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07-507 | Anti-phospho-BMK1/Erk5 (Thr218/Tyr220) Antibody

200 µL  
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      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, R, B WB, PIA Rb Affinity Purified Polyclonal Antibody
      Catalogue Number07-507
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-phospho-BMK1/Erk5 (Thr218/Tyr220) Antibody
      Product Information
      FormatAffinity Purified
      • Sorbitol-treated HeLa cells
      PresentationAffinity purified immunoglobulin in 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl, with 0.05% sodium azide.
      ApplicationThis Anti-phospho-BMK1/Erk5 (Thr218/Tyr220) Antibody is validated for use in WB for the detection of phospho-BMK1/Erk5 (Thr218/Tyr220).
      Key Applications
      • Western Blotting
      • Peptide Inhibition Assay
      Application NotesPeptide Inhibition Analysis:
      HeLa cell lysate, HeLa cell lysate+ non-phosphorylated protein, and HeLa cell lysate + phosphorylated peptide were probed with Cat. No. 07-507, Anti-phospho-BMK 1/ERK5 (Thr218/Tyr220) (1µg/mL(1:1000) dilution). Proteins were visualized using a Donkey Anti-Rabbit IgG secondary antibody conjugated to HRP and a chemiluminesence detection system.

      Arrow indicates BMK1/ERK5 (~115 kDa) on HeLa cell lysate & HeLa cell lysate + non-phosphorylated peptide. No band is present on the HeLa cell lysate + phosphorylated peptide indicating successful peptide inhibition of the antibody.
      Biological Information
      ImmunogenKLH-conjugated, synthetic peptide corresponding to amino acids 213-224 of human BMK1/Erk5
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      SpecificityRecognizes phospho-BMK1/Erk5 (Thr218, Tyr220).
      Species Reactivity
      • Human
      • Mouse
      • Rat
      • Bovine
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is specifically activated by mitogen-activated protein kinase kinase 5 (MAP2K5/MEK5). It is involved in the downstream signaling processes of various receptor molecules including receptor type kinases, and G protein-coupled receptors. In response to extracelluar signals, this kinase translocates to cell nucleus, where it regulates gene expression by phosphorylating, and activating different transcription factors. Four alternatively spliced transcript variants of this gene encoding two distinct isoforms have been reported.
      Gene Symbol
      • MAPK7
      • ERK5
      • BMK1
      • ERK-5
      • ERK4
      • PRKM7
      • Phosphorylation
      Purification MethodImmunoAffinity Purified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q13164 # MEK5 and ERK5 interact specifically with one another and not with MEK1/ERK1 or MEK2/ERK2 pathways.
      COFACTOR: Magnesium (By similarity).
      SIZE: 815 amino acids; 88637 Da
      TISSUE SPECIFICITY: Expressed in many adult tissues. Abundant in heart, placenta, lung, kidney and skeletal muscle. Not detectable in liver.
      DOMAIN:SwissProt: Q13164 The second proline-rich region may interact with actin targeting the kinase to a specific location in the cell. & The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
      PTM: Dually phosphorylated on Thr-218 and Tyr-220, which activates the enzyme (By similarity). Autophosphorylated in vitro on threonine and tyrosine residues when the C-terminal part of the kinase, which could have a regulatory role, is absent.
      SIMILARITY: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily. & Contains 1 protein kinase domain.
      Molecular Weight110-115kDa
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting/Peptide Inhibition Analysis in HeLa cell lysate.

      Western Blot/Peptide Inhibition Analysis: A 1:1,000 dilution of this antibody detected phospho-BMK1/Erk5 (Thr218/Tyr220) in HeLa cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain for 1 year at 2-8°C from date of shipment.
      Packaging Information
      Material Size200 µL
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-phospho-BMK1/Erk5 (Thr218, Tyr220) (rabbit immunoaffinity purified IgG) - 2135175 2135175
      Anti-phospho-BMK1/Erk5 (Thr218, Tyr220) - 2006533 2006533
      Anti-phospho-BMK1/Erk5 (Thr218, Tyr220) - JBC1370001 JBC1370001
      Anti-phospho-BMK1/Erk5 (Thr218, Tyr220) - JBC188183 JBC188183
      Anti-phospho-BMK1/Erk5 (Thr218/Tyr220) - 2391110 2391110
      Anti-phospho-BMK1/Erk5 (Thr218/Tyr220) - 2344693 2344693
      Anti-phospho-BMK1/Erk5 (Thr218/Tyr220) - 24621 24621
      Anti-phospho-BMK1/Erk5 (Thr218/Tyr220) - 28744 28744
      Anti-phospho-BMK1/Erk5 (Thr218/Tyr220) - Q2294572 Q2294572
      Anti-phospho-BMK1/Erk5 (Thr218/Tyr220) -2514056 2514056
      Anti-phospho-BMK1/Erk5 (Thr218/Tyr220) -2585808 2585808
      Anti-phospho-BMK1/Erk5 (Thr218/Tyr220) -2710224 2710224
      Anti-phospho-BMK1/Erk5 (Thr218/Tyr220) -2739294 2739294
      Anti-phospho-BMK1/Erk5 -2786036 2786036


      Reference overviewPub Med ID
      Activity assays for extracellular signal-regulated kinase 5.
      Kazuhiro Nakamura,Gary L Johnson
      Methods in molecular biology (Clifton, N.J.) 661 2010

      Show Abstract
      20811978 20811978
      Components of a new human protein kinase signal transduction pathway.
      Zhou, G, et al.
      J. Biol. Chem., 270: 12665-9 (1995) 1995

      Show Abstract
      7759517 7759517

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