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04-817 | Anti-phospho-Histone H3 (Ser10) Antibody, clone MC463, rabbit monoclonal

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      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H IF, WB, ICC, ChIP-seq, DB, Mplex, ChIP Rb Culture Supernatant Monoclonal Antibody
      Catalogue Number04-817
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-phospho-Histone H3 (Ser10) Antibody, clone MC463, rabbit monoclonal
      Alternate Names
      • H3 histone, family 3A
      • H3S10P
      • Histone H3 (phospho S10)
      • H3 histone, family 3A
      • H3 histone, family 3B
      • H3 histone, family 3B (H3.3B)
      Background InformationHistone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine
      Product Information
      FormatCulture Supernatant
      • UV-treated 293 cell extracts, UV-treated HeLa cell extracts, breast cancer tissue, HEPG2 cell extracts
      PresentationCultured supernantant in 0.05% sodium azide.
      ApplicationAnti-phospho-Histone H3 (Ser10) Antibody, clone MC463 is a rabbit monoclonal antibody for detection of Histone H3 phosphorylated at serine 10. This mAb, also known as H3S10p, is specificity verified using DB & Mplex, published in peer reviewed journals & validated in ChIP, IF, WB, ICC, ChIP-seq.
      Key Applications
      • Immunofluorescence
      • Western Blotting
      • Immunocytochemistry
      • ChIP-seq
      • Dot Blot
      • Multiplexing
      • Chromatin Immunoprecipitation (ChIP)
      Application NotesWestern Blot Analysis:
      A 1:2,000-1:4,000 dilution of this lot detected phosphorylated histone H3 in acid extracted proteins from mitotic HeLa cells (Catalog #17-306) treated with colcemid (lane 1) vs. untreated cells (lane 2) (Figure A).

      Beadlyte® Histone-Peptide Specificity Assay:
      A 1:5,000 dilution of a previous lot was incubated with histone H3 peptides containing various modifications conjugated to Luminex® microspheres. (Figure B). Only the peptide containing phospho-serine 10 was detected.

      Representative image from a previous lot.
      Positive chromosome immunostaining for mitotic HeLa cells and A431 cells.

