ABN26 Anti-iNOS/NOS II Antibody, NT

ABN26
100 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, H WB, IP, IH(P) Rb Affinity Purified Polyclonal Antibody
      Description
      Catalogue NumberABN26
      Replaces06-573
      DescriptionAnti-iNOS/NOS II Antibody, NT
      Alternate Names
      • Nitric oxide synthase, inducible
      • Inducible NO synthase
      • Inducible NOS
      • iNOS
      • Macrophage NOS
      • MAC-NOS
      • NOS type II
      • Peptidyl-cysteine S-nitrosylase NOS2
      Background InformationNitric oxide (NO) is an inorganic, gaseous free radical that carries a variety of messages between cells. Vasorelaxation, neurotransmission and cytotoxicity can all be potentiated through cellular response to NO. NO production is mediated by members of the nitric oxide synthase (NOS) family. NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO. Two constitutive isoforms, brain or neuronal NOS (b or nNOS, type I) & endothelial cell NOS (eNOS, type III), and one inducible isoform (iNOS, type II), have been cloned. Cytokines such as interferon-gamma (IFN), tumor necrosis factor (TNF), interleukin-1 and -2, and lipopolysaccarides (LPS) cause an increase in iNOS mRNA, protein, and activity levels. Protein kinase C-stimulating agents exhibit the same effect on iNOS activity. Human iNOS is regulated by calcium/calmodulin (in contrast with mouse NOS2).
      References
      Product Information
      FormatAffinity Purified
      Control
      • IFNgamma/LPS untreated and treated RAW264.7 cell lysates
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationDetect iNOS/NOS II using this Anti-iNOS/NOS II Antibody, NT validated for use in WB, IP, IH(P).
      Key Applications
      • Western Blotting
      • Immunoprecipitation
      • Immunohistochemistry (Paraffin)
      Application NotesImmunohistochemistry Analysis: 1:50-200 dilution from a representative lot detected iNOS/NOS II in malignant human lung tissues.

      Immunoprecipitation Analysis: 10 µg of this antibody immunoprecipitated iNOS/NOSII from IFNgamma/LPS treated RAW264.7 cell lysate.
      Biological Information
      ImmunogenGST-tagged recombinant protein corresponding to the N-terminus of mouse iNOS/NOS II.
      EpitopeN-terminus
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityThis antibody recognizes iNOS/NOS II at the N-terminus.
      Species Reactivity
      • Mouse
      • Human
      Species Reactivity NoteDemonstrated to react with Mouse and Human. Other homologies: Rat (85% sequence homology).
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Nos2
      • Inosl
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFUNCTION: Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such COX2.

      CATALYTIC ACTIVITY: L-arginine + n NADPH + n H+ + m O2 = citrulline + nitric oxide + n NADP+.

      COFACTOR: Heme group.

      Binds 1 FAD.

      Binds 1 FMN.

      Tetrahydrobiopterin (BH4). May stabilize the dimeric form of the enzyme.

      ENZYME REGULATION: Not stimulated by calcium/calmodulin. Aspirin inhibits expression and function of this enzyme and effects may be exerted at the level of translational/post-translational modification and directly on the catalytic activity.

      SUBUNIT STRUCTURE: Homodimer. Binds SLC9A3R1 (By similarity).

      TISSUE SPECIFICTY: Macrophages.

      INDUCTION: By treatment with endotoxins or cytokines.

      SEQUENCE SIMILARITIES: Belongs to the NOS family.

      Contains 1 FAD-binding FR-type domain.

      Contains 1 flavodoxin-like domain.
      Molecular Weight~125 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in IFNgamma/LPS untreated and treated RAW264.7 cell lysates.

      Western Blot Analysis: 0.5 µg/mL of this antibody detected iNOS/NOS II on 10 µg of IFNgamma/LPS untreated and treated RAW264.7 cell lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-iNOS/NOS II, NT2445053
      Anti-iNOS/NOS II, NT (rabbit polyclonal) Polyclonal AntibodyQ2922275
      Anti-iNOS/NOS II, NT - 21189722118972
      Anti-iNOS/NOS II, NT - 21530502153050
      Anti-iNOS/NOS II, NT - 23772982377298
      Anti-iNOS/NOS II, NT - 24247982424798
      Anti-iNOS/NOS II, NT - 22244932224493
      Anti-iNOS/NOS II, NT - 22817002281700
      Anti-iNOS/NOS II, NT - 29823802982380
      Anti-iNOS/NOS II, NT - NG1904639NG1904639

      References

      Reference overviewApplicationPub Med ID
      Crosstalk between glioma-initiating cells and endothelial cells drives tumor progression.
      Jeon, HM; Kim, SH; Jin, X; Park, JB; Kim, SH; Joshi, K; Nakano, I; Kim, H
      Cancer research  74  4482-92  2014

      Show Abstract
      Immunofluorescence24962027 24962027
      SMAD3 deficiency promotes inflammatory aortic aneurysms in angiotensin II-infused mice via activation of iNOS.
      Tan, CK; Tan, EH; Luo, B; Huang, CL; Loo, JS; Choong, C; Tan, NS
      Journal of the American Heart Association  2  e000269  2013

      Show Abstract
      23782924 23782924

      Brochure

      Title
      Pathways and Biomarkers of Oxidative Stress (EMD)

      Technical Info

      Title
      White Paper- Modern Methods in Oxidative Stress Research (EMD)

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies