ABS160 | Anti-phospho-MKK4 Antibody (Ser257/Thr261)

ABS160
100 µL  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, R, B, Xn, Zebrafish WB, PIA Rb Affinity Purified Polyclonal Antibody
      Description
      Catalogue NumberABS160
      Replaces07-857
      DescriptionAnti-phospho-MKK4 Antibody (Ser257/Thr261)
      Alternate Names
      • Dual specificity mitogen-activated protein kinase kinase 4
      • MAP kinase kinase 4
      • MAPKK 4
      • JNK-activating kinase 1
        MAPK/ERK kinase 4
      • MEK 4
      • SAPK/ERK kinase 1
      • SEK1
      • Stress-activated protein kinase kinase 1
      • SAPK kinase 1
      • SAPKK-1
      • SAPKK1
      • c-Jun N-terminal kinase kinase 1
      • JNKK
      Background InformationAlso known as dual specificity mitogen-activated protein kinase kinase 4, JNK-activating kinase 1, and MAP/ERK kinase 4 among other names, MKK4 is protein kinase that plays a critical role in the MAP kinase signal transduction pathway and in stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling. MKK4 is required for maintenance of peripheral lymphoid homeostasis, and is involved in stress response and apoptosis. It also acts as a metastasis suppressor in certain cancers, such as prostate and ovarian cancer.
      References
      Product Information
      FormatAffinity Purified
      Control
      • Activated MKK4 (Cat. No. 14-377) alone, or with non phospho or phospho peptide
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationDetect the phospho-MKK4 (Ser257/Thr261) protein using this Anti-phospho-MKK4 (Ser257/Thr261) validated for use in WB & Peptide Inhibition Assay.
      Key Applications
      • Western Blotting
      • Peptide Inhibition Assay
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to human MKK4 phosphorylated at Ser257 and Thr261.
      EpitopePhosphorylated at Ser257 and Thr261
      HostRabbit
      SpecificityThis antibody recognizes MKK4 phosphorylated at Ser257 and phosphorylated at Thr261.
      Species Reactivity
      • Human
      • Mouse
      • Rat
      • Bovine
      • Xenopus
      • Zebrafish
      Species Reactivity NoteDemonstrated to react with Human. Predicted to react with Mouse, Rat, Bovine, Xenopus, and Zebrafish based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • MAP2K4
      • JNKK1
      • MEK4
      • MKK4
      • PRKMK4
      • SEK1
      • SERK1
      • SKK1
      Purification MethodAffinity Purfied
      UniProt Number
      Molecular Weight~64 kDa observed. The calculated molecular weight of MKK4 is 44 kDa, but the GST-tagged MKK4 protein used in this Western Blot is observed at ~64 kDa.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in activated MKK4 (Cat. No. 14-377) alone, or with non phospho or phospho peptide.

      Western Blot Analysis: A 1:30,000 diluiton of this antibody detected phospho-MKK4 (Ser257/Thr261) in 500 ng of activated MKK4 recombinant protein. Peptide inhibition was observed with the addition of phospho peptides, but not with the addition of non-phospho peptides or antibody alone (500 ng of recombinant protein was loaded onto the SDS-PAGE gel. It is recommended for end-users to determine optimal antibody dilutions respective to the amount of test sample used.).
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Related Products & Applications

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      Catalog Number Description  
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      07-857 Anti-phospho-MKK4 (Ser257/Thr261) Antibody Show Pricing & Availability
      MABS814 Anti-MAP2K4, clone 5H4 Antibody Show Pricing & Availability

      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies