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07-571-I | Anti-nNOS/NOS I Antibody

07-571-I
200 µL  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      R, M WB, IH(P) Rb Affinity Purified Polyclonal Antibody
      Description
      Catalogue Number07-571-I
      Replaces07-571
      DescriptionAnti-nNOS/NOS I Antibody
      Alternate Names
      • Nitric oxide synthase, brain
      • bNOS
      • Constitutive NOS
      • NC-NOS
      • NOS type I
      • Neuronal NOS
      • N-NOS
      • nNOS
      • Peptidyl-cysteine S-nitrosylase NOS1
      Background InformationNitric oxide (NO) is an inorganic, gaseous free radical that carries a variety of messages between cells. nNOS/NOS I, also known as NC-NOS, or NOS type I, or Neuronal NOS, or Peptidyl-cysteine S-nitrosylase NOS1 and bNOS produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In the brain and peripheral nervous system, NO displays many properties of a neurotransmitter. NOS I has been suggested to have nitrosylase activity and to mediate cysteine S-nitrosylation of cytoplasmic target proteins such SRR. Vasorelaxation, neurotransmission and cytotoxicity can all be potentiated through cellular response to NO. NO production is mediated by members of the nitric oxide synthase (NOS) family. NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO. Two constitutive isoforms, brain or neuronal NOS (b or nNOS, type I) & endothelial cell NOS (eNOS, type III), and one inducible isoform (iNOS, type II), have been cloned. Cytokines such as interferon-gamma (IFN), tumor necrosis factor (TNF), interleukin-1 and -2, and lipopolysaccarides (LPS) cause an increase in iNOS mRNA, protein, and activity levels. Protein kinase C-stimulating agents exhibit the same effect on iNOS activity. Human iNOS is regulated by calcium/calmodulin (in contrast with mouse NOS2).
      References
      Product Information
      FormatAffinity Purified
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-nNOS/NOS I Antibody is an antibody against nNOS/NOS I for use in western blotting & IHC (Paraffin).
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      Application NotesImmunohistochemistry Analysis: A 1:2,000 dilution from a representative lot detected nNOS/NOS I in rat cerebellum and cerebral cortex tissues, and mouse hippocampus and thalamus tissues.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to the near N-terminus of rat nNOS/NOS I.
      EpitopeNear N-terminus
      Concentration0.5 mg/mL
      HostRabbit
      Species Reactivity
      • Rat
      • Mouse
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Nos1
      • Bnos
      Purification MethodAffinity Purfied
      UniProt Number
      Molecular Weight~165 kDa observed. Uncharacterized band(s) may be observed in some cell lysates.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in rat brain microsomal preparation.

      Western Blotting Analysis: A 1:500 dilution of this antibody detected nNOS/NOS I in 10 µg of rat brain microsomal preparation.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size200 µL
      Transport Information
      Supplemental Information
      Specifications