05-1324 Anti-phospho-Histone H1 Antibody, clone 12D11

05-1324
200 µL  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, B, M, Vrt IHC, ICC, IP, WB M Culture Supernatant Monoclonal Antibody
      Description
      Catalogue Number05-1324
      DescriptionAnti-phospho-Histone H1 Antibody, clone 12D11
      Alternate Names
      • H1 histone family, member 5
      • Histone H1a
      • histone 1
      • H1b
      • histone cluster 1
      • H1b
      Background InformationHistones are highly conserved proteins that serve as the structural scaffold for the organization of nuclear DNA into chromatin. The four core histones, H2A, H2B, H3, and H4, assemble into an octamer (2 molecules of each). Subsequently, 146 base pairs of DNA are wrapped around the octamer, forming a nucleosome. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into 30nm chromatin fibers and higher order structures.
      References
      Product Information
      FormatCulture Supernatant
      Control
      • HeLa cell lysate, treated with colcemid.
      PresentationSupplied as 200 μL of culture supernatant with 0.05% sodium azide.
      Applications
      ApplicationUse Anti-phospho-Histone H1 Antibody, clone 12D11 (Mouse Monoclonal Antibody) validated in IHC, ICC, IP, WB to detect phospho-Histone H1 also known as Histone H1a, histone 1 H1b.
      Key Applications
      • Immunohistochemistry
      • Immunocytochemistry
      • Immunoprecipitation
      • Western Blotting
      Application NotesImmunohistochemistry: This antibody has been reported by an independent laboratory to detect CDK (cyclin dependent kinase) phosphorylated Histone H1 in benign tissue sections.1

      Immunocytochemistry:
      This antibody has been demonstrated by an outside laboratory to show positive immunostaining for phosphorylated histone H1 in HUSK (human fetal primary myoblasts) cells fixed with 3.7% paraformaldehyde followed by permeabilization with 0.2% Triton X-100.2

      Immunoprecipitation:
      This antibody has been demonstrated by an outside laboratory to immunoprecipitate phosphorylated histone H1 using solid phase beads bound to anti-mouse IgM.
      Biological Information
      ImmunogenThe immunogen was KLH-conjugated bovine nuclear extract.
      Clone12D11
      HostMouse
      SpecificityRecognizes phosphorylated Histone H1, Mr 32 kDa.
      IsotypeIgMκ
      Species Reactivity
      • Human
      • Bovine
      • Mouse
      • Vertebrates
      Species Reactivity NoteHuman, mouse, bovine. Broad species cross-reactivity is expected, based on sequence homology.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryHistones are basic nuclear proteins responsible for nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is
      wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H1 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6. [provided by RefSeq].
      Gene Symbol
      • H1
      • H1.5
      • H1F5
      • MGC126630
      • MGC126632
      • OTTHUMP00000017748
      Modifications
      • Phosphorylation
      UniProt Number
      UniProt SummaryFUNCTION: Histones H1 are necessary for the condensation of nucleosome chains into higher order structures.

      SUBCELLULAR LOCATION: Nucleus.

      SIMILARITY: Belongs to the histone H1/H5 family.

      Molecular Weight~32 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceWestern Blot Analysis:
      1:1000 dilution of this antibody detected Histone H1 in colcemid-treated HeLa lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt. Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size200 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-phospho-Histone H1 Antibody, clone 12D11 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-phospho-Histone H1 Antibody, clone 12D11 Certificates of Analysis

      TitleLot Number
      Anti-phospho-Histone H1, clone 12D113072319
      Anti-phospho-Histone H1, clone 12D11 - 24204432420443
      Anti-phospho-Histone H1, -25670172567017
      Anti-phospho-Histone H1, -26944602694460
      Anti-phospho-Histone H1, -27163072716307
      Anti-phospho-Histone H1, -27392592739259
      Anti-phospho-Histone H1, -27502672750267
      Anti-phospho-Histone H1, -27804542780454
      Anti-phospho-Histone H1, clone 12D11 - 19759451975945
      Anti-phospho-Histone H1, clone 12D11 - 20327422032742

      References

      Reference overviewApplicationPub Med ID
      Lasonolide A, a potent and reversible inducer of chromosome condensation.
      Zhang, YW; Ghosh, AK; Pommier, Y
      Cell cycle (Georgetown, Tex.)  11  4424-35  2012

      Show Abstract
      Western Blotting23159859 23159859
      Growth factors that repress myoblast differentiation sustain phosphorylation of a specific site on histone H1.
      Cole, F, et al.
      J. Biol. Chem., 268: 1580-5 (1993)  1993

      Show Abstract
      7678408 7678408

      Technical Info

      Title
      White Paper - The Message in the Marks: Deciphering Cancer Epigenetics (EMD)