; Atkinson, DB; Ledee, DR; Kayser, EB; Morgan, PG; Sedensky, MM; Isern, NG; Des Rosiers, C; Portman, MA
      Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism 34 514-21 2014

      Show Abstract
      24398942 24398942

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies