A part of MilliporeSigma

16-193 | Anti-phospho-Histone H2A.X (Ser139) Antibody, clone JBW301, biotin conjugate

16-193
100 µg  
Retrieving price...
Price could not be retrieved
Minimum Quantity needs to be mulitiple of
Upon Order Completion More Information
You Saved ()
 
Request Pricing
Limited AvailabilityLimited Availability
Stocked 
Discontinued
Limited Quantities Available
Available
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service

      Special Offers

       

      Contact Customer Service

      Click To Print This Page

      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H ICC, WB M Biotin Monoclonal Antibody
      Description
      Catalogue Number16-193
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-phospho-Histone H2A.X (Ser139) Antibody, clone JBW301, biotin conjugate
      Alternate Names
      • H2AXS139P
      • Histone H2A.X (phospho S139)
      • H2A histone family, member X
      • H2AX histone
      Background InformationHistones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures.
      References
      Product Information
      FormatBiotin
      Control
      • Staurosporine-treated Jurkat cell lysates.
      PresentationPurified biotin conjugated mouse monoclonal IgG1 in buffer containing 70% storage buffer (0.02 M Phosphate buffer, pH7.6, 0.25 M NaCl, 0.1% sodium azide) and 30% glycerol
      Applications
      ApplicationAnti-phospho-Histone H2A.X (Ser139) Antibody, clone JBW301, biotin conjugate is a Mouse Monoclonal Antibody for detection of phospho-Histone H2A.X (Ser139) also known as H2AXS139P, H2AX histone & has been validated in ICC & WB.
      Key Applications
      • Immunocytochemistry
      • Western Blotting
      Biological Information
      ImmunogenKLH-conjugated, synthetic peptide (CKATQA[pS]QEY) corresponding to amino acids 134-142 of human histone H2A.X. The immunizing sequence has 8 identical amino acids in yeast and mouse.
      EpitopeSer139
      CloneJBW301
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityRecognizes Histone H2A.X phosphorylated at Ser139, Mr 15kDa.
      IsotypeIgG1
      Species Reactivity
      • Human
      Species Reactivity NoteHuman. A broad species cross-reactivity is expected based on conservation of sequence homology.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryHistones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif.
      Gene Symbol
      • H2AFX
      • H2AX
      • H2a/x
      • H2A/X
      • H2A.X
      Modifications
      • Phosphorylation
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P16104 # Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C- terminal phosphorylation.
      SIZE: 143 amino acids; 15145 Da
      SUBUNIT: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. Interacts with numerous proteins required for DNA damage signaling and repair when phosphorylated on Ser-140. These include MDC1, TP53BP1, BRCA1 and the MRN complex, composed of MRE11A, RAD50, and NBN. Interaction with the MRN complex is mediated at least in part by NBN. Also interacts with DHX9/NDHII when phosphorylated on Ser-140.
      SUBCELLULAR LOCATION: Nucleus.
      DEVELOPMENTAL STAGE: Synthesized in G1 as well as in S-phase.
      DOMAIN: SwissProt: P16104 The [ST]-Q motif constitutes a recognition sequence for kinases from the PI3/PI4-kinase family.
      PTM: Phosphorylated on Ser-140 (to form gamma-H2AFX) in response to DNA double strand breaks (DSBs) generated by exogenous genotoxic agents and by stalled replication forks, and may also occur during meiotic recombination events and immunoglobulin class switching in lymphocytes. Phosphorylation can extend up to several thousand nucleosomes from the actual site of the DSB and may mark the surrounding chromatin for recruitment of proteins required for DNA damage signaling and repair. Widespread phosphorylation may also serve to amplify the damage signal or aid repair of persistent lesions. Phosphorylation of Ser-140 in response to ionizing radiation is mediated by both ATM and PRKDC while defects in DNA replication induce Ser-140 phosphorylation subsequent to activation of ATR and PRKDC. Dephosphorylation of Ser-140 by PP2A is required for DNA DSB repair. In meiosis, Ser-140 phosphorylation may occur at synaptonemal complexes during leptotene as an ATM-dependent response to the formation of programmed DSBs by SPO11. Ser-140 phosphorylation may subsequently occurs at unsynapsed regions of both autosomes and the XY bivalent during zygotene, downstream of ATR and BRCA1 activation. Ser-140 phosphorylation may also be required for transcriptional repression of unsynapsed chromatin and meiotic sex chromosome inactivation (MSCI), whereby the X and Y chromosomes condense in pachytene to form the heterochromatic XY-body. During immunoglobulin class switch recombination in lymphocytes, Ser-140 phosphorylation may occur at sites of DNA-recombination subsequent to activation of the activation-induced cytidine deaminase AICDA. & Monoubiquitination of Lys-120 by RING1 and RNF2/RING2 complex gives a specific tag for epigenetic transcriptional repression (By similarity).
      SIMILARITY: Belongs to the histone H2A family.
      Molecular Weight15 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated in acid extracted histone from Jurkat cell lysates treated with 0.5mM staurosporine (19-123).

