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05-806-AF488 | Anti-phospho-Histone H3 (Ser10) Antibody, clone 3H10, Alexa Fluor® 488

100 µL  
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      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H ICC M AlexaFluor®488 Monoclonal Antibody
      Catalogue Number05-806-AF488
      DescriptionAnti-phospho-Histone H3 (Ser10) Antibody, clone 3H10, Alexa Fluor® 488
      Alternate Names
      • H3S10P
      • Histone H3, Ser10 phosphorylated
      • H3 histone, family 3A, Ser10 phosphorylated
      • H3 histone, family 3B, Ser10 phosphorylated
      • H3.3B, Ser10 phosphorylated
      Background InformationHistone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine. Histone H3 variants (H3.1, H3.2 and H3.3) have been implicated in the epigenetic memory of cellular state. Genome-wide patterns of H3 are dependent on amino acid sequence and change with cellular differentiation at developmentally regulated loci.
      Product Information
      PresentationPurified mouse monoclonal IgG1κ antibody conjugate in PBS with 15 mg/mL BSA and 0.1 % sodium azide.
      ApplicationThis Anti-phospho-Histone H3 (Ser10) Antibody, clone 3H10, Alexa Fluor® 488 is validated for use in Immunocytochemistry for the detection of phospho-Histone H3.
      Key Applications
      • Immunocytochemistry
      Application NotesImmunocytochemistry Analysis: A 1:100 dilution from a representative lot detected histone H3 Ser10 phosphorylation in A431 cells.
      The unconjugated antibody (Cat. No. 05-806) is shown to be suitable also for flow cytometry, multiplex assay, chromatin Western blotting, immunogen peptide inhibition assay, immunofluorescence and immunocytochemistry applications.
      Biological Information
      ImmunogenA proprietary immunogen based on a peptide sequence containing phospho-serine corresponding to residue 10 of human histone H3.
      Epitope Histone H3 pSer10.
      ConcentrationPlease refer to lot specific datasheet.
      SpecificityHistone H3 phosphorylated at Ser10.
      Species Reactivity
      • Human
      Species Reactivity NoteHuman. Broad species cross-reactivity is expected.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • HIST1H3A
      • H3T
      • H3FT
      • HIST3H3
      • H3FJ
      • HIST1H3J
      • H3FF
      • HIST1H3I
      • H3FK
      • HIST1H3H
      • H3FH
      • HIST1H3G
      • H3FI
      • HIST1H3F
      • H3FD
      • HIST1H3E
      • H3FB
      • HIST1H3D
      • H3FC
      • HIST1H3C
      • H3FL
      • HIST1H3B
      • H3FA
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~17 kDa observed.
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunocytochemistry in HeLa cells.

      Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected histone H3 Ser10 phosphorylation in HeLa cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information