506123 | Anti-p38 MAP Kinase (341-360) Rabbit pAb

506123
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      H, M, R Rb Polyclonal Antibody

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      506123-200UL
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      Stocked 
      Discontinued
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      Available
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          Plastic ampoule 200 ul
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          Description
          OverviewRecognizes the ~38 kDa p38 MAPK protein.
        • Antibody Target Gene Symbol: MAPK14
        • Target Synonym: CRK1, CSBP, CSBP1, CSBP2, CSPB1, EXIP, Hog, MAPK p38, MGC102436, MGC105413, MXI2, P38, P38 KINASE, P38 Map Kinase, p38 Mapk alpha, P38-ALPHA, p38-RK, p38/Hog1, p38/Mpk2, P38/RK, p38a, p38Hog, p38MAPK, PRKM14, PRKM15, RK, SAPK2A
        • Entrez Gene Name: mitogen-activated protein kinase 14
        • Hu Entrez ID: 1432
        • Mu Entrez ID: 26416
        • Rat Entrez ID: 81649
        • Catalogue Number506123
          Brand Family Calbiochem®
          Application Data
          Detection of p38 MAP kinase by immunoblotting. Samples: C6 cells (lanes 1 and 2) treated (lane 1) or untreated (lane 2) with anisomycin and NIH/3T3 cells (lanes 3 and 4) treated (lane 3) or untreated (lane 4) with UV. Primary antibody: Anti-p38 MAP Kinase (341-360) Rabbit pAb (Cat. No. 506123) (1:1000). Detection: chemiluminescence.
          References
          ReferencesRaingeaud, J., et al. 1995. J. Biol. Chem. 270, 7420.
          Zervos, A.S., et al. 1995. Proc. Natl. Acad. Sci. USA 92, 10531.
          Han, J., et al. 1994. Science 265, 808.
          Lee, J.C., et al. 1994. Nature 372, 739.
          Rouse, J., et al. 1994. Cell 78, 1027.
          Product Information
          FormLiquid
          FormulationIn 150 mM NaCl, 10 mM HEPES, 50% glycerol, 0.01% BSA, pH 7.5.
          PreservativeNone
          Applications
          Key Applications Flow Cytometry
          Immunoblotting (Western Blotting)
          Paraffin Sections
          Application NotesFlow Cytometry (1:25)
          Immunoblotting (1:1000)
          Paraffin Sections (1:50, heat pretreatment required, see comments)
          Application CommentsPretreat paraffin sections by heating tissue in 10 mM citrate buffer, pH 6.0 for 1 min at high power followed by 9 min at medium power; keep the slides fully immersed and maintain the temperature at or just below boiling; cool the slides for 20 min at room temperature prior to staining. Variables associated with assay conditions will dictate the proper working dilution.

          Recommended Protocol for Immunoblotting

          Solutions and Reagents
          • Transfer Buffer: 25 mM Tris base, 0.2 M glycine, 20% methanol, pH 8.5.
          • SDS Sample Buffer: 62.5 mM Tris-HCl, pH 6.8, 2% SDS, 10% glycerol, 50 mM DTT, 0.1% bromophenol blue.
          • 10X TBS (Tris-buffered saline): To prepare 1 liter, 24.2 g Tris base, 80 g NaCl, adjust pH to 7.6 with HCl. Dilute 1:10 for use.
          • Blocking Buffer: 1X TBS, 0.1% Tween®-20 detergent with 5% non-fat dry milk.
          • Primary Antibody Dilution Buffer: 1X TBS, 0.1% Tween®-20 detergent with 5% BSA
          • Wash Buffer (TBST): 1X TBS, 0.1% Tween®-20 detergent

          Blotting Membrane
          Nitrocellulose or PVDF membranes may be used.

          Protein Blotting
          1. Lyse cells by adding 100 ml SDS Sample Buffer and immediately scrape the cells off the plate and transfer the extract to a microfuge tube. Keep on ice.
          2. Sonicate for 2 s to shear DNA and reduce sample viscosity.
          3. Heat sample to 95-100°C for 5 min. Cool on ice.
          4. Microcentrifuge for 5 min.
          5. Load 20 ml onto SDS-PAGE gel (10 cm x 10 cm).
          6. Electrotransfer to nitrocellulose membrane.

          As controls, we recommend using 15 ml of phosphorylated and nonphosphorylated C-6 glioma cell extracts.

          Membrane Blocking, Gel and Antibody Incubations
          1. After transfer, wash membrane with 25 ml TBS for 5 min at room temperature.
          2. Incubate membrane in 25 ml of Blocking Buffer for 1-3 h at room temperature or overnight at 4°C.
          3. Wash 3 times for 5 min each with 15 ml TBST.
          4. Incubate membrane and primary antibody (at the appropriate dilution) in 10 ml Primary Antibody Dilution Buffer with gentle agitation overnight at 4°C.
          5. Wash 3 times for 5 min each with 15 ml TBST.
          6. Incubate membrane with conjugated secondary antibody at the appropriate dilution in 10 ml Blocking Buffer with gentle agitation for 1 h at room temperature.
          7. Wash membrane as in step 5.

          Detection of Proteins
          Chemiluminescence.
          Biological Information
          Immunogena synthetic peptide (TYDEYISFVPPPLDQEEMES) corresponding to amino acids 341-360 of human p38 MAP kinase
          ImmunogenHuman
          HostRabbit
          IsotypeIgG
          Species Reactivity
          • Human
          • Mouse
          • Rat
          Antibody TypePolyclonal Antibody
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Blue Ice Only
          Toxicity Standard Handling
          Storage -20°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          506123

          References

          Reference overview
          Raingeaud, J., et al. 1995. J. Biol. Chem. 270, 7420.
          Zervos, A.S., et al. 1995. Proc. Natl. Acad. Sci. USA 92, 10531.
          Han, J., et al. 1994. Science 265, 808.
          Lee, J.C., et al. 1994. Nature 372, 739.
          Rouse, J., et al. 1994. Cell 78, 1027.