      Western Blot Analysis:
      0.5-1 μg/mL of this lot detected phosphorylated histone H2A.X (Ser139) in acid extracted histone from Jurkat cell lysates treated with 0.5μM staurosporine (Catalog # 19-123).
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20ºC from date of receipt.
      Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerolcontaining solutions to become frozen during storage.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-phospho-Histone H2A.X (S139), clone JBW301, biotin conjugate (mouse monoclonal IgG1) - DAM1460180 DAM1460180
      Anti-phospho-Histone H2A.X (Ser139), clone JBW301, biotin conjugate - 2147312 2147312
      Anti-phospho-Histone H2A.X (Ser139), clone JBW301, biotin conjugate - 2342868 2342868
      Anti-phospho-Histone H2A.X (Ser139), clone JBW301, biotin conjugate - 2388944 2388944
      Anti-phospho-Histone H2A.X (Ser139), clone JBW301, biotin conjugate - 2028742 2028742
      Anti-phospho-Histone H2A.X (Ser139), clone JBW301, biotin conjugate - 22238 22238
      Anti-phospho-Histone H2A.X (Ser139), clone JBW301, biotin conjugate - 2287717 2287717
      Anti-phospho-Histone H2A.X (Ser139), clone JBW301, biotin conjugate - 32503 32503
      Anti-phospho-Histone H2A.X (Ser139), clone JBW301, biotin conjugate - DAM1460180 DAM1460180
      Anti-phospho-Histone H2A.X (Ser139), clone JBW301, biotin conjugate - DAM1576386 DAM1576386
      Anti-phospho-Histone H2A.X (Ser139), clone JBW301, biotin conjugate - DAM1588179 DAM1588179
      Anti-phospho-Histone H2A.X (Ser139), clone JBW301, biotin conjugate - DAM1753205 DAM1753205
      Anti-phospho-Histone H2A.X (Ser139), clone JBW301, biotin conjugate - DAM1791480 DAM1791480
      Anti-phospho-Histone H2A.X (Ser139), clone JBW301, biotin conjugate - DAM1808154 DAM1808154
      Anti-phospho-Histone H2A.X (Ser139), clone JBW301, biotin conjugate - DAM1831484 DAM1831484
      Anti-phospho-Histone H2A.X (Ser139), clone JBW301, biotin conjugate - JBC1871065 JBC1871065
      Anti-phospho-Histone H2A.X (Ser139), clone JBW301, biotin conjugate - JBC1924341 JBC1924341
      Anti-phospho-Histone H2A.X (Ser139), clone JBW301, biotin conjugate -2500593 2500593
      Anti-phospho-Histone H2A.X (Ser139), clone JBW301, biotin conjugate -2575736 2575736
      Anti-phospho-Histone H2A.X (Ser139), clone JBW301, biotin conjugate -2593142 2593142
      Anti-phospho-Histone H2A.X (Ser139), clone JBW301, biotin conjugate -2593150 2593150
      Anti-phospho-Histone H2A.X (Ser139), clone JBW301, biotin conjugate -2763994 2763994

      References

      Reference overviewApplicationPub Med ID
      Quantitative model of cell cycle arrest and cellular senescence in primary human fibroblasts.
      Schäuble, S; Klement, K; Marthandan, S; Münch, S; Heiland, I; Schuster, S; Hemmerich, P; Diekmann, S
      PloS one 7 e42150 2012

      Show Abstract
      22879912 22879912
      Down-regulation of HtrA1 activates the epithelial-mesenchymal transition and ATM DNA damage response pathways.
      Wang, N; Eckert, KA; Zomorrodi, AR; Xin, P; Pan, W; Shearer, DA; Weisz, J; Maranus, CD; Clawson, GA
      PloS one 7 e39446 2012

      Show Abstract
      22761798 22761798
      γ-H2AX detection in peripheral blood lymphocytes, splenocytes, bone marrow, xenografts, and skin.
      Christophe E Redon,Asako J Nakamura,Olivier Sordet,Jennifer S Dickey,Ksenia Gouliaeva,Brian Tabb,Scott Lawrence,Robert J Kinders,William M Bonner,Olga A Sedelnikova
      Methods in molecular biology (Clifton, N.J.) 682 2011

