06-570 Anti-phospho-Histone H3 (Ser10) Antibody, Mitosis Marker

06-570
200 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, H ICC, IP, WB Rb Purified Polyclonal Antibody
      Description
      Catalogue Number06-570
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-phospho-Histone H3 (Ser10) Antibody, Mitosis Marker
      Alternate Names
      • H3 histone, family 3A
      • H3S10P
      • Histone H3 (phospho S10)
      • H3 histone, family 3A
      • H3 histone, family 3B
      • H3 histone, family 3B (H3.3B)
      Background InformationHistone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.
      References
      Product Information
      FormatPurified
      Control
      • Colcemid treated HeLa acid extract
      PresentationPurified rabbit polyclonal serum in buffer containing 0.1 M Tris-glycine, pH 7.4, 0.150 mM NaCl and 0.05% sodium azide.
      Applications
      ApplicationAnti-phospho-Histone H3 (Ser10) Antibody, Mitosis Marker is a Rabbit Polyclonal Antibody for detection of Histone H3 phosphorylated at serine 10.This highly published Ab, also known as Anti-H3S10p, has been validated in ICC, IP & WB.
      Key Applications
      • Immunocytochemistry
      • Immunoprecipitation
      • Western Blotting
      Application NotesImmunocytochemistry Analysis: 1:500 dilution from a representative lot detected Histone H3 in HeLa and A431 cells.
      Immunoprecipitation Analysis: 10 µg from a representative lot immunoprecipitated phospho Histone H3 (Ser10) from HeLa acid extract and Colcemid treated HeLa acid extracted RIPA lysate.
      Beadlyte® Assay Analysis: A representative lot recognizes Histone H3 phosphorylated on Ser10 by Luminex assay.
      Biological Information
      ImmunogenLinear peptide corresponding to human Histone H3 at Ser10.
      EpitopeSer10
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityThis antibody recognizes Histone H3 when phosphorylated at Ser10.
      Species Reactivity
      • Mouse
      • Human
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryHistones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene contains introns and its mRNA is polyadenylated, unlike most histone genes. The protein encoded is a replication-independent member of the histone H3 family.
      Gene Symbol
      • HIST1H3A
      • H3FA
      • HIST1H3B
      • H3FL
      • HIST1H3C
      • H3FC
      • HIST1H3D
      • H3FB
      • HIST1H3E
      • H3FD
      • HIST1H3F
      • H3FI
      • HIST1H3G
      • H3FH
      • HIST1H3H
      • H3FK
      • HIST1H3I
      • H3FF
      • HIST1H3J
      • H3FJ
      • HIST3H3
      • H3FT
      • H3T
      • MGC126886
      Modifications
      • Phosphorylation
      Purification MethodProtein A Purfied
      UniProt Number
      UniProt SummaryFUNCTION: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
      SUBUNIT STRUCTURE: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.
      SUBCELLULAR LOCATION: Nucleus.
      TISSUE SPECIFICITY: Expressed in testicular cells.
      DEVELOPMENTAL STAGE: Expressed during S phase, then expression strongly decreases as cell division slows down during the process of differentiation.
      PTM: Acetylation is generally linked to gene activation. Acetylation on Lys-10 impairs methylation at Arg-9. Acetylation on Lys-19 and Lys-24 favors methylation at Arg-18 By similarity.
      Citrullination at Arg-9 and/or Arg-18 by PADI4 impairs methylation and represses transcription By similarity.
      Asymmetric dimethylation at Arg-18 by CARM1 is linked to gene activation. Symmetric dimethylation at Arg-9 by PRMT5 is linked to gene repression By similarity.
      Methylation at Lys-5, Lys-37 and Lys-80 are linked to gene activation. Methylation at Lys-5 facilitates subsequent acetylation of H3 and H4. Methylation at Lys-80 is associated with DNA double-strand break (DSB) responses and is a specific target for TP53BP1. Methylation at Lys-10 and Lys-28 are linked to gene repression. Methylation at Lys-10 is a specific target for HP1 proteins (CBX1, CBX3 and CBX5) and prevents subsequent phosphorylation at Ser-11 and acetylation of H3 and H4. Methylation at Lys-5 and Lys-80 require preliminary monoubiquitination of H2B at 'Lys-120'. Methylation at Lys-10 and Lys-28 are enriched in inactive X chromosome chromatin By similarity.
      Phosphorylated at Thr-4 by GSG2/haspin during prophase and dephosphorylated during anaphase. At centromeres, specifically phosphorylated at Thr-12 from prophase to early anaphase. Phosphorylated at Ser-11 during the whole mitosis. Phosphorylation at Ser-11, which is linked to gene activation, prevents methylation at Lys-10 but facilitates acetylation of H3 and H4. Phosphorylated at Ser-29 by MLTK isoform 1, RPS6KA5 or AURKB during mitosis or upon ultraviolet B irradiation By similarity.
      Phosphorylation at 'Ser-11' is crucial for chromosome condensation and cell-cycle progression during mitosis and meiosis. In addition phosphorylation at 'Ser-11' is important during interphase because it enables the transcription of genes following external stimulation, like stress or growth factors. Phosphorylation at 'Ser-11' is also an essential regulatory mechanism for neoplastic cell transformation. Phosphorylation at 'Ser-11' by AURKB/Aurora-B mediates the dissociation of HP1 proteins (CBX1, CBX3 and CBX5) from heterochromatin.
      Ubiquitinated By similarity.
      SEQUENCE SIMILIARITIES: Belongs to the histone H3 family.
      Molecular Weight17 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in Colcemid treated HeLa acid extract.
      Western Blot Analysis: 0.5 – 1 µg/ml of this antibody detected Histone H3 on 10 µg of Colcemid treated HeLa acid extract.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions2-8°C for up to 6 months.
      Packaging Information
      Material Size200 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-phospho-Histone H3 (Ser10) Antibody, Mitosis Marker SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-phospho-Histone H3 (Ser10) Antibody, Mitosis Marker Certificates of Analysis

