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MABS1187 | Anti-phospho-GADS (Thr262) Antibody, clone 6E11.2

MABS1187
100 μg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H WB M Purified Monoclonal Antibody
      Description
      Catalogue NumberMABS1187
      DescriptionAnti-phospho-GADS (Thr262) Antibody, clone 6E11.2
      Alternate Names
      • GRB2-related adapter protein 2, Thr262 phosphorylated
      • Adapter protein GRID, Thr262 phosphorylated
      • GRAP-2, Thr262 phosphorylated
      • GRB-2-like protein, Thr262 phosphorylated
      • Grb2-related adapter protein downstream of Shc, Thr262 phosphorylated
      • GRB2L, Thr262 phosphorylated
      • GRBLG, Thr262 phosphorylated
      • GRBX, Thr262 phosphorylated
      • Grf-40, Thr262 phosphorylated
      • Grf40 adapter protein, Thr262 phosphorylated
      • Growth factor receptor-binding protein, Thr262 phosphorylated
      • Hematopoietic cell-associated adapter protein GrpL, Thr262 phosphorylated
      • P38, Thr262 phosphorylated
      • Protein GADS, Thr262 phosphorylated
      • SH3-SH2-SH3 adapter Mona, Thr262 phosphorylated
      Background InformationGRB2-related adapter protein 2 (UniProt O75791; also known as Adapter protein GRID, GRAP-2, GRB-2-like protein, Grb2-related adapter protein downstream of Shc, GRB2L, GRBLG, GRBX, Grf-40, Grf40 adapter protein, Growth factor receptor-binding protein, Hematopoietic cell-associated adapter protein GrpL, P38, Protein GADS, SH3-SH2-SH3 adapter Mona) is encoded by the GRAP2 (also known as GADS, GRB2L, GRID) gene (Gene ID 9402) in human. GADS (Grb2-related adapter protein downstream of Shc), GRB2 and GRAP constitute the GRB2 family of adapter proteins characterized by a central SH2 domain (a.a. 58-149 of GADS) flanked between two SH3 domains (a.a. 1-56, 271-330 of GADS). Although the SH2 domains of all three members have similar binding specificities, the C-terminal SH3 domain of GADS possesses unique binding specificity that allows for its interaction with the adapter protein SLP-76 (SH2 domain-containing leukocyte protein of 76 kDa). The GADS-SLP-76 complex is recruited to tyrosine-phosphorylated LAT (linker for activation of T cells) via GADS SH2 domain, allowing the assembly of signaling complexes critical to T-cell activation. In addition, evidences indicate that GADS plays a critical role in the development of BCR-ABL-mediated lymphoid disease. The oncogenic Bcr-Abl fusion protein is shown to bind GADS in a phosphotyrosine-dependent manner and GADS is expressed in a subset of CML cell lines and B-ALL patient samples, where it associates with both BCR-ABL and SLP-76.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-phospho-GADS (Thr262) Antibody, clone 6E11.2 is an antibody against phospho-GADS for use in Western Blotting.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to a human GADS sequence containing phosphorylated Thr262.
      EpitopepThr262.
      Clone6E11.2
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 6E11.2 detects GADS phosphorylated on Thr262. The corresponding phosphorylation site in human GADS spliced isoform 2 is Thr149 (UniProt O75791-2).
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • GRAP2
      • GADS
      • GRB2L
      • GRID
      Modifications
      • Phosphorylation
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~40 kDa observed. 37.91 kDa (isoform 1) and 24.38 kDa (isoform 2) calculated. Uncharacterized band(s) may appear in some lysates.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in Jurkat cell lysate.

      Western Blotting Analysis: 0.5 µg/mL of this antibody detected GADS Thr262 phosphorylation induction in 10 µg lysate from Calyculin A- and TNFα-treated Jurkat cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies