OP33 Anti-p53 (Ab-5) (Wild type) Mouse mAb (PAb1620)

OP33
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      B, H, M, Pm, R M Monoclonal Antibody

      Pricing & Availability

      Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
      OP33-100UG
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          Plastic ampoule 100 μg
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          OP33-20UG
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              Plastic ampoule 20 μg
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              Description
              OverviewRecognizes the ~53 kDa wild-type p53 protein in its native conformation in Hs27 cells and breast carcinoma tissue. Does not recognize mutant or denatured p53 protein.
              Catalogue NumberOP33
              Brand Family Calbiochem®
              References
              ReferencesEl-Deiry, W.S., et al. 1994. Cancer Res. 54, 1169.
              Greenblatt, M.S., et al. 1994. Cancer Res. 54, 4855.
              Barak, Y., et al. 1993. EMBO J. 12, 461.
              Kastan, M.B., et al. 1992. Cell 71, 587.
              Kuerbitz, S.J. 1992. Proc. Natl. Acad. Sci. USA 89, 7491.
              Lane, D.P. 1992. Nature 358, 15.
              Kastan, M.B., et al. 1991. Cancer Res. 51, 6304.
              Ball, R.K., et al. 1984. EMBO J. 3, 1485.
              Product Information
              FormLiquid
              FormulationIn 0.05 M sodium phosphate buffer, 0.2% gelatin.
              Positive controlHs27 cells or breast carcinoma tissue
              Preservative≤0.1% sodium azide
              Applications
              Application ReferencesImmunoprecipitation Lu, X., et al. 1992. Cell 70, 153. Frozen Sections Kramata, P., et al. 2005. Cancer Res. 65, 3577. Cordon-Cardo, C., et al. 1991. Cancer Res. 54, 794.
              Key Applications Not Western Blot
              Paraffin Sections
              Frozen Sections
              Immunofluorescence
              Immunoprecipitation
              Application NotesFrozen Sections (see applications references)
              Immunoblotting (not recommended)
              Immunofluorescence (1-20 μg/ml)
              Immunoprecipitation (1 μg per sample)
              Paraffin Sections (5 μg/ml, heat pre-treatment required)
              Application CommentsWild-type p53 has a short half-life and is present in low amounts in cells. Increasing the amount of sample to be immunoprecipitated and applied to the gel may help for visualization. Short incubation times with 35S-Met(≤ 1 hr) will help reduce background. p53 (Ab-5) may also be used to visualize p53 in cytospins or cultured cells using standard horseradish peroxidase or immunofluorescence detection techniques. p53 (Ab-5) preferentially immunoprecipitates wild-type p53; it should not precipitate mutant or denatured p53. Antibody should be titrated for optimal results in individual systems.
              Biological Information
              ImmunogenvLM mouse tumor cells
              ImmunogenMouse
              ClonePAb1620
              HostMouse
              IsotypeIgG2a
              Species Reactivity
              • Bovine
              • Human
              • Mouse
              • Primate
              • Rat
              Antibody TypeMonoclonal Antibody
              Concentration Label Please refer to vial label for lot-specific concentration
              Physicochemical Information
              Dimensions
              Materials Information
              Toxicological Information
              Safety Information according to GHS
              Safety Information
              Product Usage Statements
              Storage and Shipping Information
              Ship Code Blue Ice Only
              Toxicity Standard Handling
              Storage +2°C to +8°C
              Do not freeze Yes
              Packaging Information
              Transport Information
              Supplemental Information
              Specifications

              Documentation

              Anti-p53 (Ab-5) (Wild type) Mouse mAb (PAb1620) SDS

              Title

              Safety Data Sheet (SDS) 

              Anti-p53 (Ab-5) (Wild type) Mouse mAb (PAb1620) Certificates of Analysis

              TitleLot Number
              OP33

              References

              Reference overview
              El-Deiry, W.S., et al. 1994. Cancer Res. 54, 1169.
              Greenblatt, M.S., et al. 1994. Cancer Res. 54, 4855.
              Barak, Y., et al. 1993. EMBO J. 12, 461.
              Kastan, M.B., et al. 1992. Cell 71, 587.
              Kuerbitz, S.J. 1992. Proc. Natl. Acad. Sci. USA 89, 7491.
              Lane, D.P. 1992. Nature 358, 15.
              Kastan, M.B., et al. 1991. Cancer Res. 51, 6304.
              Ball, R.K., et al. 1984. EMBO J. 3, 1485.

