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MABN540 | Anti-mGluR5 Antibody, clone N75/33

100 µg  
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      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      R, H, M WB, IHC M Purified Monoclonal Antibody
      Catalogue NumberMABN540
      DescriptionAnti-mGluR5 Antibody, clone N75/33
      Alternate Names
      • Metabotropic glutamate receptor 5
      • mGluR5
      Background InformationMetabotropic glutamate receptor 5 (mGluR5) is a G-protein mediated glutamate receptor that generates a calcium-activated chloride current. mGluR5 is widely distributed in neuronal cells of the central nervous system.
      Product Information
      • Hypothalamus tissue lysate
      PresentationPurified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      ApplicationDetect mGluR5 using this mouse monoclonal antibody, Anti-mGluR5 Antibody, clone N75/33 validated for use in western blotting & IHC.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      Application NotesWestern Blotting Analysis: 1 µg/mL from a representative lot detected mGluR5 in 10 µg of rat brain membrane tissue.

      Immunohistochemistry Analysis: A 1:100 dilution from a representative lot detected mGluR5 in normal human midbrain tissue.
      Biological Information
      ImmunogenRecombinant protein corresponding to the C-terminus of rat mGluR5.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      SpecificityThis antibody recognizes the C-terminus of mGluR5. This antibody may cross react with mGluR1 based upon <35% sequence homology.
      Species Reactivity
      • Rat
      • Human
      • Mouse
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Grm5
      • Gprc1e
      • Mglur5
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~150-270 kDa observed. This protein may be observed at higher molecular weights due to glycosylation (Bhave, G., et al. (2003). J Biol Chem. 278(32):30294-30301). This protein is observed as a dimer at ~270 kDa, but is observed as a monomer at ~140 kDa under reduced conditions in SDS-PAGE (Romano, C., et al. (1996). J Biol Chem. 271(45):28612-28616.). Uncharacterized band(s) may be observed in some cell lysates.
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in mouse hypothalamus tissue lysate.

      Western Blotting Analysis: 1 µg/mL of this antibody detected mGluR5 in 10 µg of mouse hypothalamus tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information