07-164 Anti-phospho-H2A.X (Ser139) Antibody

200 µg  
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      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      Vrt, H IP, WB, ChIP-seq Rb Purified Polyclonal Antibody
      Catalogue Number07-164
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-phospho-H2A.X (Ser139) Antibody
      Alternate Names
      • H2A histone family, member Q
      • histone 2
      • H2ac
      • histone IIa
      • histone cluster 2
      • H2ac
      Background InformationHistone H2A is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H2A is involved with the structure of the nucleosomes of the 'beads on a string' structure.
      Product Information
      • Staurosporine-treated Jurkat lysate, UV-treated HT-29 cells, doxorubicin-treated HT-29 cells.
      PresentationPurified rabbit polyclonal IgG in buffer containing 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, 0.05% sodium azide before the addition of glycerol to 30%.
      ApplicationAnti-phospho-H2A.X (Ser139) Antibody is a Rabbit Polyclonal Antibody for detection of phospho-H2A.X (Ser139) also known as histone 2 H2ac, histone IIa & has been validated in IP & WB.
      Key Applications
      • Immunoprecipitation
      • Western Blotting
      • ChIP-seq
      Application NotesImmunoprecipitation:
      A previous lot of this antibody successfully immunoprecipitated phosphorylated H2A.X as determined by an independent laboratory.
      Biological Information
      ImmunogenKLH-conjugated, synthetic peptide (CKATQA[pS]QEY) corresponding to amino acids 134-142 of human histone H2A.X. The immunizing sequence has 8 identical amino acids in yeast and mouse.
      Epitopea.a. 134-142
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      SpecificityHistone H2A.X phosphorylated at Ser139.
      Species Reactivity
      • Vertebrates
      • Human
      Species Reactivity NoteHuman; broad species cross-reactivity expected based on conservation of sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryHistones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif.
      Gene Symbol
      • HIST2H2AC
      • H2A-GL101
      • H2AFQ
      • H2A/r
      • H2A/q
      • MGC74460
      • H2A
      • Phosphorylation
      Purification MethodProtein A chromatography
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P16104 # Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C- terminal phosphorylation.
      SIZE: 143 amino acids; 15145 Da
      SUBUNIT: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. Interacts with numerous proteins required for DNA damage signaling and repair when phosphorylated on Ser-140. These include MDC1, TP53BP1, BRCA1 and the MRN complex, composed of MRE11A, RAD50, and NBN. Interaction with the MRN complex is mediated at least in part by NBN. Also interacts with DHX9/NDHII when phosphorylated on Ser-140.
      SUBCELLULAR LOCATION: Nucleus.DEVELOPMENTAL STAGE: Synthesized in G1 as well as in S-phase.
      DOMAIN: SwissProt: P16104 The [ST]-Q motif constitutes a recognition sequence for kinases from the PI3/PI4-kinase family.
      PTM: Phosphorylated on Ser-140 (to form gamma-H2AFX) in response to DNA double strand breaks (DSBs) generated by exogenous genotoxic agents and by stalled replication forks, and may also occur during meiotic recombination events and immunoglobulin class switching in lymphocytes. Phosphorylation can extend up to several thousand nucleosomes from the actual site of the DSB and may mark the surrounding chromatin for recruitment of proteins required for DNA damage signaling and repair. Widespread phosphorylation may also serve to amplify the damage signal or aid repair of persistent lesions. Phosphorylation of Ser-140 in response to ionizing radiation is mediated by both ATM and PRKDC while defects in DNA replication induce Ser-140 phosphorylation subsequent to activation of ATR and PRKDC. Dephosphorylation of Ser-140 by PP2A is required for DNA DSB repair. In meiosis, Ser-140 phosphorylation may occur at synaptonemal complexes during leptotene as an ATM-dependent response to the formation of programmed DSBs by SPO11. Ser-140 phosphorylation may subsequently occurs at unsynapsed regions of both autosomes and the XY bivalent during zygotene, downstream of ATR and BRCA1 activation. Ser-140 phosphorylation may also be required for transcriptional repression of unsynapsed chromatin and meiotic sex chromosome inactivation (MSCI), whereby the X and Y chromosomes condense in pachytene to form the heterochromatic XY-body. During immunoglobulin class switch recombination in lymphocytes, Ser-140 phosphorylation may occur at sites of DNA-recombination subsequent to activation of the activation-induced cytidine deaminase AICDA. & Monoubiquitination of Lys-120 by RING1 and RNF2/RING2 complex gives a specific tag for epigenetic transcriptional repression (By similarity).
      SIMILARITY: Belongs to the histone H2A family.
      Molecular Weight15 kDa
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by Western Blot on untreated and staurosporine treated Jurkat lysate.

