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MABS748 | Anti-phospho-B-Raf (Ser729), clone EPR2207, Rabbit Monoclonal Antibody

100 µL  
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      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      R WB Rb Unpurified Monoclonal Antibody
      Catalogue NumberMABS748
      DescriptionAnti-phospho-B-Raf (Ser729), clone EPR2207, Rabbit Monoclonal Antibody
      Alternate Names
      • Serine/threonine-protein kinase B-raf
      Background InformationSerine/threonine-protein kinase B-raf, also known as Proto-oncogene B-Raf or p94 or v-Raf murine sarcoma viral oncogene homolog B1, and encoded by the gene name BRAF or BRAF1 or RAFB1, is a Serine/Threonine protein kinase member of the Raf family which includes Raf-1 and A-Raf. B-Raf is involved in the transduction of mitogenic signals from the cell membrane to the nucleus. Composed of three conserved region (CR1, CR2, CR3) B-Raf is expressed primarily in the brain and in the nervous system. It has been observed that MAPK is activated by B-Raf in response to nerve growth factor. More than 60% of malignant melanomas were found to contain a specific mutation, B-Raf (V599E) the product of which possesses constitutive kinase activity. Mutations in B-Raf have also been identified in lung cancer and non-Hodgkin lymphoma.
      Product Information
      PresentationRabbit monoclonal in buffer containing PBS with up to 0.1% sodium azide, 0.05% BSA, and 50% glycerol.
      ApplicationThis Anti-phospho-B-Raf (Ser729), clone EPR2207, Rabbit Monoclonal Antibody is validated for use in Western Blotting for the detection of phospho-B-Raf (Ser729).
      Key Applications
      • Western Blotting
      Application NotesProduced in collaboration with Epitomics™
      Biological Information
      ImmunogenSynthetic peptide corresponding to human phospho-B-Raf (Ser729).
      ConcentrationPlease refer to lot specific datasheet.
      Species Reactivity
      • Rat
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • BRAF
      • BRAF1
      • RAFB1
      Purification MethodUnpurified
      UniProt Number
      Molecular Weight~84 kDa observed. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in PC-12 untreated and PC-12 treated with Lambda Phosphatase cell lysates.

      Western Blotting Analysis: A 1:1,000-10,000 dilution of this antibody detected phospho-B-Raf (Ser729) in PC-12 untreated and PC-12 treated with Lambda Phosphatase cell lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information

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      Life Science Research > Antibodies and Assays > Primary Antibodies