          Brochure

          Title
          Antibody Sourcebook!" Second Edition
          DNA Repair Pathway Poster
          MAP Kinases Technical Bulletin
          MAPK Pathway Poster ( 750 KB )
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision17-July-2007 RFH
          ApplicationFlow Cytometry (1:25)
          Immunoblotting (1:1000)
          Paraffin Sections (1:50, heat pretreatment required, see comments)
          Application Data
          Detection of p38 MAP kinase by immunoblotting. Samples: C6 cells (lanes 1 and 2) treated (lane 1) or untreated (lane 2) with anisomycin and NIH/3T3 cells (lanes 3 and 4) treated (lane 3) or untreated (lane 4) with UV. Primary antibody: Anti-p38 MAP Kinase (341-360) Rabbit pAb (Cat. No. 506123) (1:1000). Detection: chemiluminescence.
          DescriptionProtein A and immunoaffinity purified rabbit polyclonal antibody. Recognizes the ~38 kDa p38 MAPK protein.
          Backgroundp38 MAP kinase (MAPK), also called RK and CSBP, is the mammalian homologue of the yeast HOG kinase and participates in a cascade controlling cellular responses to cytokines and stress. Like the SAPK/JNK pathway, p38 MAPK is activated by a variety of cellular stresses including osmotic shock, inflammatory cytokines, lipopolysaccharides (LPS), UV light and growth factors. Both MKK3 and SEK phosphorylate p38 on tyrosine and threonine at the sequence T*GY* resulting in p38 activation. Activated p38 MAPK has been shown to phosphorylate and activate MAPKAP kinase-2 and to phosphorylate the transcription factors ATF-2 and Max.
          HostRabbit
          Immunogen speciesHuman
          Immunogena synthetic peptide (TYDEYISFVPPPLDQEEMES) corresponding to amino acids 341-360 of human p38 MAP kinase
          IsotypeIgG
          Specieshuman, mouse, rat
          FormLiquid
          FormulationIn 150 mM NaCl, 10 mM HEPES, 50% glycerol, 0.01% BSA, pH 7.5.
          PreservativeNone
          CommentsPretreat paraffin sections by heating tissue in 10 mM citrate buffer, pH 6.0 for 1 min at high power followed by 9 min at medium power; keep the slides fully immersed and maintain the temperature at or just below boiling; cool the slides for 20 min at room temperature prior to staining. Variables associated with assay conditions will dictate the proper working dilution.

          Recommended Protocol for Immunoblotting

          Solutions and Reagents
          • Transfer Buffer: 25 mM Tris base, 0.2 M glycine, 20% methanol, pH 8.5.
          • SDS Sample Buffer: 62.5 mM Tris-HCl, pH 6.8, 2% SDS, 10% glycerol, 50 mM DTT, 0.1% bromophenol blue.
          • 10X TBS (Tris-buffered saline): To prepare 1 liter, 24.2 g Tris base, 80 g NaCl, adjust pH to 7.6 with HCl. Dilute 1:10 for use.
          • Blocking Buffer: 1X TBS, 0.1% Tween®-20 detergent with 5% non-fat dry milk.
          • Primary Antibody Dilution Buffer: 1X TBS, 0.1% Tween®-20 detergent with 5% BSA
          • Wash Buffer (TBST): 1X TBS, 0.1% Tween®-20 detergent

          Blotting Membrane
          Nitrocellulose or PVDF membranes may be used.

          Protein Blotting
          1. Lyse cells by adding 100 ml SDS Sample Buffer and immediately scrape the cells off the plate and transfer the extract to a microfuge tube. Keep on ice.
          2. Sonicate for 2 s to shear DNA and reduce sample viscosity.
          3. Heat sample to 95-100°C for 5 min. Cool on ice.
          4. Microcentrifuge for 5 min.
          5. Load 20 ml onto SDS-PAGE gel (10 cm x 10 cm).
          6. Electrotransfer to nitrocellulose membrane.

          As controls, we recommend using 15 ml of phosphorylated and nonphosphorylated C-6 glioma cell extracts.

          Membrane Blocking, Gel and Antibody Incubations
          1. After transfer, wash membrane with 25 ml TBS for 5 min at room temperature.
          2. Incubate membrane in 25 ml of Blocking Buffer for 1-3 h at room temperature or overnight at 4°C.
          3. Wash 3 times for 5 min each with 15 ml TBST.
          4. Incubate membrane and primary antibody (at the appropriate dilution) in 10 ml Primary Antibody Dilution Buffer with gentle agitation overnight at 4°C.
          5. Wash 3 times for 5 min each with 15 ml TBST.
          6. Incubate membrane with conjugated secondary antibody at the appropriate dilution in 10 ml Blocking Buffer with gentle agitation for 1 h at room temperature.
          7. Wash membrane as in step 5.

          Detection of Proteins
          Chemiluminescence.
          Storage -20°C
          Avoid freeze/thaw
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Toxicity Standard Handling
          ReferencesRaingeaud, J., et al. 1995. J. Biol. Chem. 270, 7420.
          Zervos, A.S., et al. 1995. Proc. Natl. Acad. Sci. USA 92, 10531.
          Han, J., et al. 1994. Science 265, 808.
          Lee, J.C., et al. 1994. Nature 372, 739.
          Rouse, J., et al. 1994. Cell 78, 1027.