              Brochure

              Title
              p53 Pathway Poster ( 386 KB )

              Citations

              Title
            • Pavel Kramata, et al. (2005) Patches of Mutant p53-Immunoreactive Epidermal Cells Induced by Chronic UVB Irradiation Harbor the Same p53 Mutations as Squamous Cell Carcinomas in the Skin in Hairless SKH-1 Mice. Cancer Research 65, 3577-3585.
            • Data Sheet

              Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

              Revision02-October-2007 RFH
              ApplicationFrozen Sections (see applications references)
              Immunoblotting (not recommended)
              Immunofluorescence (1-20 μg/ml)
              Immunoprecipitation (1 μg per sample)
              Paraffin Sections (5 μg/ml, heat pre-treatment required)
              DescriptionPurified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with SP2/0 Ag14 myeloma cells. Recognizes the ~53 kDa wild-type p53 protein.
              BackgroundThe human p53 tumor suppressor gene encodes a 393 amino acid phospho-protein that exhibits sequence-specific DNA binding and directly interacts with various cellular and viral proteins. p53 is the most commonly mutated gene in human cancer, with the majority of the mutations being amino acid substitutions. The normal function of p53 is to effect cell cycle arrest at the G1 and G2 checkpoints in response to DNA damage. This checkpoint function is executed by accumulation of p53 followed by induction of the GADD45, WAF1 and MDM2 genes. The current model of p53 function postulates that p53 senses DNA damage and arrests the cell cycle in either the G1 or G2 phases to allow DNA repair to take place. If repair is not successful, p53 initiates programmed cell death, thus preventing the propagation of genetic defects to successive generations of cells.
              HostMouse
              Immunogen speciesMouse
              ImmunogenvLM mouse tumor cells
              ClonePAb1620
              IsotypeIgG2a
              Speciesbovine, human, mouse, primate, rat
              Positive controlHs27 cells or breast carcinoma tissue
              FormLiquid
              FormulationIn 0.05 M sodium phosphate buffer, 0.2% gelatin.
              Concentration Label Please refer to vial label for lot-specific concentration
              Preservative≤0.1% sodium azide
              CommentsWild-type p53 has a short half-life and is present in low amounts in cells. Increasing the amount of sample to be immunoprecipitated and applied to the gel may help for visualization. Short incubation times with 35S-Met(≤ 1 hr) will help reduce background. p53 (Ab-5) may also be used to visualize p53 in cytospins or cultured cells using standard horseradish peroxidase or immunofluorescence detection techniques. p53 (Ab-5) preferentially immunoprecipitates wild-type p53; it should not precipitate mutant or denatured p53. Antibody should be titrated for optimal results in individual systems.
              Storage +2°C to +8°C
              Do Not Freeze Yes
              Toxicity Standard Handling
              ReferencesEl-Deiry, W.S., et al. 1994. Cancer Res. 54, 1169.
              Greenblatt, M.S., et al. 1994. Cancer Res. 54, 4855.
              Barak, Y., et al. 1993. EMBO J. 12, 461.
              Kastan, M.B., et al. 1992. Cell 71, 587.
              Kuerbitz, S.J. 1992. Proc. Natl. Acad. Sci. USA 89, 7491.
              Lane, D.P. 1992. Nature 358, 15.
              Kastan, M.B., et al. 1991. Cancer Res. 51, 6304.
              Ball, R.K., et al. 1984. EMBO J. 3, 1485.
              Citation
            • Pavel Kramata, et al. (2005) Patches of Mutant p53-Immunoreactive Epidermal Cells Induced by Chronic UVB Irradiation Harbor the Same p53 Mutations as Squamous Cell Carcinomas in the Skin in Hairless SKH-1 Mice. Cancer Research 65, 3577-3585.
            • Application referencesImmunoprecipitation Lu, X., et al. 1992. Cell 70, 153. Frozen Sections Kramata, P., et al. 2005. Cancer Res. 65, 3577. Cordon-Cardo, C., et al. 1991. Cancer Res. 54, 794.

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              Categories

              Life Science Research > Antibodies and Assays > Primary Antibodies