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05-1004 | Anti-phospho-EGFR (Tyr1173) Antibody, clone 3G3.2

100 µg  
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      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H WB, FC, IHC, ICC M Purified Monoclonal Antibody
      Catalogue Number05-1004
      DescriptionAnti-phospho-EGFR (Tyr1173) Antibody, clone 3G3.2
      Alternate Names
      • C-type lectin domain family 14, member A
      • ClECT and EGF-like domain containing protein
      • Epidermal growth factor receptor 5
      • chromosome 14 open reading frame 27
      Background InformationThe epidermal growth factor receptor (EGFR; ErbB-1; HER1 in humans) is the cell-surface receptor for members of the epidermal growth factor family (EGF-family) of extracellular protein ligands. The epidermal growth factor receptor is a member of the ErbB family of receptors, a subfamily of four closely related receptor tyrosine kinases: EGFR (ErbB-1), HER2/c-neu (ErbB-2), Her 3 (ErbB-3) and Her 4 (ErbB-4). Mutations affecting EGFR expression or activity could result in cancer. EGFR (epidermal growth factor receptor) exists on the cell surface and is activated by binding of its specific ligands, including epidermal growth factor and transforming growth factor α (TGFα). EGFR dimerization stimulates its intrinsic intracellular protein-tyrosine kinase activity. As a result, autophosphorylation of five tyrosine (Y) residues in the C-terminal domain of EGFR occurs.
      Product Information
      • EGF treated A431 cell lysate
      PresentationPurified rabbit ascites containing 0.1 M Tris-glycine, pH 7.4, 150 mM NaCl and 0.05% sodium azide.
      ApplicationDetect phospho-EGFR (Tyr1173) using this Anti-phospho-EGFR (Tyr1173) Antibody, clone 3G3.2 validated for use in WB, FC, IH & IC.
      Key Applications
      • Western Blotting
      • Flow Cytometry
      • Immunohistochemistry
      • Immunocytochemistry
      Application NotesFlow Cytometry Analysis: A431 cells stained using 10 ug/mL of anti-phospho -EGFR (Tyr1173).
      Immunocytochemistry Analysis: A 1:500 dilution of this antibody
      detected anti-phospho-EGFR (Tyr1173) in EGF treated A431
      Immunohistochemistry Analysis: A 1:100 dilution of this detected anti-phospho-EGFR (Tyr1173) in serous ovarian cancer.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to residues surrounding Tyr1173 of EGFR.
      EpitopePhosphorylated Tyr1173
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      SpecificityThis antibody recognizes phosphorylated EGFR (Tyr1173).
      Species Reactivity
      • Human
      Species Reactivity NoteProven to react with human.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer.
      Gene Symbol
      • C14orf27
      • CEG1
      • EGFR-5
      • EGFR5
      • OTTHUMP00000178873
      • Phosphorylation
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummarySUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein Potential.
      SEQUENCE SIMILARITIES: Contains 1 C-type lectin domain.
      Contains 1 EGF-like domain.
      Molecular Weight175 kDa
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in EGF treated and untreated A431 cell lysate.
      Western Blot Analysis: 0.5 µg/mL of this antibody detected phospho-EGFR (Tyr1173) on 15 µg of EGF treated A431 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain refrigerated at 2-8°C for 1 year from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-phospho-EGFR (Tyr1173), clone 3G3.2 - 2393900 2393900
      Anti-phospho-EGFR (Tyr1173), -2580673 2580673
      Anti-phospho-EGFR (Tyr1173), clone 3G3.2 - 1977638 1977638
      Anti-phospho-EGFR (Tyr1173), clone 3G3.2 - 2324383 2324383
      Anti-phospho-EGFR (Tyr1173), clone 3G3.2 - NG1715103 NG1715103
      Anti-phospho-EGFR (Tyr1173), clone 3G3.2 - NG1884458 NG1884458
      Anti-phospho-EGFR (Tyr1173), clone 3G3.2 - NRG1633602 NRG1633602