      Chromatin Immunoprecipitation:
      A previous lot was shown to be suitable for ChIP by an independent laboratory.
      Biological Information
      ImmunogenPeptide containing the sequence RK[pS]TG. in which pS corresponds to phospho-serine at position 10 of human histone H3
      SpecificityHistone H3 phosphorylated at Serine 10
      Species Reactivity
      • Human
      Species Reactivity NoteBroad species cross-reactivity is expected
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryHistones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
      Gene Symbol
      • HIST1H3A
      • H3FA
      • HIST1H3B
      • H3FL
      • HIST1H3C
      • H3FC
      • HIST1H3D
      • H3FB
      • HIST1H3E
      • H3FD
      • HIST1H3F
      • H3FI
      • HIST1H3G
      • H3FH
      • HIST1H3H
      • H3FK
      • HIST1H3I
      • H3FF
      • HIST1H3J
      • H3FJ
      • HIST3H3
      • H3FT
      • H3T
      • MGC126886
      • Phosphorylation
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q16695 # Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
      SIZE: 136 amino acids; 15508 Da
      SUBUNIT: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.
      TISSUE SPECIFICITY: Expressed in testicular cells.
      DEVELOPMENTAL STAGE: Expressed during S phase, then expression strongly decreases as cell division slows down during the process of differentiation.
      PTM: Acetylation is generally linked to gene activation. Acetylation on Lys-10 impairs methylation at Arg-9. Acetylation on Lys-19 and Lys-24 favors methylation at Arg-18 (By similarity). & Citrullination at Arg-9 and/or Arg-18 by PADI4 impairs methylation and represses transcription (By similarity). & Asymmetric dimethylation at Arg-18 by CARM1 is linked to gene activation. Symmetric dimethylation at Arg-9 by PRMT5 is linked to gene repression (By similarity). & Methylation at Lys-5, Lys-37 and Lys-80 are linked to gene activation. Methylation at Lys-5 facilitates subsequent acetylation of H3 and H4. Methylation at Lys-80 is associated with DNA double-strand break (DSB) responses and is a specific target for TP53BP1. Methylation at Lys-10 and Lys-28 are linked to gene repression. Methylation at Lys-10 is a specific target for HP1 proteins (CBX1, CBX3 and CBX5) and prevents subsequent phosphorylation at Ser-11 and acetylation of H3 and H4. Methylation at Lys-5 and Lys-80 require preliminary monoubiquitination of H2B at 'Lys-120'. Methylation at Lys-10 and Lys-28 are enriched in inactive X chromosome chromatin (By similarity). & Phosphorylated at Thr-4 by GSG2/haspin during prophase and dephosphorylated during anaphase. At centromeres, specifically phosphorylated at Thr-12 from prophase to early anaphase. Phosphorylated at Ser-11 during the whole mitosis. Phosphorylation at Ser-11, which is linked to gene activation, prevents methylation at Lys-10 but facilitates acetylation of H3 and H4. Phosphorylated at Ser-29 by MLTK isoform 1, RPS6KA5 or AURKB during mitosis or upon ultraviolet B irradiation (By similarity). & Phosphorylation at 'Ser-11' is crucial for chromosome condensation and cell-cycle progression during mitosis and meiosis. In addition phosphorylation at 'Ser-11' is important during interphase because it enables the transcription of genes following external stimulation, like stress or growth factors. Phosphorylation at 'Ser-11' is also an essential regulatory mechanism for neoplastic cell transformation. Phosphorylation at 'Ser-11' by AURKB/Aurora-B mediates the dissociation of HP1 proteins (CBX1, CBX3 and CBX5) from heterochromatin. & Ubiquitinated (By similarity).
      SIMILARITY: SwissProt: Q16695 ## Belongs to the histone H3 family.
      Molecular Weight17kDa
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by immunoblot on acid extracted proteins from mitotic HeLa cells
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-phospho-Histone H3 (Ser10), -2818712 2818712
      Anti-phospho-Histone H3 (Ser10), clone MC463 - 2135139 2135139
      Anti-phospho-Histone H3 (Ser10), clone MC463 - 2387819 2387819
      Anti-phospho-Histone H3 (Ser10), clone MC463 - 2453256 2453256
      Anti-phospho-Histone H3 (Ser10), clone MC463 - 2239644 2239644
      Anti-phospho-Histone H3 (Ser10), clone MC463 - DAM1594221 DAM1594221
      Anti-phospho-Histone H3 (Ser10), clone MC463 - DAM1598813 DAM1598813
      Anti-phospho-Histone H3 (Ser10), clone MC463 - JBC1793800 JBC1793800
      Anti-phospho-Histone H3 (Ser10), clone MC463 - LV1508850 LV1508850
      Anti-phospho-Histone H3 (Ser10), clone MC463 - NG1630104 NG1630104
      Anti-phospho-Histone H3 (Ser10), clone MC463 - NG1667635 NG1667635
      Anti-phospho-Histone H3 (Ser10), clone MC463 - NG1710274 NG1710274
      Anti-phospho-Histone H3 (Ser10), clone MC463 - NG1748979 NG1748979
      Anti-phospho-Histone H3 (Ser10), clone MC463 - NG1839560 NG1839560
      Anti-phospho-Histone H3 (Ser10), clone MC463 - NG1867615 NG1867615
      Anti-phospho-Histone H3 (Ser10), clone MC463 -2510352 2510352
      Anti-phospho-Histone H3 (Ser10), clone MC463 -2664240 2664240
      Anti-phospho-Histone H3 (Ser10), clone MC463 -2700779 2700779
      Anti-phospho-Histone H3 (Ser10), clone MC463 -2739250 2739250

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      Genetics 193 77-84 2013

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      Western Blotting21825075 21825075
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