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07-520 | Anti-eNOS/NOS III Antibody, CT

200 µg  
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      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, R, B, M, Ca, Gp, Po WB Rb Purified Polyclonal Antibody
      Catalogue Number07-520
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-eNOS/NOS III Antibody, CT
      OverviewNote: ADMA (Catalog #19-169) and NMMA (Catalog #19-170) are inhibitors of NOS
      Alternate Names
      • Constitutive NOS
      • Endothelial NOS
      • Endothelial NOS
      • Endothelial NOS
      • endothelial nitric oxide synthase
      • nitric oxide synthase 3 (endothelial cell)
      Background InformationEndothelial NOS (eNOS), also known as Nitric oxide synthase 3 (NOS3) or constitutive NOS (cNOS), is a nitric oxide synthase. It generates NO in blood vessels and is involved with regulating vascular function. A constitutive Ca2+ dependent NOS provides a basal release of NO. eNOS is associated with plasma membranes surrounding cells and the membranes of Golgi bodies within cells.
      Product Information
      • Platelets, placenta, liver and kidney.
      PresentationPurified rabbit polyclonal IgG in buffer containing 0.014 M phosphate buffer, pH 7.6, 0.175 M NaCl, 0.07% sodium azide before the addition of glycerol to 30%. Liquid at -20°C.
      ApplicationAnti-eNOS/NOS III Antibody, CT detects level of eNOS/NOS III & has been published & validated for use in WB.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenKLH-conjugated, synthetic peptide corresponding to amino acids 1181-1194 (C-QERQLRGAVPWAFD) of human eNOS/NOS III. The immunizing sequence has 10/11 identical amino acids in mouse, guinea pig, bovine, pig, rat, and dog.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      SpecificityRecognizes eNOS/NOS III, Mr ~135 kDa.
      Species Reactivity
      • Human
      • Rat
      • Bovine
      • Mouse
      • Canine
      • Guinea Pig
      • Pig
      Species Reactivity NoteHuman and bovine.
      Predicted to cross-react with mouse, guinea pig, pig, rat and dog based on sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • NOS3
      • cNOS
      • ECNOS
      • eNOS
      • EC-NOS
      • NOSIII
      Purification MethodProtein A Purfied
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P29474 # Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. No mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.
      COFACTOR: Heme group. & Binds 1 FAD. & Binds 1 FMN. & Metrahydrobiopterin (BH4). May stabilize the dimeric form of the enzyme.
      SIZE: 1203 amino acids; 133289 Da
      SUBUNIT: Homodimer. Interacts with NOSIP and NOSTRIN.
      SUBCELLULAR LOCATION: Cell membrane, caveola. Cytoplasm, cytoskeleton. Golgi apparatus. Note=Specifically associates with actin cytoskeleton in the G2 phase of the cell cycle; which is favored by interaction with NOSIP and results in a reduced enzymatic activity.
      TISSUE SPECIFICITY: Platelets, placenta, liver and kidney.
      SIMILARITY: SwissProt: P29474 ## Belongs to the NOS family. & Contains 1 flavodoxin-like domain. ... hide » see more »
      Molecular Weight135 kDa
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by western blot in RIPA lysates from HUVEC cells.

      Western Blot Analysis:
      0.5-2 µg/mL of this lot detected eNOS/NOS III in RIPA lysates from HUVEC cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      For maximum recovery of product, centrifuge the vial prior to removing the cap.
      Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
      Packaging Information
      Material Size200 µg
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-eNOS/NOS III, CT 2476971
      Anti-eNOS/NOS III, CT (rabbit polyclonal IgG) - DAM1424997 DAM1424997
      Anti-eNOS/NOS III, CT - 2203125 2203125
      Anti-eNOS/NOS III, CT - 2293831 2293831
      Anti-eNOS/NOS III, CT - 24650 24650
      Anti-eNOS/NOS III, CT - DAM1424997 DAM1424997
      Anti-eNOS/NOS III, CT -2503006 2503006
      Anti-eNOS/NOS III, CT -2610449 2610449
      Anti-eNOS/NOS III, CT -2686930 2686930
      Anti-eNOS/NOS III, CT -2708538 2708538


      Reference overviewSpeciesPub Med ID
      TJ0711, a novel vasodilatory β-blocker, protects SHR rats against hypertension induced renal injury.
      Yang, Juan, et al.
      Am J Transl Res, 5: 279-90 (2013) 2013

      Show Abstract
      23634239 23634239
      Apigenin inhibits the TNFα-induced expression of eNOS and MMP-9 via modulating Akt signalling through oestrogen receptor engagement.
      Daniela Palmieri,Patrizia Perego,Domenico Palombo
      Molecular and cellular biochemistry 371 2012

      Show Abstract
      22899172 22899172
      Fetal pulmonary vascular remodeling in a rat model induced by hypoxia and indomethacin.
      Xue-Feng Xu,Wei-Zhong Gu,Xi-Ling Wu,Ru-Yi Li,Li-Zhong Du
      The journal of maternal-fetal & neonatal medicine : the official journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians 24 2011

      Show Abstract
      20459333 20459333
      Functional protein network activation mapping reveals new potential molecular drug targets for poor prognosis pediatric BCP-ALL.
      Accordi, Benedetta, et al.
      PLoS ONE, 5: e13552 (2010) 2010

      Show Abstract
      21042412 21042412
      BDNF/TrkB content and interaction with gastrin-releasing peptide receptor blockade in colorectal cancer.
      Caroline Brunetto de Farias,Denis Broock Rosemberg,Tiago Elias Heinen,Patricia Koehler-Santos,Ana Lucia Abujamra,Flávio Kapczinski,Algemir Lunardi Brunetto,Patricia Ashton-Prolla,Luise Meurer,Maurício Reis Bogo,Daniel C Damin,Gilberto Schwartsmann,Rafael Roesler
      Oncology 79 2010

      Show Abstract
      21474968 21474968
      The senescence-accelerated mouse (SAM-P8) as a model for the study of vascular functional alterations during aging.
      Lloréns, Silvia, et al.
      Biogerontology, 8: 663-72 (2007) 2007

      Mouse17786580 17786580


      Pathways and Biomarkers of Oxidative Stress (EMD)

      Technical Info

      White Paper- Modern Methods in Oxidative Stress Research (EMD)

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