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ABE1931 | Anti-hnRNP U/SAF-A Antibody

ABE1931
100 μg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M WB, IP, ICC Rb Affinity Purified Polyclonal Antibody
      Description
      Catalogue NumberABE1931
      DescriptionAnti-hnRNP U/SAF-A Antibody
      Alternate Names
      • Heterogeneous nuclear ribonucleoprotein U
      • hnRNP U/SAF-A
      • hnRNP U
      • Scaffold attachment factor A
      • SAF-A
      Background InformationHeterogeneous nuclear ribonucleoprotein U (UniProt Q8VEK3; also known as hnRNP U, Nuclear matrix protein sp120, SAF-A, Scaffold attachment factor A) is encoded by the Hnrnpu (also known as Hnrpu, SAFA, Sp120) gene (Gene ID 51810) in murine species. The scaffold attachment factor A (SAF-A) is a component of heterogeneous ribonucleoprotein (hnRNP) particles. About 50% of the protein is tightly associated with nuclear matrix. The N-terminal region of hnRNP U/SAF-A interacts with A/T-rich areas of double-stranded DNA that is also known as the scaffold attachment regions. SAF-A is also known to use its C-terminal RGG domain for targeting G/U-rich heterogeneous RNA. The ability of hnRNP U/SAF-A to bind to both DNA and RNA implicates a critical role of SAF-A in diverse nuclear functions, including transcriptional regulation, telomere length control, and X chromosome inactivation (XCI).
      References
      Product Information
      FormatAffinity Purified
      PresentationPurified rabbit polyclonal antibody in Tris-HCl 50 mM NaCl with 0.05% sodium azide and 10% glycerol.
      Applications
      ApplicationThis Anti-hnRNP U/SAF-A Antibody is validated for use in Western Blotting, Immunoprecipitation, Immunocytochemistry for the detection of hnRNP U/SAF-A .
      Key Applications
      • Western Blotting
      • Immunoprecipitation
      • Immunocytochemistry
      Application NotesImmunoprecipitation Analysis: A representative lot immunprecipitated hnRNP U/SAF-A using the nuclear extracts from a male mouse embryonic stem cell line P6D7 that bears a doxycycline-inducible Xist transgene on chromosome 17 (Courtesy of Dr. Neil Brockdorff, University of Oxford, UK).
      Immunoprecipitation/Mass Analysis: Target specificity of a representative lot was confirmed by MASS spectroscopy (MS) analysis of immunoprecipitates obtained from the nuclear extract of a male mouse embryonic stem cell (ESC) line P6D7 (Courtesy of Dr. Neil Brockdorff, University of Oxford, UK).
      Immunocytochemistry Analysis: A representative lot detected nuclear hnRNP U/SAF-A immunoreactivity by fluorescence immunocytochemistry using cells isolated from female mus spretus (Algerian mice) ear (FSPE) tissue, male mouse embryonic stem cells (3E ESCs), female mouse trophoblast stem cells (B1 TSCs), and a female mouse-derived cell line Neuro-2a/N2A (Courtesy of Dr. Neil Brockdorff, University of Oxford, UK).
      Immunocytochemistry Analysis: A representative lot was used in combination with fluorescent in situ hybridization (Immuno-FISH) to reveal the spatial association of Xist RNA with the nuclear matrix in the Barr body of individual C2C12 murine myoblasts by super-resolution three-dimensional structured illumination microscopy (3D-SIM) (Smeeet, D., et al. (2014). Epigenetics Chromatin. 7:8).
      Western Blotting Analysis: Prior to the production of Cat. No. ABE1931 by affinity purification, the antiserum immunoreactivity was confirmed by its detection of endogenous hnRNP U/SAF-A using HEK293T nuclear extract, as well as whole cell lysates from female mouse-derived PGK12.1 ESCs and female mus spretus (Algerian mice) ear (FSPE) tissue-derived cells (Courtesy of Dr. Neil Brockdorff, University of Oxford, UK).
      Biological Information
      ImmunogenRecombinant protein corresponding to the N-terminal of mouse hnRNP U/SAF-A.
      EpitopeN-terminal
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityReacts with murine and human hnRNP U/SAF-A. Expected to react with both the Long and Short spliced isoforms of human hnRNP U/SAF-A.
      Species Reactivity
      • Human
      • Mouse
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Hnrnpu
      • Hnrpu
      Purification MethodAffinity Purfied
      UniProt Number
      Molecular Weight~120 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HEK293 nuclear extract.

      Western Blotting Analysis: 0.5 µg/mL of this antibody detected hnRNP U/SAF-A in 10 µg of HEK293 nuclear extract.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Certificates of Analysis

      TitleLot Number
      Anti-hnRNP U/SAF-A -Q2623738 Q2623738

      References

      Reference overviewPub Med ID
      Three-dimensional super-resolution microscopy of the inactive X chromosome territory reveals a collapse of its active nuclear compartment harboring distinct Xist RNA foci.
      Smeets, D; Markaki, Y; Schmid, VJ; Kraus, F; Tattermusch, A; Cerase, A; Sterr, M; Fiedler, S; Demmerle, J; Popken, J; Leonhardt, H; Brockdorff, N; Cremer, T; Schermelleh, L; Cremer, M
      Epigenetics & chromatin 7 8 2014

      Show Abstract
      25057298 25057298