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06-597 | Anti-phospho-Histone H1 Antibody

200 µg  
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      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      B, H ICC, WB Rb Purified Polyclonal Antibody
      Catalogue Number06-597
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-phospho-Histone H1 Antibody
      Background InformationHistone H1 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H1 is involved with the structure of the nucleosomes of the 'beads on a string' structure.

      The N-terminal tail of histone H1 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.
      Product Information
      • Colcemid treated HeLa cell lysate
      PresentationImmunoaffinity Purified immunoglobulin in 0.02M Phosphate Buffer, 0.25 M NaCl, pH 7.6 with 15 mg/mL BSA containing no preservatives.
      ApplicationAnti-phospho-Histone H1 Antibody is a high quality Rabbit Polyclonal Antibody for the detection of phospho-Histone H1 & has been validated in ICC & WB.
      Key Applications
      • Immunocytochemistry
      • Western Blotting
      Biological Information
      ImmunogenHyper-phosphorylated isoform of tetrahymena Histone H1 purified by cation-exchange HPLC
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      SpecificityRecognizes phospho-Histone H1, Mr 32 kDa. Additional proteins were also detected, Mr 66 and 100 kDa.
      Species Reactivity
      • Bovine
      • Human
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryHistones are basic nuclear proteins responsible for nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H1 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6.
      Gene Symbol
      • HIST1H1C
      • MGC3992
      • H1F2
      • H1.2
      • Phosphorylation
      Purification MethodProtein A Purfied
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P16403 # Histones H1 are necessary for the condensation of nucleosome chains into higher order structures.
      SIZE: 213 amino acids; 21365 Da
      SIMILARITY: SwissProt: P16403 ## Belongs to the histone H1/H5 family.
      Molecular Weight32 kDa
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceImmunoblot Analysis: 0.5-2 μg/mL detected phosphorylated Histone H1 from acid extracts of colcemid treated HeLa cells but not untreated HeLa cells (Catalog # 17-306).

      Immunocytochemistry: 5-10 μg/mL showed positive immunostaining for Histone H1 in mitotic HeLa cells fixed with 95% ethanol/5% glacial acetic acid (v/v).
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
      Packaging Information
      Material Size200 µg
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-phospho-Histone H1 2474895
      Anti-phospho-Histone H1 - 15794 15794
      Anti-phospho-Histone H1 - 22163 22163
      Anti-phospho-Histone H1 - 24722 24722
      Anti-phospho-Histone H1 - 32078 32078

      References | 14 Available | See All References

      Reference overviewApplicationPub Med ID
      Global chromatin fibre compaction in response to DNA damage.
      Hamilton, C; Hayward, RL; Gilbert, N
      Biochemical and biophysical research communications 414 820-5 2011

      Show Abstract
      Western Blotting22020103 22020103
      Silencing of CDK5 reduces neurofibrillary tangles in transgenic alzheimer's mice.
      Piedrahita, D; Hernández, I; López-Tobón, A; Fedorov, D; Obara, B; Manjunath, BS; Boudreau, RL; Davidson, B; Laferla, F; Gallego-Gómez, JC; Kosik, KS; Cardona-Gómez, GP
      The Journal of neuroscience : the official journal of the Society for Neuroscience 30 13966-76 2010

      Show Abstract
      Western Blotting20962218 20962218
      Cyclin-dependent kinase 1-mediated Bcl-xL/Bcl-2 phosphorylation acts as a functional link coupling mitotic arrest and apoptosis.
      David T Terrano,Meenakshi Upreti,Timothy C Chambers
      Molecular and cellular biology 30 2010

      Show Abstract Full Text Article
      19917720 19917720
      Activation of cyclin-dependent kinase 5 is a consequence of cell death.
      Yixia Ye,Antonella Tinari,Walter Malorni,Richard A Lockshin,Zahra Zakeri
      Journal of biomedicine & biotechnology 2009 2009

      Show Abstract Full Text Article
      19830249 19830249
      Cdk5 regulates STAT3 activation and cell proliferation in medullary thyroid carcinoma cells.
      Lin, H; Chen, MC; Chiu, CY; Song, YM; Lin, SY
      The Journal of biological chemistry 282 2776-84 2007

      Show Abstract
      17145757 17145757
      Characterization of vinblastine-induced Bcl-xL and Bcl-2 phosphorylation: evidence for a novel protein kinase and a coordinated phosphorylation/dephosphorylation cycle associated with apoptosis induction.
      Du, L; Lyle, CS; Chambers, TC
      Oncogene 24 107-17 2005

      Show Abstract
      15531923 15531923
      Sulforaphane: a naturally occurring mammary carcinoma mitotic inhibitor, which disrupts tubulin polymerization.
      Jackson, SJ; Singletary, KW
      Carcinogenesis 25 219-27 2004

      Show Abstract
      Western Blotting14578157 14578157
      RLIP, an effector of the Ral GTPases, is a platform for Cdk1 to phosphorylate epsin during the switch off of endocytosis in mitosis
      Rosse, C., et al
      J Biol Chem, 278:30597-604 (2003) 2003

      Immunoblotting (Western)12775724 12775724
      Rapid activation of G2/M checkpoint after hypertonic stress in renal inner medullary epithelial (IME) cells is protective and requires p38 kinase.
      Dmitrieva, NI; Bulavin, DV; Fornace, AJ; Burg, MB
      Proceedings of the National Academy of Sciences of the United States of America 99 184-9 2002

      Show Abstract
      11756692 11756692
      A novel regulatory element determines the timing of Mos mRNA translation during Xenopus oocyte maturation.
      Amanda Charlesworth, John A Ridge, Leslie A King, Melanie C MacNicol, Angus M MacNicol
      The EMBO journal 21 2798-806 2002

      Show Abstract Full Text Article
      12032092 12032092

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      Life Science Research > Antibodies and Assays > Primary Antibodies