07-436 Anti-monomethyl-Histone H3 (Lys4) Antibody

200 µg  
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      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, Vrt ICC, IF, PIA, WB, ChIP-seq, DB Rb Purified Polyclonal Antibody
      Catalogue Number07-436
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-monomethyl-Histone H3 (Lys4) Antibody
      Alternate Names
      • H3K4me1
      • Histone H3 (mono methyl K4)
      • H3 histone family, member T
      • histone 3
      • H3
      • histone cluster 3
      • H3
      Background InformationHistone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.
      Product Information
      • HeLa cell extract
      PresentationPurified rabbit IgG in buffer containing 0.1M Tris-Glycine, 0.15M NaCl, 0.05 Sodium Azide, pH 7.4.
      ApplicationAnti-monomethyl-Histone H3 (Lys4) Antibody is a rabbit polyclonal antibody for detection of monomethyl-Histone H3 (Lys4) also known as H3K4me1, Histone H3 (mono methyl K4) & has been validated in ICC, IF, PIA, WB, ChIP-seq, DB.
      Key Applications
      • Immunocytochemistry
      • Immunofluorescence
      • Peptide Inhibition Assay
      • Western Blotting
      • ChIP-seq
      • Dot Blot
      Application NotesImmunocytochemistry:
      Reported by an independent laboratory.

      Peptide Inhibition:
      Specificity was confirmed by the ability of 1 μM of the immunizing peptide to completely abolish detection of histone H3 in immunoblot analysis of HeLa acid extracts. (see figure, lane 3). No competition was observed with peptides containing dimethyl-lysine 4, trimethyl-lysine 4, mono-methyllysine 4 or monomethyl-lysine 9 or 27.
      Biological Information
      ImmunogenKLH-conjugated, synthetic peptide containing the sequence ART[meK]…, in which meK corresponds to monomethyl-lysine at residue 4 of human histone H3.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      SpecificityRecognizes monomethyl-histone H3 (Lys4), Mr 17kDa.
      Species Reactivity
      • Human
      • Vertebrates
      Species Reactivity NoteHuman. Broad species to cross-reactivity is expected.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryHistones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
      Gene Symbol
      • HIST3H3
      • H3FT
      • H3/t
      • H3t
      • MGC126888
      • H3/g
      • H3.4
      • H3T
      • MGC126886
      • Methylation
      Purification MethodProtein A Purfied
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q16695 # Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
      SIZE: 136 amino acids; 15508 Da
      SUBUNIT: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.
      TISSUE SPECIFICITY: Expressed in testicular cells.
      DEVELOPMENTAL STAGE: Expressed during S phase, then expression strongly decreases as cell division slows down during the process of differentiation.
      PTM: Acetylation is generally linked to gene activation. Acetylation on Lys-10 impairs methylation at Arg-9. Acetylation on Lys-19 and Lys-24 favors methylation at Arg-18 (By similarity). & Citrullination at Arg-9 and/or Arg-18 by PADI4 impairs methylation and represses transcription (By similarity). & Asymmetric dimethylation at Arg-18 by CARM1 is linked to gene activation. Symmetric dimethylation at Arg-9 by PRMT5 is linked to gene repression (By similarity). & Methylation at Lys-5, Lys-37 and Lys-80 are linked to gene activation. Methylation at Lys-5 facilitates subsequent acetylation of H3 and H4. Methylation at Lys-80 is associated with DNA double-strand break (DSB) responses and is a specific target for TP53BP1. Methylation at Lys-10 and Lys-28 are linked to gene repression. Methylation at Lys-10 is a specific target for HP1 proteins (CBX1, CBX3 and CBX5) and prevents subsequent phosphorylation at Ser-11 and acetylation of H3 and H4. Methylation at Lys-5 and Lys-80 require preliminary monoubiquitination of H2B at 'Lys-120'. Methylation at Lys-10 and Lys-28 are enriched in inactive X chromosome chromatin (By similarity). & Phosphorylated at Thr-4 by GSG2/haspin during prophase and dephosphorylated during anaphase. At centromeres, specifically phosphorylated at Thr-12 from prophase to early anaphase. Phosphorylated at Ser-11 during the whole mitosis. Phosphorylation at Ser-11, which is linked to gene activation, prevents methylation at Lys-10 but facilitates acetylation of H3 and H4. Phosphorylated at Ser-29 by MLTK isoform 1, RPS6KA5 or AURKB during mitosis or upon ultraviolet B irradiation (By similarity). & Phosphorylation at 'Ser-11' is crucial for chromosome condensation and cell-cycle progression during mitosis and meiosis. In addition phosphorylation at 'Ser-11' is important during interphase because it enables the transcription of genes following external stimulation, like stress or growth factors. Phosphorylation at 'Ser-11' is also an essential regulatory mechanism for neoplastic cell transformation. Phosphorylation at 'Ser-11' by AURKB/Aurora-B mediates the dissociation of HP1 proteins (CBX1, CBX3 and CBX5) from heterochromatin. & Ubiquitinated (By similarity).
      SIMILARITY: SwissProt: Q16695 ## Belongs to the histone H3 family.
      Molecular Weight17 kDa
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by Western Blot in acid extracted proteins from HeLa cells.