      Western Blot Analysis:
      1:500 dilution of this lot detected phospho-H2A.X on 10 μg of staurosporine treated Jurkat lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C.
      Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.
      Packaging Information
      Material Size200 µg
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-phospho-H2A.X (Ser139)2474975
      Anti-phospho-H2A.X (Ser139) - 21171662117166
      Anti-phospho-H2A.X (Ser139) - 23911082391108
      Anti-phospho-H2A.X (Ser139) - 19718471971847
      Anti-phospho-H2A.X (Ser139) - 20197242019724
      Anti-phospho-H2A.X (Ser139) - 2028420284
      Anti-phospho-H2A.X (Ser139) - 21082232108223
      Anti-phospho-H2A.X (Ser139) - 21677852167785
      Anti-phospho-H2A.X (Ser139) - 2187021870
      Anti-phospho-H2A.X (Ser139) - 22785122278512


      Reference overviewApplicationSpeciesPub Med ID
      BMCC1, which is an interacting partner of BCL2, attenuates AKT activity, accompanied by apoptosis.
      Tatsumi, Y; Takano, R; Islam, MS; Yokochi, T; Itami, M; Nakamura, Y; Nakagawara, A
      Cell death & disease  6  e1607  2015

      Show Abstract
      25611382 25611382
      Analysis of the Relationships between DNA Double-Strand Breaks, Synaptonemal Complex and Crossovers Using the Atfas1-4 Mutant.
      Varas, J; Sánchez-Morán, E; Copenhaver, GP; Santos, JL; Pradillo, M
      PLoS genetics  11  e1005301  2015

      Show Abstract
      26147458 26147458
      Arabidopsis PCH2 Mediates Meiotic Chromosome Remodeling and Maturation of Crossovers.
      Lambing, C; Osman, K; Nuntasoontorn, K; West, A; Higgins, JD; Copenhaver, GP; Yang, J; Armstrong, SJ; Mechtler, K; Roitinger, E; Franklin, FC
      PLoS genetics  11  e1005372  2015

      Show Abstract
      26182244 26182244
      Long-term quiescent fibroblast cells transit into senescence.
      Marthandan, S; Priebe, S; Hemmerich, P; Klement, K; Diekmann, S
      PloS one  9  e115597  2014

      Show Abstract
      Western Blotting25531649 25531649
      STAG3-mediated stabilization of REC8 cohesin complexes promotes chromosome synapsis during meiosis.
      Fukuda, T; Fukuda, N; Agostinho, A; Hernández-Hernández, A; Kouznetsova, A; Höög, C
      The EMBO journal  33  1243-55  2014

      Show Abstract
      24797475 24797475
      BRCA1 pathway function in basal-like breast cancer cells.
      Hill, SJ; Clark, AP; Silver, DP; Livingston, DM
      Molecular and cellular biology  34  3828-42  2014

      Show Abstract
      25092866 25092866
      Double-strand break repair deficiency in NONO knockout murine embryonic fibroblasts and compensation by spontaneous upregulation of the PSPC1 paralog.
      Li, S; Li, Z; Shu, FJ; Xiong, H; Phillips, AC; Dynan, WS
      Nucleic acids research  42  9771-80  2014

      Show Abstract
      Immunofluorescence25100870 25100870
      Salinomycin induces apoptosis and senescence in breast cancer: upregulation of p21, downregulation of survivin and histone H3 and H4 hyperacetylation.
      Al Dhaheri, Y; Attoub, S; Arafat, K; Abuqamar, S; Eid, A; Al Faresi, N; Iratni, R
      Biochimica et biophysica acta  3121-35  2013

      Show Abstract
      Immunofluorescence23352703 23352703
      Targeting telomere-containing chromosome ends with a near-infrared femtosecond laser to study the activation of the DNA damage response and DNA damage repair pathways.
      Silva, BA; Stambaugh, JR; Berns, MW
      Journal of biomedical optics  18  095003  2013

      Show Abstract
      24064949 24064949
      The histone methyltransferase MMSET regulates class switch recombination.
      Pei, H; Wu, X; Liu, T; Yu, K; Jelinek, DF; Lou, Z
      Journal of immunology (Baltimore, Md. : 1950)  190  756-63  2013

      Show Abstract
      23241889 23241889

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