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16-306 | Anti-phospho-Akt1/PKBα (Ser473) Antibody, clone 11E6, Alexa Fluor® 555

16-306
50 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      Ca, H, M, R FC, ICC, IF, WB M AlexaFluor®555 Monoclonal Antibody
      Description
      Catalogue Number16-306
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-phospho-Akt1/PKBα (Ser473) Antibody, clone 11E6, Alexa Fluor® 555
      Alternate Names
      • PKB
      • PKD-alpha
      • AKT1
      • Protein Kinase B
      References
      Product Information
      FormatAlexaFluor®555
      Applications
      ApplicationThis Anti-Akt1 Antibody, clone 11E6, Alexa Fluor 555 is validated for use in FC, IC, IF, WB for the detection of phospho-Akt1/PKBα (Ser473).
      Key Applications
      • Flow Cytometry
      • Immunocytochemistry
      • Immunofluorescence
      • Western Blotting
      Biological Information
      ImmunogenKLH conjugated synthetic peptide containing a pSer that corresponds to amino acid residues around phosphoserine 473.
      Clone11E6
      HostMouse
      SpecificityRecognizes phosphorylatedAkt1/PKBα at Ser473. This lot of antibody does not cross-react with Akt1/PKBá dephosphorylated at Ser473.
      IsotypeIgG1
      Species Reactivity
      • Canine
      • Human
      • Mouse
      • Rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Multiple alternatively spliced transcript variants have been found for this gene.
      Gene Symbol
      • AKT1
      • RAC
      • PRKBA
      • MGC99656
      • RAC-ALPHA
      • RAC-PK-alpha
      • C-AKT
      • PKB
      • AKT
      Modifications
      • Phosphorylation
      Purification MethodPurified from serum-free cell culture supernatant by subsequent thiophilic adsorption and size exclusion chromatography
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P31749 # General protein kinase capable of phosphorylating several known proteins. Phosphorylates TBC1D4. Signals downstream of phosphatidylinositol 3-kinase (PI(3)K) to mediate the effects of various growth factors such as platelet-derived growth factor (PDGF), epidermal growth factor (EGF), insulin and insulin-like growth factor I (IGF-I). Plays a role in glucose transport by mediating insulin-induced translocation of the GLUT4 glucose transporter to the cell surface. Mediates the antiapoptotic effects of IGF-I. Mediates insulin-stimulated protein synthesis, partly by playing a role in both insulin-induced phosphorylation of 4E-BP1 and in insulin-induced activation of p70 S6 kinase. Promotes glycogen synthesis by mediating the insulin-induced activation of glycogen synthase.
      SIZE: 480 amino acids; 55686 Da
      SUBUNIT: Interacts with CENTG1 isoform 2 (PIKE-A) in the presence of guanine nucleotides. The C-terminus interacts with CCDC88A/GRDN. Interacts with AKTIP.
      SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Note=Nucleus after activation by integrin-linked protein kinase 1 (ILK1).
      TISSUE SPECIFICITY: In all human cell types so far analyzed.
      DOMAIN: SwissProt: P31749 Binding of the PH domain to the phosphatidylinositol 3- kinase alpha (PI(3)K) results in its targeting to the plasma membrane.
      PTM: Phosphorylation on Thr-308, Ser-473 and Tyr-474 is required for full activity. Ser-473 phosphorylation by the Rictor-mTor complex favors Thr-308 phosphorylation by PDPK1. Ser-473 phosphorylation is enhanced by interaction with CENTG1 isoform 2 (PIKE-A).
      SIMILARITY: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. RAC subfamily. & Contains 1 AGC-kinase C-terminal domain. & Contains 1 PH domain. & Contains 1 protein kinase domain.
      Molecular Weight60 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by immunocytochemistry and flow cytometry.
      Usage Statement
      • Alexa Fluor® is a registered trademark of Molecular Probes, Inc.
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions1 year at 4°C from date of shipment
      Packaging Information
      Material Size50 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-phospho-Akt1/PKB (Ser473), clone 11E6, Alexa Fluor#174; 555 conjuga - DAM1598754 DAM1598754
      Anti-phospho-Akt1/PKBalpha; (Ser473), clone 11E6, Alexa Fluorreg; 555 conjugate - D6D027 D6D027
      Anti-phospho-Akt1/PKBα (Ser473), clone 11E6, Alexa Fluor® 555 conjugate -2592098 2592098

      References

      Reference overviewApplicationPub Med ID
      G-CSF activation of AKT is not sufficient to prolong neutrophil survival.
      Souza, LR; Silva, E; Calloway, E; Cabrera, C; McLemore, ML
      Journal of leukocyte biology 93 883-93 2013

      Show Abstract
      Fluorescence Activated Cell Sorting (FACS)23559492 23559492

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      Alternative Format

      Catalog Number Description  
      05-669 Anti-phospho-Akt1/PKBα (Ser473) Antibody, clone 11E6 Show Pricing & Availability
      16-305 Anti-phospho-Akt1/PKBα (Ser473) Antibody, clone 11E6, Alexa Fluor® 488 Show Pricing & Availability

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies