07-548 Anti-monomethyl-Histone H3 (Lys36) Antibody

07-548
100 µL  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      Vrt DB, IF, WB, Mplex Rb Serum Polyclonal Antibody
      Description
      Catalogue Number07-548
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-monomethyl-Histone H3 (Lys36) Antibody
      Alternate Names
      • H3K36me1
      • Histone H3 (mono methyl K36)
      References
      Product Information
      FormatSerum
      Presentation0.05% sodium azide and 30% glycerol
      Applications
      ApplicationUse Anti-monomethyl-Histone H3 (Lys36) Antibody (Rabbit Polyclonal Antibody) validated in DB, IF, WB, Mplex to detect monomethyl-Histone H3 (Lys36) also known as H3K36me1, Histone H3 (monomethyl K36).
      Key Applications
      • Dot Blot
      • Immunofluorescence
      • Western Blotting
      • Multiplexing
      Biological Information
      ImmunogenPeptide containing the sequence GVmeKKP corresponding to monomethyl Lysine 36 of human histone H3 .
      HostRabbit
      Specificitymonomethyl-Histone H3 (Lys36)
      IsotypeIgG
      Species Reactivity
      • Vertebrates
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryHistones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene contains introns and its mRNA is polyadenylated, unlike most histone genes. The protein encoded is a replication-independent member of the histone H3 family.
      Gene Symbol
      • H3F3A
      • MGC87783
      • H3.3A
      • MGC87782
      • H3F3
      • H3.3B
      • H3F3B
      Modifications
      • Methylation
      Purification MethodAntiserum
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q16695 # Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
      SIZE: 136 amino acids; 15508 Da
      SUBUNIT: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.
      SUBCELLULAR LOCATION: Nucleus.
      PTM: Acetylation is generally linked to gene activation. Acetylation on Lys-10 impairs methylation at Arg-9. Acetylation on Lys-19 and Lys-24 favors methylation at Arg-18 (By similarity). & Citrullination at Arg-9 and/or Arg-18 by PADI4 impairs methylation and represses transcription (By similarity). & Asymmetric dimethylation at Arg-18 by CARM1 is linked to gene activation. Symmetric dimethylation at Arg-9 by PRMT5 is linked to gene repression (By similarity). & Methylation at Lys-5, Lys-37 and Lys-80 are linked to gene activation. Methylation at Lys-5 facilitates subsequent acetylation of H3 and H4. Methylation at Lys-80 is associated with DNA double-strand break (DSB) responses and is a specific target for TP53BP1. Methylation at Lys-10 and Lys-28 are linked to gene repression. Methylation at Lys-10 is a specific target for HP1 proteins (CBX1, CBX3 and CBX5) and prevents subsequent phosphorylation at Ser-11 and acetylation of H3 and H4. Methylation at Lys-5 and Lys-80 require preliminary monoubiquitination of H2B at 'Lys-120'. Methylation at Lys-10 and Lys-28 are enriched in inactive X chromosome chromatin (By similarity). & Phosphorylated at Thr-4 by GSG2/haspin during prophase and dephosphorylated during anaphase. At centromeres, specifically phosphorylated at Thr-12 from prophase to early anaphase. Phosphorylated at Ser-11 during the whole mitosis. Phosphorylation at Ser-11, which is linked to gene activation, prevents methylation at Lys-10 but facilitates acetylation of H3 and H4. Phosphorylated at Ser-29 by MLTK isoform 1, RPS6KA5 or AURKB during mitosis or upon ultraviolet B irradiation (By similarity). & Phosphorylation at 'Ser-11' is crucial for chromosome condensation and cell-cycle progression during mitosis and meiosis. In addition phosphorylation at 'Ser-11' is important during interphase because it enables the transcription of genes following external stimulation, like stress or growth factors. Phosphorylation at 'Ser-11' is also an essential regulatory mechanism for neoplastic cell transformation. Phosphorylation at 'Ser-11' by AURKB/Aurora-B mediates the dissociation of HP1 proteins (CBX1, CBX3 and CBX5) from heterochromatin. & Ubiquitinated (By similarity).
      SIMILARITY: SwissProt: Q16695 ## Belongs to the histone H3 family.
      Molecular Weight17kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by immunoblot in chicken core histones
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions2 years at -20°C
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-monomethyl-Histone H3 (Lys36) Antibody SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-monomethyl-Histone H3 (Lys36) Antibody Certificates of Analysis

      TitleLot Number
      Anti-monomethyl-Histone H3 (Lys36) - 23165452316545
      Anti-monomethyl-Histone H3 (Lys36) - 2679526795
      Anti-monomethyl-Histone H3 (Lys36) -25669332566933
      Anti-monomethyl-Histone H3 (Lys36) -25864552586455
      Anti-monomethyl-Histone H3 (Lys36) -28345372834537
      Anti-monomethyl-Histone H3 (Lys36) Polyclonal Antibody3035136
      Anti-monomethyl-Histone H3 (Lys36) Polyclonal Antibody2900700

      References

      Reference overviewPub Med ID
      Intracellular α-ketoglutarate maintains the pluripotency of embryonic stem cells.
      Carey, BW; Finley, LW; Cross, JR; Allis, CD; Thompson, CB
      Nature  518  413-6  2015

      Show Abstract
      25487152 25487152
      Autocatalytic differentiation of epigenetic modifications within the Arabidopsis genome.
      Inagaki, S; Miura-Kamio, A; Nakamura, Y; Lu, F; Cui, X; Cao, X; Kimura, H; Saze, H; Kakutani, T
      The EMBO journal  29  3496-506  2010

      Show Abstract
      20834229 20834229
      The target of the NSD family of histone lysine methyltransferases depends on the nature of the substrate.
      Li, Yan, et al.
      J. Biol. Chem., 284: 34283-95 (2009)  2009

      Show Abstract
      19808676 19808676

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      Life Science Research > Antibodies and Assays > Primary Antibodies