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PC388 | Anti-hMre-11 Rabbit pAb

PC388
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      H, M, R Rb Polyclonal Antibody

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      PC388-100UG
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      Limited AvailabilityLimited Availability
      Stocked 
      Discontinued
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      Available
        Remaining : Will advise
          Remaining : Will advise
          Will advise
          Contact Customer Service

          Glass bottle 100 μg
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          Description
          OverviewRecognizes the ~85 kDa hMre-11 protein in IMR90 and K562 cells.
          Catalogue NumberPC388
          Brand Family Calbiochem®
          Application Data
          Detection of human hMre11 by immunocytochemistry. Sample: IMR90 primary human fibroblasts left untreated (panel A) or treated with ionizing radiation (panel B). Primary antibody: Anti-hMre-11 Rabbit pAb (Cat. No. PC388T) (1 µg/ml). Detection: fluorescence. Courtesy of R. Maser and J. Petrini.
          References
          ReferencesDong, Z., et al. 1999. J. Biol. Chem. 274, 19513.
          Luo, G., et al. 1999. Proc. Natl. Acad. Sci. USA 96, 7376.
          Petrini, J.H. 1999. Am. J. Hum. Genet. 64, 1264.
          Carney, J.P., et al. 1998. Cell 93, 477.
          Haber, J.E. 1998. Cell 95, 583.
          Nelms, B.E., et al. 1998. Science 280, 590.
          Maser, R.S., et al. 1997. Mol. Cell Biol. 17, 6087.
          Xiao, Y. and D.T. Weaver. 1997. Nucleic Acids Res. 25, 2985.
          Dolganov, G.M., et al. 1996. Mol. Cell Biol. 16, 4832.
          Petrini, J.H., et al. 1995. Genomics 29, 80.
          Product Information
          FormLiquid
          FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.
          Positive controlIMR90 or K562 cells
          Preservative≤0.1% sodium azide
          Applications
          Key Applications Immunoblotting (Western Blotting)
          Immunofluorescence
          Immunoprecipitation
          Application NotesImmunoblotting (1 µg/ml)
          Immunofluorescence (1 µg/ml)
          Immunoprecipitation (1 µg/reaction)
          Application CommentsAntibody should be titrated for optimal results in individual systems.
          Biological Information
          Immunogenrecombinant, human hMre11 protein
          ImmunogenHuman
          HostRabbit
          IsotypeIgG
          Species Reactivity
          • Human
          • Mouse
          • Rat
          Antibody TypePolyclonal Antibody
          Concentration Label Please refer to vial label for lot-specific concentration
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Blue Ice Only
          Toxicity Standard Handling
          Storage +2°C to +8°C
          Do not freeze Yes
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          PC388

          References

          Reference overview
          Dong, Z., et al. 1999. J. Biol. Chem. 274, 19513.
          Luo, G., et al. 1999. Proc. Natl. Acad. Sci. USA 96, 7376.
          Petrini, J.H. 1999. Am. J. Hum. Genet. 64, 1264.
          Carney, J.P., et al. 1998. Cell 93, 477.
          Haber, J.E. 1998. Cell 95, 583.
          Nelms, B.E., et al. 1998. Science 280, 590.
          Maser, R.S., et al. 1997. Mol. Cell Biol. 17, 6087.
          Xiao, Y. and D.T. Weaver. 1997. Nucleic Acids Res. 25, 2985.
          Dolganov, G.M., et al. 1996. Mol. Cell Biol. 16, 4832.
          Petrini, J.H., et al. 1995. Genomics 29, 80.

          Brochure

          Title
          DNA Repair Pathway Poster
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision10-October-2007 RFH
          ApplicationImmunoblotting (1 µg/ml)
          Immunofluorescence (1 µg/ml)
          Immunoprecipitation (1 µg/reaction)
          Application Data
          Detection of human hMre11 by immunocytochemistry. Sample: IMR90 primary human fibroblasts left untreated (panel A) or treated with ionizing radiation (panel B). Primary antibody: Anti-hMre-11 Rabbit pAb (Cat. No. PC388T) (1 µg/ml). Detection: fluorescence. Courtesy of R. Maser and J. Petrini.
          DescriptionAffinity purified rabbit polyclonal antibody. Recognizes the ~85 kDa hMre-11 protein.
          BackgroundDouble strand breaks (DSB) in DNA are repaired by an evolutionarily conserved complex that includes the proteins Mre11 and Rad50. Both of these proteins are essential since nullizygous embryos die in utero, consistent with the fact that they both participate in two distinct processes: homologous recombination and non-homologous end joining. The Mre11 gene encodes a 680 amino acid protein that migrates on SDS/with an apparent mobility of ~85 kDa. Among many different functions ascribed to it, Mre11 exhibits a Mn2+-dependent 3'-5' exonuclease activity on double stranded DNA and a single-stranded endonuclease that facilitates repair of double strand breaks in DNA. Activation of Mre11 may involve post-translational modification since hyperphosphorylation is observed upon DNA damage. Efficient repair of DSBs in mammalian cells appears to require formation of nuclear foci comprised of Mre11, Rad50, and p95NBS1. These foci are observed following induction of DSBs with ionizing radiation, and ionizing radiation-induced foci (IRIF) containing Mre11 appear in a radiation dose dependent manner that slowly dissipates over time, presumably after DSB repair is completed. IRIF containing Mre11 do not form in cells lacking p95NBS1; and in cells lacking ATM, the number of IRIF is dramatically reduced. While Mre11's catalytic activites are essential for repair of DSBs, the Mre11/Rad50/p95NBS1 appears to function as a primary detector of DNA damage.
          HostRabbit
          Immunogen speciesHuman
          Immunogenrecombinant, human hMre11 protein
          IsotypeIgG
          Specieshuman, mouse, rat
          Positive controlIMR90 or K562 cells
          FormLiquid
          FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.
          Concentration Label Please refer to vial label for lot-specific concentration
          Preservative≤0.1% sodium azide
          CommentsAntibody should be titrated for optimal results in individual systems.
          Storage +2°C to +8°C
          Do Not Freeze Yes
          Toxicity Standard Handling
          ReferencesDong, Z., et al. 1999. J. Biol. Chem. 274, 19513.
          Luo, G., et al. 1999. Proc. Natl. Acad. Sci. USA 96, 7376.
          Petrini, J.H. 1999. Am. J. Hum. Genet. 64, 1264.
          Carney, J.P., et al. 1998. Cell 93, 477.
          Haber, J.E. 1998. Cell 95, 583.
          Nelms, B.E., et al. 1998. Science 280, 590.
          Maser, R.S., et al. 1997. Mol. Cell Biol. 17, 6087.
          Xiao, Y. and D.T. Weaver. 1997. Nucleic Acids Res. 25, 2985.
          Dolganov, G.M., et al. 1996. Mol. Cell Biol. 16, 4832.
          Petrini, J.H., et al. 1995. Genomics 29, 80.