      Show Abstract
      21057933 21057933
      RSPO1/β-catenin signaling pathway regulates oogonia differentiation and entry into meiosis in the mouse fetal ovary.
      Chassot, AA; Gregoire, EP; Lavery, R; Taketo, MM; de Rooij, DG; Adams, IR; Chaboissier, MC
      PloS one 6 e25641 2011

      Show Abstract
      21991325 21991325
      Characterization of oxidative guanine damage and repair in mammalian telomeres.
      Wang, Z; Rhee, DB; Lu, J; Bohr, CT; Zhou, F; Vallabhaneni, H; de Souza-Pinto, NC; Liu, Y
      PLoS genetics 6 e1000951 2010

      Show Abstract
      Immunofluorescence20485567 20485567
      Phosphorylation of H2AX at short telomeres in T cells and fibroblasts.
      Hao, LY; Strong, MA; Greider, CW
      The Journal of biological chemistry 279 45148-54 2004

      Show Abstract
      Immunocytochemistry15322096 15322096
      Characterisation of cytotoxicity and DNA damage induced by the topoisomerase II-directed bisdioxopiperazine anti-cancer agent ICRF-187 (dexrazoxane) in yeast and mammalian cells.
      Jensen, LH; Dejligbjerg, M; Hansen, LT; Grauslund, M; Jensen, PB; Sehested, M
      BMC pharmacology 4 31 2004

      Show Abstract
      Western Blotting15575955 15575955

      Related Products & Applications

      Related Products

      Catalog Number Description  
      05-636 Anti-phospho-Histone H2A.X (Ser139) Antibody, clone JBW301 Show Pricing & Availability
      05-636-AF488 Anti-phospho Histone H2A.X (Ser139), clone JBW301, Alexa Fluor® 488 Conjugate Antibody Show Pricing & Availability
      05-636-AF555 Anti-phospho-H2A.X Antibody (Ser139), clone JBW30, Alexa Fluor® 555 Conjugate Show Pricing & Availability
      05-636-AF647 Anti-phospho Histone H2A.X (Ser139) Antibody, clone JBW301, Alexa Fluor® 647 Show Pricing & Availability
      05-636-I Anti-phospho-Histone H2A.X (Ser139) Antibody, clone JBW301 Show Pricing & Availability
      07-1590 Anti-phospho Histone H2A.X Antibody (Tyr142) Show Pricing & Availability
      07-164 Anti-phospho-H2A.X (Ser139) Antibody Show Pricing & Availability
      07-164-AF647 Anti-phospho H2A.X (Ser139) Antibody, Alexa Fluor® 647 Conjugate Show Pricing & Availability
      07-289 Anti-acetyl-Histone H2A (Lys9) Antibody Show Pricing & Availability
      07-290 Anti-acetyl-Histone H2A (Lys5) Antibody Show Pricing & Availability
      07-376 Anti-acetyl-Histone H2A Antibody Show Pricing & Availability
      07-627 Anti-Histone H2A.X Antibody Show Pricing & Availability
      16-202A Anti-phospho-Histone H2A.X (Ser139) Antibody, clone JBW301, FITC conjugate Show Pricing & Availability
      AB10020 Anti-acetyl-Histone H2A.X (Lys5) Antibody Show Pricing & Availability
      AB10021 Anti-Histone H2A.X Antibody,Chicken Show Pricing & Availability
      AB10022 Anti-Histone H2A.X Antibody Show Pricing & Availability
      AB10029 Anti-Ubiquityl Histone H2A.X (Lys119) Antibody Show Pricing & Availability
      ABE1457 Anti-phospho-H2A.X (Tyr142) Antibody Show Pricing & Availability
      ABE1960 Anti-Histone H2A.X Antibody Show Pricing & Availability
      MABE171 Anti-phospho-Histone H2A.X (Thr120) Antibody, clone 11F5.3 Show Pricing & Availability
      MABE205 Anti-phospho-Histone H2A.X (Ser139) Antibody, clone EP854(2)Y, rabbit monoclonal Show Pricing & Availability

      Alternative Format

      Catalog Number Description  
      05-636 Anti-phospho-Histone H2A.X (Ser139) Antibody, clone JBW301 Show Pricing & Availability
      16-202A Anti-phospho-Histone H2A.X (Ser139) Antibody, clone JBW301, FITC conjugate Show Pricing & Availability

      Product Families

      View Gene Related Products for this Target

      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies