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07-145 | Anti-phospho-Histone H3 (Ser28) Antibody

100 µL  
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      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M WB, ICC, DB, PIA, ChIP Rb Affinity Purified Polyclonal Antibody
      Catalogue Number07-145
      DescriptionAnti-phospho-Histone H3 (Ser28) Antibody
      Alternate Names
      • Histone H3.1t
      • H3/t
      • H3t
      • H3/g
      Background InformationHistone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.
      Product Information
      FormatAffinity Purified
      • HeLa acid extract
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      ApplicationUse Anti-phospho-Histone H3 (Ser28) Antibody (Rabbit Polyclonal Antibody) validated in WB, ICC, DB, PIA, ChIP to detect phospho-Histone H3 (Ser28) also known as H3F3, H3F3B, H3S28P.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      • Dot Blot
      • Peptide Inhibition Assay
      • Chromatin Immunoprecipitation (ChIP)
      Application NotesWestern Blot Analysis: A 1:2,000 dilution from a representative lot detected phospho-Histone H3 (Ser28) in 10 µg of untreated and colcemid treated HeLa acid extract.

      Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected phospho-Histone H3 (Ser28) in HeLa cells.

      Chromatin Immunoprecipitation Analysis: A representative lot immunoprecipitated phospho-Histone H3 (Ser28) in colcecmid treated HeLa cell lysate.

      Dot Blot Specificity Analysis: A representative lot of this antibody detected peptides corresponding to phospho-Histone H3 (Ser28), but not peptides corresponding to other unmodified and modifed Histones.

      Peptide Inhibition Analysis: A 1:500 dilution form a representative was blocked by phospho-Histone H3 (Ser28) peptides, but not non-specific peptides.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to Histone H3 phosphorylated at Ser28.
      EpitopeHistone H3 phosphorylated at Ser28
      SpecificityThis antibody recognizes Histone H3 phosphorylated at Ser28.
      Species Reactivity
      • Human
      • Mouse
      Species Reactivity NoteDemonstrated to react with Human and Mouse. This antibody is expected to demonstrate broad species reactivity due to sequence conservation.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryHistones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
      Gene Symbol
      • HIST3H3
      • H3FT
      • Phosphorylation
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFUNCTION: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

      SUBUNIT STRUCTURE: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.

      SUBCELLULAR LOCATION: Nucleus. Chromosome.
      Molecular Weight~17 kDa observed
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in HeLa acid extract.

      Western Blot Analysis: A 1:2,000 dilution of this antibody detected phospho-Histone H3 (Ser28) in 10 µg of HeLa acid extract.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-phospho-Histone H3 (Ser28) - 2398895 2398895
      Anti-phospho-Histone H3 (Ser28) - 1900508 1900508
      Anti-phospho-Histone H3 (Ser28) - 20175 20175
      Anti-phospho-Histone H3 (Ser28) - 2075955 2075955
      Anti-phospho-Histone H3 (Ser28) - 2210404 2210404
      Anti-phospho-Histone H3 (Ser28) - 23685 23685
      Anti-phospho-Histone H3 (Ser28) - 27707 27707
      Anti-phospho-Histone H3 (Ser28) - DAM1576931 DAM1576931
      Anti-phospho-Histone H3 (Ser28) - DAM1661069 DAM1661069
      Anti-phospho-Histone H3 (Ser28) - DAM1780374 DAM1780374
      Anti-phospho-Histone H3 (Ser28) - DAM1817659 DAM1817659
      Anti-phospho-Histone H3 (Ser28) - DAM1838301 DAM1838301
      Anti-phospho-Histone H3 (Ser28) - JBC1390559 JBC1390559
      Anti-phospho-Histone H3 (Ser28) - JBC1863329 JBC1863329
      Anti-phospho-Histone H3 (Ser28) -2495654 2495654
      Anti-phospho-Histone H3 (Ser28) -2618656 2618656
      Anti-phospho-Histone H3 (Ser28) -2673042 2673042
      Anti-phospho-Histone H3 (Ser28) -2674828 2674828
      Anti-phospho-Histone H3 (Ser28) -2694474 2694474
      Anti-phospho-Histone H3 (Ser28) -2733791 2733791

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