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04-1144 | Anti-phospho-Her4/ErbB-4 (Tyr1258) Antibody, clone EP2272AY, rabbit monoclonal

04-1144
100 µL  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H WB Rb Unpurified Monoclonal Antibody
      Description
      Catalogue Number04-1144
      DescriptionAnti-phospho-Her4/ErbB-4 (Tyr1258) Antibody, clone EP2272AY, rabbit monoclonal
      Alternate Names
      • Tyrosine kinase-type cell surface receptor HER4
      • avian erythroblastic leukemia viral (v-erb-b2) oncogene
        homolog 4
      • receptor tyrosine-protein kinase erbB-4
      • v-erb-a avian erythroblastic leukemia viral oncogene
        homolog-like 4
      • v-erb-a erythroblastic leukemia viral oncogene homolog 4
      • v-erb-a erythroblastic leukemia viral oncogene homolog 4 (avian)
      Background InformationErbB4/Her4 is a member of the ErbB receptor family of tyrosine kinases. It is largely expressed in many breast carcinoma cell lines; however, it is also found in normal skeletal muscle, heart, pituitary, brain, and cerebellum. This receptor is more specified than the others in this family because of its unique ability of the C-terminal to bind to a PDZ domain.
      References
      Product Information
      FormatUnpurified
      Control
      • SKBR3 cell lysate
      PresentationRabbit Monoclonal in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl containing 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
      Applications
      ApplicationThis Anti-phospho-Her4/ErbB-4 (Tyr1258) Antibody, clone EP2272AY, Rabbit is validated for use in WB for the detection of phospho-Her4/ErbB-4 (Tyr1258).
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenPhospho-specific peptide corresponding to residues surrounding Tyr1258 of human Her4/ErbB-4.
      EpitopePhosphorylated Tyr1258
      CloneEP2272AY
      HostRabbit
      SpecificityThis antibody detects Her4/ErbB-4 phosphorylated on Tyr258.
      IsotypeIgG
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • EC 2.7.10.1
      • HER4
      • MGC138404
      • p180erbB4
      Modifications
      • Phosphorylation
      Purification MethodUnpurified
      UniProt Number
      UniProt SummaryFUNCTION: Specifically binds and is activated by neuregulins, NRG-2, NRG-3, heparin-binding EGF-like growth factor, betacellulin and NTAK. Interaction with these factors induces cell differentiation. Not activated by EGF, TGF-A, and amphiregulin.
      CATALYTIC ACTIVITY: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.
      SUBUNIT STRUCTURE: Homodimer or heterodimer with each of the other ERBB receptors Potential. Interacts with PDZ domains of DLG2, DLG3, DLG4 and the syntrophin SNTB2. Interacts with CBFA2T3, MUC1 and WWOX.
      SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
      TISSUE SPECIFITIY: Expressed at highest levels in brain, heart, kidney, in addition to skeletal muscle, parathyroid, cerebellum, pituitary, spleen, testis and breast. Lower levels in thymus, lung, salivary gland, and pancreas. Isoform JM-A and isoform JM-B are expressed in cerebellum, but only the isoform JM-B is expressed in the heart.
      DOMAIN: The WW-binding motifs mediate interaction with WWOX.
      PTM: Isoform JM-A is processed but not isoform JM-B. So, they respectively represent cleavable and non-cleavable forms of the receptor.
      Ligand-binding increases phosphorylation on tyrosine residues.
      SEQUENCE SIMILARITIES: Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily.
      Contains 1 protein kinase domain.
      Molecular Weight~180 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot on SKBR3 cell lysates.
      Western Blot Analysis: A 1:1,000-5,000 dilution of this antibody was used to detect Her4/ErbB-4 on SKBR3 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20ºC from date of receipt.
      Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-phospho-Her4/ErbB-4 (Tyr1258), clone EP2272AY, - 2507157 2507157
      Anti-phospho-Her4/ErbB-4 (Tyr1258), clone EP2272AY, Rabbit Monoclonal - NG1810510 NG1810510
      Anti-phospho-Her4/ErbB-4 (Tyr1258), clone EP2272AY, Rabbit Monoclonal - NG1831932 NG1831932
      Anti-phospho-Her4/ErbB-4 (Tyr1258), clone EP2272AY, Rabbit Monoclonal - NRG1692455 NRG1692455
      Anti-phospho-Her4/ErbB-4 (Tyr1258), clone EP2272AY, Rabbit Monoclonal - NRG1719639 NRG1719639
      Anti-phospho-Her4/ErbB-4 (Tyr1258), clone EP2272AY, Rabbit Monoclonal -2511125 2511125
      Anti-phospho-Her4/ErbB-4 (Tyr1258), clone EP2272AY, Rabbit Monoclonal -2556889 2556889
      Anti-phospho-Her4/ErbB-4 (Tyr1258), clone EP2272AY, Rabbit Monoclonal -2570715 2570715

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies