A part of MilliporeSigma

ABE418 | Anti-phospho Histone H2A Antibody (Thr120)

100 µg  
Retrieving price...
Price could not be retrieved
Minimum Quantity needs to be mulitiple of
Upon Order Completion More Information
You Saved ()
Request Pricing
Limited AvailabilityLimited Availability
Limited Quantities Available
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service

      Special Offers


      Contact Customer Service

      Click To Print This Page


      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, R WB, DB, IP Rb Affinity Purified Polyclonal Antibody
      Catalogue NumberABE418
      DescriptionAnti-phospho Histone H2A Antibody (Thr120)
      Alternate Names
      • Histone H2A type 1
      • H2A.1
      • Histone H2A/p
      Background InformationHistone H2A is one of four components of the core nucleosomal structure. The nucleosome represents a unit of chromatin in which DNA is wrapped around a histone octamer. Histones undergo a number of post-translational modifications (PTM) in response to various stimuli that may induce changes in the structure of the nucleosome and hide or expose DNA sequences. Histone H2A, in particular, undergoes acetylation on lysine 5 by the Tip60 enzyme which may promote unfolding of chromatin and transcription. In addition, H2A is phosphorylated on serine 1 and serine 139 (H2A.X) residues which mark mitosis and gene repression, and DNA damage, respectively. Gene repression may also result from the sumoylation of H2A’s lysine 126 residue. In addition, ubiquitination of the H2A lysine 119 residue may play a role in spermatogenesis. It is the pattern of different modifications on several histone residues that alter chromatin, rather than modification of single residues.
      Product Information
      FormatAffinity Purified
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      ApplicationDetect Histone H2A using this rabbit polyclonal antibody, Anti-phospho Histone H2A Antibody (Thr120) validated for use in western blotting, Dot Blot & IP.
      Key Applications
      • Western Blotting
      • Dot Blot
      • Immunoprecipitation
      Application NotesImmunoprecipitation Analysis: 10 µg from a representative lot immunoprecipitated phospho Histone H2A (Thr120) in 500 µg of colcemid treated HeLa acid extract.

      Dot Blot (Specificity) Analysis: 0.5 µg/mL from a representative lot detected phospho Histone H2A (Thr120) and not non-phosphorylated Histone H2A peptide or other peptides corresponding to modified histones.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to the C-terminus of human Histone H2A phosphorylated at Thr120.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • HIST1H2AG
      • H2AFP
      • HIST1H2AI
      • H2AFC
      • HIST1H2AK
      • H2AFD
      • HIST1H2AL
      • H2AFI
      • HIST1H2AM
      • H2AFN
      • Phosphorylation
      Purification MethodAffinity Purfied
      UniProt Number
      Molecular Weight~16 kDa observed
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in untreated and colcemid treated HeLa acid extract.

      Western Blotting Analysis: 0.25 µg/mL of this antibody detected phospho Histone H2A (Thr120) in 10 µg of colcemid treated HeLa acid extract, and demonstrated a decrease in signal in untreated HeLa acid extract.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information

      Related Products & Applications

      Related Products

      Catalog Number Description  
      ABE327 Anti-Histone H2A Antibody Show Pricing & Availability
      ABE569 Anti-monoubiquitin H2A Antibody (Lys119) Show Pricing & Availability

      View Gene Related Products for this Target


      Life Science Research > Antibodies and Assays > Primary Antibodies