      TitleLot Number
      Anti-phospho Histone H3 (Ser10), -27948672794867
      Anti-phospho Histone H3 (Ser10), -28196512819651
      Anti-phospho Histone H3 (Ser10), Mitosis Marker3076467
      Anti-phospho Histone H3 (Ser10), Mitosis Marker2465253
      Anti-phospho Histone H3 (Ser10), Mitosis Marker - 23701272370127
      Anti-phospho Histone H3 (Ser10), Mitosis Marker - 24303982430398
      Anti-phospho Histone H3 (Ser10), Mitosis Marker - 19572811957281
      Anti-phospho Histone H3 (Ser10), Mitosis Marker - 19924501992450
      Anti-phospho Histone H3 (Ser10), Mitosis Marker - 20660522066052
      Anti-phospho Histone H3 (Ser10), Mitosis Marker - 21899462189946

      References

      Reference overviewApplicationSpeciesPub Med ID
      Cell cycle heterogeneity directs the timing of neural stem cell activation from quiescence.
      Otsuki L, Brand AH
      Science, 360(6384):99-102  2018

      Show Abstract
      29622651 29622651
      Nek2 activation of Kif24 ensures cilium disassembly during the cell cycle.
      Kim, S; Lee, K; Choi, JH; Ringstad, N; Dynlacht, BD
      Nature communications  6  8087  2015

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      26290419 26290419
      Subset of early radial glial progenitors that contribute to the development of callosal neurons is absent from avian brain.
      García-Moreno, F; Molnár, Z
      Proceedings of the National Academy of Sciences of the United States of America  112  E5058-67  2015

      Show Abstract
      26305942 26305942
      The Toll-Like Receptor 5 Agonist Entolimod Mitigates Lethal Acute Radiation Syndrome in Non-Human Primates.
      Krivokrysenko, VI; Toshkov, IA; Gleiberman, AS; Krasnov, P; Shyshynova, I; Bespalov, I; Maitra, RK; Narizhneva, NV; Singh, VK; Whitnall, MH; Purmal, AA; Shakhov, AN; Gudkov, AV; Feinstein, E
      PloS one  10  e0135388  2015

      Show Abstract
      26367124 26367124
      In vitro alterations do not reflect a requirement for host cell cycle progression during Plasmodium liver stage infection.
      Hanson, KK; March, S; Ng, S; Bhatia, SN; Mota, MM
      Eukaryotic cell  14  96-103  2015

      Show Abstract
      25416236 25416236
      The lethal response to Cdk1 inhibition depends on sister chromatid alignment errors generated by KIF4 and isoform 1 of PRC1.
      Voets, E; Marsman, J; Demmers, J; Beijersbergen, R; Wolthuis, R
      Scientific reports  5  14798  2015

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      26423135 26423135
      Pharmacological activation of CB2 receptors counteracts the deleterious effect of ethanol on cell proliferation in the main neurogenic zones of the adult rat brain.
      Rivera, P; Blanco, E; Bindila, L; Alen, F; Vargas, A; Rubio, L; Pavón, FJ; Serrano, A; Lutz, B; Rodríguez de Fonseca, F; Suárez, J
      Frontiers in cellular neuroscience  9  379  2015

      Show Abstract
      26483633 26483633
      Behavior of aberrant chromosome configurations in Drosophila melanogaster female meiosis I.
      Gilliland, WD; Colwell, EM; Lane, FM; Snouffer, AA
      G3 (Bethesda, Md.)  5  175-82  2015

      Show Abstract
      25491942 25491942
      A region-specific neurogenesis mode requires migratory progenitors in the Drosophila visual system.
      Apitz, H; Salecker, I
      Nature neuroscience  18  46-55  2015

      Show Abstract
      25501037 25501037
      The NuRD nucleosome remodelling complex and NHK-1 kinase are required for chromosome condensation in oocytes.
      Nikalayevich, E; Ohkura, H
      Journal of cell science  128  566-75  2015

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      25501812 25501812

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      Life Science Research > Antibodies and Assays > Primary Antibodies