A part of MilliporeSigma

04-286 | Anti-phospho-EGFR (Tyr1086) Antibody, clone 8B8

100 µg  
Retrieving price...
Price could not be retrieved
Minimum Quantity needs to be mulitiple of
Upon Order Completion More Information
You Saved ()
Request Pricing
Limited AvailabilityLimited Availability
Limited Quantities Available
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service

      Special Offers


      Contact Customer Service

      Click To Print This Page


      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H ELISA, WB M Purified Monoclonal Antibody
      Catalogue Number04-286
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-phospho-EGFR (Tyr1086) Antibody, clone 8B8
      Alternate Names
      • ERBB
      • ERBB1
      Product Information
      • Includes pervanadate treated A431 cell lysate as a positive control
      ApplicationAnti-phospho-EGFR (Tyr1086) Antibody, clone 8B8 is an antibody against phospho-EGFR (Tyr1086) for use in ELISA & WB.
      Key Applications
      • ELISA
      • Western Blotting
      Biological Information
      ImmunogenKLH-conjugated synthetic peptide encompassing the surrounding amino acids of Tyr 1086 in human EGFR.
      SpecificityRecognizes phosphorylated EGFR on Tyrosine 1086.
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • EGFR
      • ERBB1
      • ERBB
      • mENA
      • Phosphorylation
      Purification MethodSubsequent thiophilic adsorption and size exclusion chromatography
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P00533 # Isoform 2/truncated isoform may act as an antagonist.
      SIZE: 1210 amino acids; 134277 Da
      SUBUNIT: Binds RIPK1. CBL interacts with the autophosphorylated C- terminal tail of the EGF receptor. Part of a complex with ERBB2 and either PIK3C2A or PIK3C2B. The autophosphorylated form interacts with PIK3C2B, maybe indirectly. Interacts with PELP1.
      SUBCELLULAR LOCATION: Cell membrane; Single-pass type I membrane protein. & Isoform 2: Secreted.
      TISSUE SPECIFICITY: Expressed in placenta. Isoform 2 is also expressed in ovarian cancers.
      PTM: Phosphorylation of Ser-695 is partial and occurs only if Thr- 693 is phosphorylated. & Monoubiquitinated and polyubiquitinated upon EGF stimulation; which does not affect tyrosine kinase activity or signaling capacity but may play a role in lysosomal targeting. Polyubiquitin linkage is mainly through 'Lys-63', but linkage through 'Lys-48', 'Lys-11' and 'Lys-29' also occur.DISEASE:SwissProt: P00533 # Defects in EGFR are associated with lung cancer [MIM:211980].
      SIMILARITY: SwissProt: P00533 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily. & Contains 1 protein kinase domain.
      MISCELLANEOUS: Binding of EGF to the receptor leads to dimerization, internalization of the EGF-receptor complex, induction of the tyrosine kinase activity, stimulation of cell DNA synthesis, and cell proliferation.
      Molecular Weight180 kDa
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by immunoblot.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions2 years at -20°C from date of shipment
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information


      Certificates of Analysis

      TitleLot Number
      Anti-phospho-EGFR (Tyr1086), clone 8B8 - 2139975 2139975
      Anti-phospho-EGFR (Tyr1086), clone 8B8 - 0188S0101 0188S0101
      Anti-phospho-EGFR (Tyr1086), clone 8B8 - 2315608 2315608


      Reference overviewApplicationPub Med ID
      Tumor-initiating cells of HER2-positive carcinoma cell lines express the highest oncoprotein levels and are sensitive to trastuzumab.
      Magnifico, Alessandra, et al.
      Clin. Cancer Res., 15: 2010-21 (2009) 2009

      Show Abstract
      Immunoblotting (Western)19276287 19276287
      Modulation of survival signaling pathways and persistence of the genotoxic stress as a basis for the synergistic interaction between the atypical retinoid ST1926 and the epidermal growth factor receptor inhibitor ZD1839
      Zanchi, Chiara, et al
      Cancer Res, 65:2364-72 (2005) 2005

      15781651 15781651
      Identification of a novel autophosphorylation site (P4) on the epidermal growth factor receptor
      Hsuan, J J, et al
      Biochem J, 262:659-63 (1989) 1989

      2803273 2803273

      Related Products & Applications

      Related Products

      Catalog Number Description  
      04-281 Anti-phospho-EGFR (Thr669) Antibody, clone 5F10 Show Pricing & Availability
      04-282 Anti-phospho-EGFR (Thr654) Antibody, clone 3F2 Show Pricing & Availability
      04-283 Anti-phospho-EGFR (Tyr845) Antibody, clone 12A3 Show Pricing & Availability
      04-290 Anti-EGFR (CT) Antibody, clone 13G8 Show Pricing & Availability
      04-337 Anti-EGFR Antibody, clone E114, rabbit monoclonal Show Pricing & Availability
      04-338 Anti-EGFR Antibody, rabbit monoclonal Show Pricing & Availability
      04-339 Anti-phospho-EGFR (Tyr1068) Antibody, clone EP774Y, rabbit monoclonal Show Pricing & Availability
      04-341 Anti-phospho-EGFR (Tyr1173) Antibody, rabbit monoclonal Show Pricing & Availability
      05-101 Anti-EGFR Antibody, neutralizing, clone LA1 Show Pricing & Availability
      05-104 Anti-EGFR Antibody, clone LA22 Show Pricing & Availability
      05-1047 Anti-EGFR (cytoplasmic domain) Antibody, clone 8G6.2 Show Pricing & Availability
      05-1128 Anti-active-EGFR (intracellular) Antibody, clone 74 Show Pricing & Availability
      05-483 Anti-phospho-EGFR (Tyr1173) Antibody, clone 9H2 Show Pricing & Availability
      05-484 Anti-EGFR (non-phospho-Tyr1173) Antibody, clone 20G3 Show Pricing & Availability
      06-847 Anti-EGFR Antibody Show Pricing & Availability
      07-1024 Anti-EGFR Antibody, cytoplasmic domain Show Pricing & Availability
      07-715 Anti-phospho-EGFR (Tyr1069) Antibody Show Pricing & Availability
      07-818 Anti-phospho-EGFR (Tyr1086) Antibody Show Pricing & Availability
      07-819 Anti-phospho-EGFR (Tyr1148) Antibody Show Pricing & Availability
      07-820 Anti-phospho-EGFR (Tyr845) Antibody Show Pricing & Availability
      07-821 Anti-phospho-EGFR (Tyr992) Antibody Show Pricing & Availability
      16-244 Anti-phospho-EGFR (Tyr1173) Antibody, clone 9H2, Alexa Fluor® 488 Show Pricing & Availability
      16-246 Anti-EGFR Antibody, neutralizing, clone LA1, Alexa Fluor® 488 Show Pricing & Availability
      16-247 Anti-EGFR Antibody, neutralizing, clone LA1, Alexa Fluor® 555 Show Pricing & Availability
      FCABS254F Milli-Mark™ Anti-EGFR-FITC Antibody Show Pricing & Availability
      FCMAB129CP Milli-Mark Anti-EGFR-PerCP Antibody, clone LA22 Show Pricing & Availability
      MABF119 Anti-EGFR Antibody, clone 528 (Azide-free) Show Pricing & Availability
      MABF120 Anti-EGFR Antibody, clone 225 (Azide-free) Show Pricing & Availability

      Product Families

      View Gene Related Products for this Target


      Life Science Research > Antibodies and Assays > Primary Antibodies