05-806 Anti-phospho-Histone H3 (Ser10) Antibody, clone 3H10

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      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H FC, ICC, IF, PIA, WB, Mplex M Purified Monoclonal Antibody
      Catalogue Number05-806
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-phospho-Histone H3 (Ser10) Antibody, clone 3H10
      Alternate Names
      • H3 histone, family 3A
      • H3S10P
      • Histone H3 (phospho S10)
      • H3 histone, family 3A
      • H3 histone, family 3B
      • H3 histone, family 3B (H3.3B)
      Background InformationHistone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.
      Product Information
      • UV-treated 293 cell extracts, UV-treated HeLa cell extracts, breast cancer tissue, HEPG2 cell extracts.
      PresentationPurified mouse IgG1k in buffer containing 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl and 0.05% sodium azide.
      ApplicationAnti-phospho-Histone H3 (Ser10) Antibody, clone 3H10 is a mouse monoclonal antibody for detection of Histone H3 phosphorylated at serine 10. This purified mAb, also known as H3S10p, is published in peer reviewed journals and has been validated in FC, ICC, IF, PIA, WB, Mplex.
      Key Applications
      • Flow Cytometry
      • Immunocytochemistry
      • Immunofluorescence
      • Peptide Inhibition Assay
      • Western Blotting
      • Multiplexing
      Application NotesFlow Cytometry:
      This antibody has been reported by an independent laboratory to detect phosphorylated histone H3 using flow cytometry.

      Beadlyte® Histone-Peptide Specificity Assay:
      1:1,000 to 1:81,000 dilutions of a previous lot were incubated with histone H3 peptides containing various modifications conjugated to Luminex® microspheres. (Figure B). Only the peptide containing phospho-serine 10 was detected.

      0.2 μg/mL of a previous lot showed positive chromosome immunostaining for mitotic A431 and HeLa cells fixed with 95% ethanol and 5% acetic acid and permeabilized with 0.1% Triton® X-100.

      Peptide Inhibition Analysis:
      Detection of histone H3 in immunoblots of colcemid treated HeLa acid extracts by anti-phospho-Histone H3 (Ser10) was diminished by 10 μM of histone H3 peptide containing phospho-serine 10, but not by peptides containing phospho-serine 28 or an unmodified histone H3 sequence (Figure C).

      Biological Information
      ImmunogenA proprietary immunogen based on a peptide sequence containing phospho-serine corresponding to residue 10 of human histone H3.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      SpecificityHistone H3 phosphorylated at Ser10.
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Broad species cross-reactivity is expected.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryHistones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
      Gene Symbol
      • HIST1H3A
      • H3FA
      • HIST1H3B
      • H3FL
      • HIST1H3C
      • H3FC
      • HIST1H3D
      • H3FB
      • HIST1H3E
      • H3FD
      • HIST1H3F
      • H3FI
      • HIST1H3G
      • H3FH
      • HIST1H3H
      • H3FK
      • HIST1H3I
      • H3FF
      • HIST1H3J
      • H3FJ
      • HIST3H3
      • H3FT
      • H3T
      • MGC126886
      • Phosphorylation
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q16695 # Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
      SIZE: 136 amino acids; 15508 Da
      SUBUNIT: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.
      TISSUE SPECIFICITY: Expressed in testicular cells.
      DEVELOPMENTAL STAGE: Expressed during S phase, then expression strongly decreases as cell division slows down during the process of differentiation.
      PTM: Acetylation is generally linked to gene activation. Acetylation on Lys-10 impairs methylation at Arg-9. Acetylation on Lys-19 and Lys-24 favors methylation at Arg-18 (By similarity). & Citrullination at Arg-9 and/or Arg-18 by PADI4 impairs methylation and represses transcription (By similarity). & Asymmetric dimethylation at Arg-18 by CARM1 is linked to gene activation. Symmetric dimethylation at Arg-9 by PRMT5 is linked to gene repression (By similarity). & Methylation at Lys-5, Lys-37 and Lys-80 are linked to gene activation. Methylation at Lys-5 facilitates subsequent acetylation of H3 and H4. Methylation at Lys-80 is associated with DNA double-strand break (DSB) responses and is a specific target for TP53BP1. Methylation at Lys-10 and Lys-28 are linked to gene repression. Methylation at Lys-10 is a specific target for HP1 proteins (CBX1, CBX3 and CBX5) and prevents subsequent phosphorylation at Ser-11 and acetylation of H3 and H4. Methylation at Lys-5 and Lys-80 require preliminary monoubiquitination of H2B at 'Lys-120'. Methylation at Lys-10 and Lys-28 are enriched in inactive X chromosome chromatin (By similarity). & Phosphorylated at Thr-4 by GSG2/haspin during prophase and dephosphorylated during anaphase. At centromeres, specifically phosphorylated at Thr-12 from prophase to early anaphase. Phosphorylated at Ser-11 during the whole mitosis. Phosphorylation at Ser-11, which is linked to gene activation, prevents methylation at Lys-10 but facilitates acetylation of H3 and H4. Phosphorylated at Ser-29 by MLTK isoform 1, RPS6KA5 or AURKB during mitosis or upon ultraviolet B irradiation (By similarity). & Phosphorylation at 'Ser-11' is crucial for chromosome condensation and cell-cycle progression during mitosis and meiosis. In addition phosphorylation at 'Ser-11' is important during interphase because it enables the transcription of genes following external stimulation, like stress or growth factors. Phosphorylation at 'Ser-11' is also an essential regulatory mechanism for neoplastic cell transformation. Phosphorylation at 'Ser-11' by AURKB/Aurora-B mediates the dissociation of HP1 proteins (CBX1, CBX3 and CBX5) from heterochromatin. & Ubiquitinated (By similarity).
      SIMILARITY: SwissProt: Q16695 ## Belongs to the histone H3 family.
      Molecular Weight17 kDa
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by western blot acid extracted proteins from mitotic HeLa cells treated with colcemid (Catalog # 17-306).

