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09-295 | Anti-phospho-Calmodulin (Tyr99) Antibody

100 µL  
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      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, B, Eq, R WB Rb Affinity Purified Polyclonal Antibody
      Catalogue Number09-295
      DescriptionAnti-phospho-Calmodulin (Tyr99) Antibody
      Alternate Names
      • calmodulin 1
      • calmodulin 1 (phosphorylase kinase, delta)
      • phosphorylase kinase, delta subunit
      Background InformationCalmodulin (CaM) is a ubiquitous, calcium-binding protein that can bind to and regulate a multitude of different protein targets, thereby affecting many different cellular functions.
      CaM mediates processes such as inflammation, metabolism, apoptosis, muscle contraction, intracellular movement, short-term and long-term memory, nerve growth and the immune response. CaM is expressed in many cell types and can have different subcellular locations, including the cytoplasm, within organelles, or associated with the plasma or organelle membranes. Many of the proteins that CaM binds are unable to bind calcium themselves, and as such use CaM as a calcium sensor and signal transducer. CaM undergoes a conformational change upon binding to calcium, which enables it to bind to specific proteins for a specific response. CaM can bind up to four calcium ions, and can undergo post-translational modifications, such as phosphorylation, acetylation, methylation and proteolytic cleavage, each of which can potentially modulate its actions.
      Product Information
      FormatAffinity Purified
      • EGF treated and untreated HeLa cell lysate
      PresentationAffinity purified rabbit serum in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
      ApplicationAnti-phospho-Calmodulin (Tyr99) Antibody detects level of phospho-Calmodulin (Tyr99) & has been published & validated for use in WB.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to human phosphorylated Calmodulin at and around Tyr99.
      EpitopePhosphorylated Tyr99
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      SpecificityThis antibody recognizes phosphorylated Calmodulin at Tyr99.
      Species Reactivity
      • Human
      • Mouse
      • Bovine
      • Horse
      • Rat
      Species Reactivity NoteReacts with human and mouse. Predicted to react with horse, rat and cow based on sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryCalmodulin is the archetype of the family of calcium-modulated proteins of which nearly 20 members have been found. They are identified by their occurrence in the cytosol or on membranes facing the cytosol and by a high affinity for calcium. Calmodulin contains 149 amino acids and has 4 calcium-binding domains. Its functions include roles in growth and the cell cycle as well as in signal transduction and the synthesis and release of neurotransmitters.
      Gene Symbol
      • CALM1
      • CALML2
      • CALML2
      • CALM3
      • CALM
      • CaM
      • CALM2
      • CAM2
      • CAMC
      • CAM3
      • DD132
      • CAMIII
      • PHKD
      • CAMI
      • CAMB
      • CAM
      • CAM1
      • Phosphorylation
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFUNCTION: Calmodulin mediates the control of a large number of enzymes and other proteins by Ca2+. Among the enzymes to be stimulated by the calmodulin-Ca2+ complex are a number of protein kinases and phosphatases. Together with CEP110 and centrin, is involved in a genetic pathway that regulates the centrosome cycle and progression through cytokinesis.

      SUBUNIT STRUCTURE: Interacts with MYO1C By similarity. Interacts with CEP97, CEP110, TTN/titin and SRY.

      SUBCELLULAR LOCATION: Spindle. Note= Distributed throughout the cell during interphase, but during mitosis becomes dramatically localized to the spindle poles and the spindle microtubules.

      PTM: Ubiquitination results in a strongly decreased activity By similarity.

      Phosphorylation results in a decreased activity By similarity.

      MISCELLANEOUS: This protein has four functional calcium-binding sites.

      SEQUENCE SIMILARITIES: Belongs to the calmodulin family.

      Contains 4 EF-hand domains.

      Sequence caution The sequence CAA36839.1 differs from that shown. Reason: Erroneous gene model prediction.
      Molecular Weight17 kDa
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in HeLa cell lysate.

      Western Blot Analysis: 0.5 µg/mL of this antibody detected Calmodulin phosphorylated at Tyr99 in EGF treated and untreated HeLa cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-phospho-Calmodulin (Tyr99) - 2051952 2051952
      Anti-phospho-Calmodulin (Tyr99) - 2502948 2502948
      Anti-phospho-Calmodulin (Tyr99) - NRG1659211 NRG1659211


      Reference overviewApplicationPub Med ID
      PTPRZ1 regulates calmodulin phosphorylation and tumor progression in small-cell lung carcinoma.
      Makinoshima, H; Ishii, G; Kojima, M; Fujii, S; Higuchi, Y; Kuwata, T; Ochiai, A
      BMC cancer 12 537 2012

      Show Abstract
      23170925 23170925
      Significance of calcium binding, tyrosine phosphorylation, and lysine trimethylation for the essential function of calmodulin in vertebrate cells analyzed in a novel gene replacement system.
      Panina, S; Stephan, A; la Cour, JM; Jacobsen, K; Kallerup, LK; Bumbuleviciute, R; Knudsen, KV; Sánchez-González, P; Villalobo, A; Olesen, UH; Berchtold, MW
      The Journal of biological chemistry 287 18173-81 2012

      Show Abstract
      Western Blotting22493455 22493455

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      Life Science Research > Antibodies and Assays > Primary Antibodies