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06-1433 | Anti-phospho-FGFR-1 (Tyr653/Tyr654) Antibody

06-1433
100 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, R, Ch, B, Xn WB, Cell Function Assay Rb Affinity Purified Polyclonal Antibody
      Description
      Catalogue Number06-1433
      DescriptionAnti-phospho-FGFR-1 (Tyr653/Tyr654) Antibody
      Alternate Names
      • Basic fibroblast growth factor receptor 1
      • FGFR-1
      • bFGF-R-1
      • Fms-like tyrosine kinase 2
      • FLT-2
      • Proto-oncogene c-Fgr
      • CD331
      Background InformationFGFR-1 (fibroblast growth factor receptor 1) is a member of the basic fibroblast growth factor family of proteins and is thought to be important in the induction and propagation of benign prostatic hyperplasia. A shortage of FGFR-1 causes death post-implantation and has been linked to neurofascin and neurite outgrowth. FGFR-1 expression has recently been found to be increased in patients with renal cell carcinoma and FGFR-1 may also have a role to play in early limb formation.
      References
      Product Information
      FormatAffinity Purified
      Control
      • Untreated and lambda phosphatase-treated HEK293T cells transfected with FGFR-1
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationDetect phospho-FGFR-1 (Tyr653/Tyr654) using this Anti-phospho-FGFR-1 (Tyr653/Tyr654) Antibody validated for use in WB, Cell Function Assay.
      Key Applications
      • Western Blotting
      • Cell Function Assay
      Application NotesPeptide Inhibition Assay Analysis: 0.2 µg/mL from a representative lot peptide blocked in HEK293T cells transfected with FGFR-1.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to human FGFR-1 phosphorylated at Tyr653/Tyr654.
      EpitopePhosphorylated Tyr653/Tyr654
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityThis antibody recognizes FGFR-1 phosphorylated at Tyr653/Tyr654.
      Species Reactivity
      • Human
      • Mouse
      • Rat
      • Chicken
      • Bovine
      • Xenopus
      Species Reactivity NoteDemonstrated to react with Human.
      Predicted to react with Mouse, Rat, Chicken, Bovine, Xenopus, and Zebra Fish based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is a member of the fibroblast growth factor receptor (FGFR) family, where amino acid sequence is highly conserved between members and throughout evolution. FGFR family members differ from one another in their ligand affinities and tissue distribution. A full-length representative protein consists of an extracellular region, composed of three immunoglobulin-like domains, a single hydrophobic membrane-spanning segment and a cytoplasmic tyrosine kinase domain. The extracellular portion of the protein interacts with fibroblast growth factors, setting in motion a cascade of downstream signals, ultimately influencing mitogenesis and differentiation. This particular family member binds both acidic and basic fibroblast growth factors and is involved in limb induction. Mutations in this gene have been associated with Pfeiffer syndrome, Jackson-Weiss syndrome, Antley-Bixler syndrome, osteoglophonic dysplasia, and autosomal dominant Kallmann syndrome 2. Chromosomal aberrations involving this gene are associated with stem cell myeloproliferative disorder and stem cell leukemia lymphoma syndrome. Alternatively spliced variants which encode different protein isoforms have been described; however, not all variants have been fully characterized. [provided by RefSeq].
      Gene Symbol
      • FGFR1
      • FGFBR
      • FLG
      • FLT2
      Modifications
      • Phosphorylation
      • Phosphorylation
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFUNCTION: Receptor for fibroblast growth factors FGF2 and FGF1. Receptor for FGF23 in the presence of KL (By similarity). Promotes mitogenesis in response to fibroblast growth factors. Activates PLCG1.

      CATALYTIC ACTIVITY: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.

      SUBUNIT STRUCTURE: Homodimer. Interacts with KLB (By similarity). Interacts with KL and FGF23 (By similarity). Interacts with SHB and GRB10. Interacts with PLCG1 (via SH2 domains). Interacts with KAL1; this interaction does not interfere with FGF2-binding to FGFR1, but prevents binding of heparin-bound FGF2. Interacts with SOX2 and SOX3 (By similarity). SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.

      TISSUE SPECIFICITY: Detected in astrocytoma, neuroblastoma and adrenal cortex cell lines. Some isoforms are detected in foreskin fibroblast cell lines, however isoform 17, isoform 18 and isoform 19 are not detected in these cells.

      PTM: Binding of FGF1 and heparin promotes autophosphorylation on tyrosine residues and activation of the receptor.

      INVOLVEMENT IN DISEASE: Defects in FGFR1 are a cause of Pfeiffer syndrome (PS) [MIM:101600]; also known as acrocephalosyndactyly type V (ACS5). PS is characterized by craniosynostosis (premature fusion of the skull sutures) with deviation and enlargement of the thumbs and great toes, brachymesophalangy, with phalangeal ankylosis and a varying degree of soft tissue syndactyly.

      Defects in FGFR1 are a cause of idiopathic hypogonadotropic hypogonadism (IHH) [MIM:146110]. IHH is defined as a deficiency of the pituitary secretion of follicle-stimulating hormone and luteinizing hormone, which results in the impairment of pubertal maturation and of reproductive function.

