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07-818 | Anti-phospho-EGFR (Tyr1086) Antibody

100 µL  
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      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H WB Rb Affinity Purified Polyclonal Antibody
      Catalogue Number07-818
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-phospho-EGFR (Tyr1086) Antibody
      Product Information
      FormatAffinity Purified
      ApplicationAnti-phospho-EGFR (Tyr1086) Antibody detects level of phospho-EGFR (Tyr1086) & has been published & validated for use in WB.
      Key Applications
      • Western Blotting
      Application NotesImmunoblot Analysis: A 1:1000 dilution of this lot detected phospho-EGFR (Tyr1086) in RIPA lysates from EGF stimulated A431 cells.
      Biological Information
      ImmunogenPeptide corresponding to amino acid region encompassing the human, mouse, and rat phospho-EGFR (Tyr1086)
      SpecificityRecognizes phospho-EGFR (Tyr1086)
      Species Reactivity
      • Human
      Species Reactivity NotePredicted to cross-react with mouse and rat based on sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • EGFR
      • ERBB1
      • ERBB
      • mENA
      • Phosphorylation
      Purification MethodImmunoAffinity Purified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P00533 # Isoform 2/truncated isoform may act as an antagonist.
      SIZE: 1210 amino acids; 134277 Da
      SUBUNIT: Binds RIPK1. CBL interacts with the autophosphorylated C- terminal tail of the EGF receptor. Part of a complex with ERBB2 and either PIK3C2A or PIK3C2B. The autophosphorylated form interacts with PIK3C2B, maybe indirectly. Interacts with PELP1.
      SUBCELLULAR LOCATION: Cell membrane; Single-pass type I membrane protein. & Isoform 2: Secreted.
      TISSUE SPECIFICITY: Expressed in placenta. Isoform 2 is also expressed in ovarian cancers.
      PTM: Phosphorylation of Ser-695 is partial and occurs only if Thr- 693 is phosphorylated. & Monoubiquitinated and polyubiquitinated upon EGF stimulation; which does not affect tyrosine kinase activity or signaling capacity but may play a role in lysosomal targeting. Polyubiquitin linkage is mainly through 'Lys-63', but linkage through 'Lys-48', 'Lys-11' and 'Lys-29' also occur.DISEASE:SwissProt: P00533 # Defects in EGFR are associated with lung cancer [MIM:211980].
      SIMILARITY: SwissProt: P00533 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily. & Contains 1 protein kinase domain.
      MISCELLANEOUS: Binding of EGF to the receptor leads to dimerization, internalization of the EGF-receptor complex, induction of the tyrosine kinase activity, stimulation of cell DNA synthesis, and cell proliferation.
      Molecular Weight185 kDa
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by immunoblot.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 2 years at -20°C from date of shipment.
      Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerolcontaining solutions to become frozen during storage.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-phospho-EGFR (Tyr1086) (Affinity purified Rabbit polyclonal IgG) - 2379278 2379278
      Anti-phospho-EGFR (Tyr1086) (Affinity purified Rabbit polyclonal IgG) - 2446295 2446295
      Anti-phospho-EGFR (Tyr1086) (Affinity purified Rabbit polyclonal IgG) - 2295394 2295394
      Anti-phospho-EGFR (Tyr1086) (Affinity purified Rabbit polyclonal IgG) - NG1915867 NG1915867
      Anti-phospho-EGFR (Tyr1086) (Affinity purified Rabbit polyclonal IgG) -2506279 2506279
      Anti-phospho-EGFR (Tyr1086) -2625206 2625206
      anti-phospho-EGFR (Tyr1086) - 0604027380 0604027380


      Reference overviewApplicationPub Med ID
      Changes in retinal cell fate induced by overexpression of EGF receptor
      Lillien, L.
      Nature, 377:158-62 (1995) 1995

      Immunohistochemistry (Tissue)7675083 7675083
      Amphiregulin induces tyrosine phosphorylation of the epidermal growth factor receptor and p185erbB2. Evidence that amphiregulin acts exclusively through the epidermal growth factor receptor at the surface of human epithelial cells.
      Johnson, G R, et al.
      J. Biol. Chem., 268: 2924-31 (1993) 1993

      Show Abstract
      7679104 7679104
      Polymorphonuclear leukocytes-mediated lysis of A431 cells induced by IgG1 mouse anti-epidermal growth factor receptor monoclonal antibodies.
      Kawamoto, T, et al.
      In Vitro Cell. Dev. Biol., 28A: 782-6 (1992) 1992

      Show Abstract
      1483969 1483969
      Cytotoxic properties of DAB486EGF and DAB389EGF, epidermal growth factor (EGF) receptor-targeted fusion toxins.
      Shaw, J P, et al.
      J. Biol. Chem., 266: 21118-24 (1991) 1991

      Show Abstract
      1939154 1939154

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      Life Science Research > Antibodies and Assays > Primary Antibodies