      Western Blot Analysis:
      0.05-0.5 μg/mL of this lot detected phosphorylated histone H3 in acid extracted proteins from mitotic HeLa cells treated with colcemid (Catalog # 17-306), but not unmodified recombinant Histone H3 (Catalog # 14-494) (Figure A).
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2 to 8°C from date of receipt.

      Handling Recommendations:
      Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information


      Anti-phospho-Histone H3 (Ser10) Antibody, clone 3H10 SDS


      Safety Data Sheet (SDS) 

      Anti-phospho-Histone H3 (Ser10) Antibody, clone 3H10 Certificates of Analysis

      TitleLot Number
      Anti-phospho-Histone H3 (Ser10), -28148002814800
      Anti-phospho-Histone H3 (Ser10), clone 3H10 - 21370792137079
      Anti-phospho-Histone H3 (Ser10), clone 3H10 - 23878252387825
      Anti-phospho-Histone H3 (Ser10), clone 3H10 - 06090408250609040825
      Anti-phospho-Histone H3 (Ser10), clone 3H10 - 13506241350624
      Anti-phospho-Histone H3 (Ser10), clone 3H10 - 19924481992448
      Anti-phospho-Histone H3 (Ser10), clone 3H10 - 20645142064514
      Anti-phospho-Histone H3 (Ser10), clone 3H10 - 22756172275617
      Anti-phospho-Histone H3 (Ser10), clone 3H10 - 25549002554900
      Anti-phospho-Histone H3 (Ser10), clone 3H10 - 2692326923


      Reference overviewApplicationPub Med ID
      Spindle assembly checkpoint acquisition at the mid-blastula transition.
      Zhang, M; Kothari, P; Lampson, MA
      PloS one  10  e0119285  2015

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      Immunofluorescence25741707 25741707
      Genetic manipulation of reptilian embryos: toward an understanding of cortical development and evolution.
      Nomura, T; Yamashita, W; Gotoh, H; Ono, K
      Frontiers in neuroscience  9  45  2015

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      25759636 25759636
      Selective disruption of aurora C kinase reveals distinct functions from aurora B kinase during meiosis in mouse oocytes.
      Balboula, AZ; Schindler, K
      PLoS genetics  10  e1004194  2014

      Show Abstract
      24586209 24586209
      An increase in MECP2 dosage impairs neural tube formation.
      Petazzi, P; Akizu, N; García, A; Estarás, C; Martínez de Paz, A; Rodríguez-Paredes, M; Martínez-Balbás, MA; Huertas, D; Esteller, M
      Neurobiology of disease  67  49-56  2014

      Show Abstract
      24657916 24657916
      Phosphoinositide 3-kinase alpha-dependent regulation of branching morphogenesis in murine embryonic lung: evidence for a role in determining morphogenic properties of FGF7.
      Carter, E; Miron-Buchacra, G; Goldoni, S; Danahay, H; Westwick, J; Watson, ML; Tosh, D; Ward, SG
      PloS one  9  e113555  2014

      Show Abstract
      25460003 25460003
      Rac1-dependent recruitment of PAK2 to G2 phase centrosomes and their roles in the regulation of mitotic entry.
      May, M; Schelle, I; Brakebusch, C; Rottner, K; Genth, H
      Cell cycle (Georgetown, Tex.)  13  2211-21  2014

      Show Abstract
      24840740 24840740
      TGF-β mediates early angiogenesis and latent fibrosis in an Emilin1-deficient mouse model of aortic valve disease.
      Munjal, C; Opoka, AM; Osinska, H; James, JF; Bressan, GM; Hinton, RB
      Disease models & mechanisms  7  987-96  2014

      Show Abstract
      Immunohistochemistry25056700 25056700
      CENP-A is essential for cardiac progenitor cell proliferation.
      McGregor, M; Hariharan, N; Joyo, AY; Margolis, RL; Sussman, MA
      Cell cycle (Georgetown, Tex.)  13  739-48  2014

      Show Abstract
      Immunohistochemistry24362315 24362315
      Adenovirus E4orf4 protein-induced death of p53-/- H1299 human cancer cells follows a G1 arrest of both tetraploid and diploid cells due to a failure to initiate DNA synthesis.
      Cabon, L; Sriskandarajah, N; Mui, MZ; Teodoro, JG; Blanchette, P; Branton, PE
      Journal of virology  87  13168-78  2013

      Show Abstract
      Western Blotting24067978 24067978
      Dynamic changes in the genomic localization of DNA replication-related element binding factor during the cell cycle.
      Gurudatta, BV; Yang, J; Van Bortle, K; Donlin-Asp, PG; Corces, VG
      Cell cycle (Georgetown, Tex.)  12  1605-15  2013

      Show Abstract
      Immunofluorescence23624840 23624840

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      Life Science Research > Antibodies and Assays > Primary Antibodies