      Defects in FGFR1 are the cause of Kallmann syndrome type 2 (KAL2) [MIM:147950]; also known as hypogonadotropic hypogonadism and anosmia. Anosmia or hyposmia is related to the absence or hypoplasia of the olfactory bulbs and tracts. Hypogonadism is due to deficiency in gonadotropin-releasing hormone and probably results from a failure of embryonic migration of gonadotropin-releasing hormone-synthesizing neurons. In some cases, midline cranial anomalies (cleft lip/palate and imperfect fusion) are present and anosmia may be absent or inconspicuous.

      Defects in FGFR1 are the cause of osteoglophonic dysplasia (OGD) [MIM:166250]; also known as osteoglophonic dwarfism. OGD is characterized by craniosynostosis, prominent supraorbital ridge, and depressed nasal bridge, as well as by rhizomelic dwarfism and nonossifying bone lesions. Inheritance is autosomal dominant.

      Defects in FGFR1 are the cause of trigonocephaly non-syndromic (TRICEPH) [MIM:190440]; also known as metopic craniosynostosis. The term trigonocephaly describes the typical keel-shaped deformation of the forehead resulting from premature fusion of the frontal suture. Trigonocephaly may occur also as a part of a syndrome.

      Note=A chromosomal aberration involving FGFR1 may be a cause of stem cell leukemia lymphoma syndrome (SCLL). Translocation t(8;13)(p11;q12) with ZMYM2. SCLL usually presents as lymphoblastic lymphoma in association with a myeloproliferative disorder, often accompanied by pronounced peripheral eosinophilia and/or prominent eosinophilic infiltrates in the affected bone marrow.

      Note=A chromosomal aberration involving FGFR1 may be a cause of stem cell myeloproliferative disorder (MPD). Translocation t(6;8)(q27;p11) with FGFR1OP. Insertion ins(12;8)(p11;p11p22) with FGFR1OP2. MPD is characterized by myeloid hyperplasia, eosinophilia and T-cell or B-cell lymphoblastic lymphoma. In general it progresses to acute myeloid leukemia. The fusion proteins FGFR1OP2-FGFR1, FGFR1OP-FGFR1 or FGFR1-FGFR1OP may exhibit constitutive kinase activity and be responsible for the transforming activity.

      Note=A chromosomal aberration involving FGFR1 may be a cause of stem cell myeloproliferative disorder (MPD). Translocation t(8;9)(p12;q33) with CEP110. MPD is characterized by myeloid hyperplasia, eosinophilia and T-cell or B-cell lymphoblastic lymphoma. In general it progresses to acute myeloid leukemia. The fusion protein CEP110-FGFR1 is found in the cytoplasm, exhibits constitutive kinase activity and may be responsible for the transforming activity.

      SEQUENCE SIMILARITIES: Belongs to the protein kinase superfamily. Tyr protein kinase family. Fibroblast growth factor receptor subfamily.

      Contains 3 Ig-like C2-type (immunoglobulin-like) domains.

      Contains 1 protein kinase domain.
      Molecular Weight~145 kDa observed.
      FGFR-1 has 19 isoforms and eight potential glycosylation sites that increase the expected size to 120 kDa for the immature form and 145 kDa for the mature form of the receptor (Hisaoka, K., et al. (2011). JBC. 286(24):21118-21128).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in untreated and lambda phosphatase-treated HEK293T cells transfected with FGFR1.

      Western Blot Analysis: 0.2 µg/mL of this antibody detected FGFR-1 in 10 µg of untreated and lambda phosphatase-treated HEK293T cells transfected with FGFR1.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-phospho-FGFR-1 (Tyr653/Tyr654) 2475044
      Anti-phospho-FGFR-1 (Tyr653/Tyr654) - 2147125 2147125
      Anti-phospho-FGFR-1 (Tyr653/Tyr654) - 2026347 2026347
      Anti-phospho-FGFR-1 (Tyr653/Tyr654) - 2219379 2219379
      Anti-phospho-FGFR-1 (Tyr653/Tyr654) - 2289174 2289174
      Anti-phospho-FGFR-1 (Tyr653/Tyr654) - 2520359 2520359
      Anti-phospho-FGFR-1 (Tyr653/Tyr654) - NRG1915401 NRG1915401
      Anti-phospho-FGFR-1 (Tyr653/Tyr654) -2576380 2576380
      Anti-phospho-FGFR-1 (Tyr653/Tyr654) -2730011 2730011
      Anti-phospho-FGFR-1 (Tyr653/Tyr654) -2787243 2787243

      References

      Reference overviewPub Med ID
      LTBP-2 Has a Single High-Affinity Binding Site for FGF-2 and Blocks FGF-2-Induced Cell Proliferation.
      Menz, C; Parsi, MK; Adams, JR; Sideek, MA; Kopecki, Z; Cowin, AJ; Gibson, MA
      PloS one 10 e0135577 2015

      Show Abstract
      26263555 26263555
      Identification of distinct ChAT⁺ neurons and activity-dependent control of postnatal SVZ neurogenesis.
      Paez-Gonzalez, P; Asrican, B; Rodriguez, E; Kuo, CT
      Nature neuroscience 17 934-42 2014

      Show Abstract
      24880216 24880216

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies