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OP140 | Anti-p53 (Ab-12) (Pantropic) Mouse mAb (DO-7)

OP140
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      B, H, Mk M Monoclonal Antibody

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      OP140-100UG
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      Stocked 
      Discontinued
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      Available
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          Glass bottle 100 μg
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          Description
          OverviewRecognizes the ~53 kDa wild-type p53 and mutant p53 protein in SKBR-3 and MDA-231 cells and in colon carcinoma tissue.
          Catalogue NumberOP140
          Brand Family Calbiochem®
          Application Data
          Detection of human p53 by staining paraffin sections. Sample: Human colon carcinoma fixed in formalin and paraffin-embedded. Primary antibody: Anti-p53 (Ab-12) (Pantropic) Mouse mAb (DO-7) (Cat. No. OP140) (1 µg/ml) Detection: DAB.

          Detection of human p53 by immunoblotting. Sample: Human p53. Primary antibody: Anti-p53 (Ab-12) (Pantropic) Mouse mAb (DO-7) (Cat. No. OP140) (1 µg/ml). Detection: chemiluminescence.
          References
          ReferencesGreenblatt, M.S., et al. 1994. Cancer Res. 54, 4855.
          Lane, D.P. 1992. Nature 358, 15.
          Product Information
          FormLiquid
          FormulationIn 10 mM PBS, pH 7.4.
          Positive controlSKBR-3 or MDA-231 cells or colon carcinoma tissue
          PreservativeNone
          Applications
          Key Applications Frozen Sections
          Immunoblotting (Western Blotting)
          Immunofluorescence
          Immunoprecipitation
          Paraffin Sections
          Application NotesFrozen Sections (1-2 µg/ml)
          Immunoblotting (1-2 µg/ml)
          Immunofluorescence (0.5 µg/ml)
          Immunoprecipitation (2 µg/mg lysate)
          Paraffin Sections (1-2 µg/ml)
          Application CommentsExhibits nuclear staining in tumor cells. Antibody should be titrated for optimal results in individual systems.
          Biological Information
          Immunogenrecombinant, human p53 expressed in E. coli
          ImmunogenHuman
          Epitopewithin amino acids 37-45 of p53
          CloneDO-7
          HostMouse
          IsotypeIgG2b
          Species Reactivity
          • Bovine
          • Human
          • Monkey
          Antibody TypeMonoclonal Antibody
          Concentration Label Please refer to vial label for lot-specific concentration
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Dry Ice Only
          Toxicity Standard Handling
          Storage -20°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          OP140

          References

          Reference overview
          Greenblatt, M.S., et al. 1994. Cancer Res. 54, 4855.
          Lane, D.P. 1992. Nature 358, 15.

          Brochure

          Title
          DNA Repair Pathway Poster
          p53 Pathway Poster ( 386 KB )
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision02-March-2009 JSW
          ApplicationFrozen Sections (1-2 µg/ml)
          Immunoblotting (1-2 µg/ml)
          Immunofluorescence (0.5 µg/ml)
          Immunoprecipitation (2 µg/mg lysate)
          Paraffin Sections (1-2 µg/ml)
          Application Data
          Detection of human p53 by staining paraffin sections. Sample: Human colon carcinoma fixed in formalin and paraffin-embedded. Primary antibody: Anti-p53 (Ab-12) (Pantropic) Mouse mAb (DO-7) (Cat. No. OP140) (1 µg/ml) Detection: DAB.

          Detection of human p53 by immunoblotting. Sample: Human p53. Primary antibody: Anti-p53 (Ab-12) (Pantropic) Mouse mAb (DO-7) (Cat. No. OP140) (1 µg/ml). Detection: chemiluminescence.
          DescriptionPurified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen. Recognizes the ~53 kDa wild-type and mutant forms of p53.
          BackgroundThe human p53 tumor suppressor gene encodes a 393 amino acid phosphoprotein that exhibits sequence-specific DNA binding and directly interacts with various cellular and viral proteins. p53 is the most commonly mutated gene in human cancer, with the majority of the mutations being amino acid substitutions. The normal function of p53 is to effect cell cycle arrest at the G1 and G2 checkpoints in response to DNA damage. This checkpoint function is executed by accumulation of p53 followed by induction of the GADD45, WAF1, and MDM2 genes. The current model of p53 function postulates that p53 senses DNA damage and arrests the cell cycle in either the G1 or G2 phases to allow DNA repair to take place. If repair is not successful, p53 initiates programmed cell death, thus preventing the propagation of genetic defects to successive generations of cells. Cat. No. OP140 recognizes a 53 kDa phosphoprotein. The antibody binds to both mutant and wild-type p53.
          HostMouse
          Immunogen speciesHuman
          Immunogenrecombinant, human p53 expressed in E. coli
          Epitopewithin amino acids 37-45 of p53
          CloneDO-7
          IsotypeIgG2b
          Speciesbovine, human, monkey
          Positive controlSKBR-3 or MDA-231 cells or colon carcinoma tissue
          FormLiquid
          FormulationIn 10 mM PBS, pH 7.4.
          Concentration Label Please refer to vial label for lot-specific concentration
          PreservativeNone
          CommentsExhibits nuclear staining in tumor cells. Antibody should be titrated for optimal results in individual systems.
          Storage -20°C
          Avoid freeze/thaw
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Toxicity Standard Handling
          ReferencesGreenblatt, M.S., et al. 1994. Cancer Res. 54, 4855.
          Lane, D.P. 1992. Nature 358, 15.