MABE76 Anti-phospho-Histone H3 (Ser28) Antibody, clone HTA28

MABE76
100 µL  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H WB, DB, ICC R Purified Monoclonal Antibody
      Description
      Catalogue NumberMABE76
      DescriptionAnti-phospho-Histone H3 (Ser28) Antibody, clone HTA28
      Alternate Names
      • Histone H3.3
      • H3F3A
      • H3.3A
      • H3F3
      • H3F3B
      • H3S28P
      Background InformationHistones are highly conserved proteins that serve as the structural scaffold for the organization of nuclear DNA into chromatin. The four core histones, H2A, H2B, H3, and H4, assemble into an octamer containing 2 molecules of each type of histone. Histones are modified post-translationally by the actions of enzymes in both the nucleus and cytoplasm. These modifications, which include acetylation, phosphorylation, methylation, and ubiquitin-ation, occur predominantly on the N-terminal and C-terminal tails that extend beyond the nucleosome core particle. Phosphorylation at Ser10, Ser28 and Thr11 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis.
      References
      Product Information
      FormatPurified
      Control
      • Untreated and colcemid treated HeLa acid extract.
      PresentationPurified rat monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-phospho-Histone H3 (Ser28) Antibody, clone HTA28 is a Rat Monoclonal Antibody for detection of phospho-Histone H3 (Ser28) also known as H3F3, H3F3B, H3S28P & has been validated in WB, DB, ICC.
      Key Applications
      • Western Blotting
      • Dot Blot
      • Immunocytochemistry
      Application NotesDot Blot Specificity Analysis: A representative lot detected peptides corresponding to phosphorylated-Histone H3 Ser28, but did not detect unmodified peptides against H3 Ser28 or other peptides corresponding to additional histone modification sites.

      Immunocytochemistry Analysis: A representative lot from an independent laboratory detected phosphorylated-Histone H3 in U251, HeLa, NIH/3T3, MDBK, BHK, and tsBN2 cells. (Goto, H., et al. (1999). J Biol Chem. 274(36):25543-25549.)
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to human Histone H3 phosphorylated at Ser28.
      EpitopePhosphorylated Ser28.
      Cloneclone HTA28
      HostRat
      SpecificityThis antibody recognizes Histone H3 phosphorylated at Ser28.
      IsotypeIgG2aκ
      Species Reactivity
      • Human
      Species Reactivity NoteDemonstrated to react with Human. Predicted to react with multiple vertebrate species based on 100% sequence homology.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryHistones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene contains introns and its mRNA is polyadenylated, unlike most histone genes. The protein encoded is a replication-independent member of the histone H3 family.
      Gene Symbol
      • H3F3A
      • H3.3A
      • H3F3
      • PP781
      • H3F3B
      • H3.3B
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: Variant histone H3 which replaces conventional H3 in a wide range of nucleosomes in active genes. Constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. Deposited at sites of nucleosomal displacement throughout transcribed genes, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

      SUBUNIT STRUCTURE: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. Interacts with HIRA, a chaperone required for its incorporation into nucleosomes.

      SUBCELLULAR LOCATION: Nucleus. Chromosome.
      Molecular Weight~15 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in untreated and colcemid treated HeLa acid extract.

      Western Blot Analysis: A 1:50,000 dilution of this antibody detected phosphorylated-Histone H3 in 10 µg of untreated and colcemid treated Histone H3.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-phospho-Histone H3 (Ser28) Antibody, clone HTA28 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-phospho-Histone H3 (Ser28) Antibody, clone HTA28 Certificates of Analysis

      TitleLot Number
      Anti-phospho-Histone H3 (Ser28), clone HTA28 - 22943492294349
      Anti-phospho-Histone H3 (Ser28), clone HTA28 - 23325862332586
      Anti-phospho-Histone H3 (Ser28), clone HTA28 - Q1966979Q1966979
      Anti-phospho-Histone H3 (Ser28), clone HTA28 -25275812527581
      Anti-phospho-Histone H3 (Ser28), clone HTA28 Monoclonal Antibody2988819
      Anti-phospho-Histone H3 (Ser28), clone HTA28 clone HTA28-28317602831760

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      Life Science Research > Antibodies and Assays > Primary Antibodies