      Western Blot Analysis:
      0.5-2 μg/mL of this lot detected methylated histone H3 in acid extracted proteins from HeLa cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2 to 8°C from date of receipt.
      Packaging Information
      Material Size200 µg
      Transport Information
      Supplemental Information


      Anti-monomethyl-Histone H3 (Lys4) Antibody SDS


      Safety Data Sheet (SDS) 

      Anti-monomethyl-Histone H3 (Lys4) Antibody Certificates of Analysis

      TitleLot Number
      Anti-monomethyl-Histone H3 (Lys4)2474976
      Anti-monomethyl-Histone H3 (Lys4) (rabbit polyclonal IgG) - DAM1400139DAM1400139
      Anti-monomethyl-Histone H3 (Lys4) - 21494912149491
      Anti-monomethyl-Histone H3 (Lys4) - 23435812343581
      Anti-monomethyl-Histone H3 (Lys4) - 23835322383532
      Anti-monomethyl-Histone H3 (Lys4) - 23989072398907
      Anti-monomethyl-Histone H3 (Lys4) - 19669351966935
      Anti-monomethyl-Histone H3 (Lys4) - 20195172019517
      Anti-monomethyl-Histone H3 (Lys4) - 20405312040531
      Anti-monomethyl-Histone H3 (Lys4) - 22838412283841


      Reference overviewApplicationSpeciesPub Med ID
      A comprehensive epigenome map of Plasmodium falciparum reveals unique mechanisms of transcriptional regulation and identifies H3K36me2 as a global mark of gene suppression.
      Karmodiya, K; Pradhan, SJ; Joshi, B; Jangid, R; Reddy, PC; Galande, S
      Epigenetics & chromatin  8  32  2015

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      26388940 26388940
      Intracellular α-ketoglutarate maintains the pluripotency of embryonic stem cells.
      Carey, BW; Finley, LW; Cross, JR; Allis, CD; Thompson, CB
      Nature  518  413-6  2015

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      ROW1 maintains quiescent centre identity by confining WOX5 expression to specific cells.
      Zhang, Y; Jiao, Y; Liu, Z; Zhu, YX
      Nature communications  6  6003  2015

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      Integrative genomic analysis reveals widespread enhancer regulation by p53 in response to DNA damage.
      Younger, ST; Kenzelmann-Broz, D; Jung, H; Attardi, LD; Rinn, JL
      Nucleic acids research  43  4447-62  2015

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      25883152 25883152
      Embryonic MicroRNA-369 Controls Metabolic Splicing Factors and Urges Cellular Reprograming.
      Konno, M; Koseki, J; Kawamoto, K; Nishida, N; Matsui, H; Dewi, DL; Ozaki, M; Noguchi, Y; Mimori, K; Gotoh, N; Tanuma, N; Shima, H; Doki, Y; Mori, M; Ishii, H
      PloS one  10  e0132789  2015

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      26176628 26176628
      Analysis of Histones H3 and H4 Reveals Novel and Conserved Post-Translational Modifications in Sugarcane.
      Moraes, I; Yuan, ZF; Liu, S; Souza, GM; Garcia, BA; Casas-Mollano, JA
      PloS one  10  e0134586  2015

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      26226299 26226299
      Feedback control of Set1 protein levels is important for proper H3K4 methylation patterns.
      Soares, LM; Radman-Livaja, M; Lin, SG; Rando, OJ; Buratowski, S
      Cell reports  6  961-72  2014

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      24613354 24613354
      Transcriptional repression is epigenetically marked by H3K9 methylation during SV40 replication.
      Kallestad, L; Christensen, K; Woods, E; Milavetz, B
      Clinical epigenetics  6  21  2014

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      25395994 25395994
      The rice enhancer of zeste [E(z)] genes SDG711 and SDG718 are respectively involved in long day and short day signaling to mediate the accurate photoperiod control of flowering time.
      Liu, X; Zhou, C; Zhao, Y; Zhou, S; Wang, W; Zhou, DX
      Frontiers in plant science  5  591  2014

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      Western Blotting25400654 25400654
      Epigenetic modifications are associated with inter-species gene expression variation in primates.
      Zhou, X; Cain, CE; Myrthil, M; Lewellen, N; Michelini, K; Davenport, ER; Stephens, M; Pritchard, JK; Gilad, Y
      Genome biology  15  547  2014

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      25468404 25468404

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