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07-367 | Anti-dimethyl-Histone H4 (Lys20) Antibody

07-367
200 µL  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      Vrt IF, WB, ICC Rb Serum Polyclonal Antibody
      Description
      Catalogue Number07-367
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-dimethyl-Histone H4 (Lys20) Antibody
      Alternate Names
      • H4K20me2
      • Histone H4 (di methyl K20)
      Background InformationHistone H4 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H4 is involved with the structure of the nucleosomes of the 'beads on a string' structure.

      The N-terminal tail of histone H4 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.
      References
      Product Information
      FormatSerum
      Control
      • HeLa cell acid extracts
      PresentationProtein A Purified immunoglobulin in 0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide and 30% glycerol.
      Applications
      ApplicationUse Anti-dimethyl-Histone H4 (Lys20) Antibody (Rabbit Polyclonal Antibody) validated in ChIP, IF, WB, ICC to detect dimethyl-Histone H4 (Lys20) also known as H4K20me2, Histone H4 (di methyl K20).
      Key Applications
      • Immunofluorescence
      • Western Blotting
      • Immunocytochemistry
      Biological Information
      Immunogenpeptide corresponding to amino acids 15-25 of human, mouse and rat Histone H4(AKRHRdimKVLRDN-C) dimethylated on lysine 20.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityRecognizes Histone H4 dimethylated on lysine 20.
      IsotypeIgG
      Species Reactivity
      • Vertebrates
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryHistones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6.
      Gene Symbol
      • HIST1H4A
      • H4/J
      • HIST1H4F
      • HIST1H4L
      • H4FN
      • H4FA
      • H4FH
      • HIST1H4H
      • H4F2
      • H4FM
      • H4/A
      • H4FK
      • H4FG
      • HIST2H4
      • H4FB
      • H4/K
      • HIST1H4I
      • H4/N
      • HIST1H4B
      • H4FE
      • H4/M
      • H4/a
      • HIST1H4E
      • HIST4H4
      • HIST2H4A
      • H4FC
      • H4FI
      • H4/H
      • HIST1H4C
      • H4/G
      • HIST1H4K
      • H4FJ
      • H4FD
      • H4/I
      • H4/B
      • H4/D
      • H4/C
      • HIST1H4J
      • HIST1H4D
      • H4/E
      Modifications
      • Methylation
      Purification MethodUnpurified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P62805 # Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
      SIZE: 103 amino acids; 11367 Da
      SUBUNIT: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.
      SUBCELLULAR LOCATION: Nucleus.
      PTM: Symmetric dimethylation on Arg-4 by the PRDM1/PRMT5 complex may play a crucial role in the germ-cell lineage (By similarity). & Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins.
      SIMILARITY: SwissProt: P62805 ## Belongs to the histone H4 family.
      Molecular Weight10 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by immunoblot in acid extracts from HeLa cells
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
      Packaging Information
      Material Size200 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-dimethyl-Histone H4 (Lys20) (rabbit antiserum) - 2113357 2113357
      Anti-dimethyl-Histone H4 (Lys20) (rabbit antiserum) - 2188670 2188670
      Anti-dimethyl-Histone H4 (Lys20) (rabbit antiserum) - 2201576 2201576
      Anti-dimethyl-Histone H4 (Lys20) (rabbit antiserum) - 2322515 2322515
      Anti-dimethyl-Histone H4 (Lys20) - 22433 22433
      Anti-dimethyl-Histone H4 (Lys20) - 2517831 2517831
      Anti-dimethyl-Histone H4 (Lys20) - 27524 27524
      Anti-dimethyl-Histone H4 (Lys20) - 32204 32204
      Anti-dimethyl-Histone H4 (Lys20) - DAM1543701 DAM1543701
      Anti-dimethyl-Histone H4 (Lys20) - DAM1661074 DAM1661074
      Anti-dimethyl-Histone H4 (Lys20) - DAM1705073 DAM1705073
      Anti-dimethyl-Histone H4 (Lys20) - DAM1745325 DAM1745325

      References | 16 Available | See All References

      Reference overviewApplicationPub Med ID
      Radiation-induced alterations of histone post-translational modification levels in lymphoblastoid cell lines.
      Maroschik, B; Gürtler, A; Krämer, A; Rößler, U; Gomolka, M; Hornhardt, S; Mörtl, S; Friedl, AA
      Radiation oncology (London, England) 9 15 2014

      Show Abstract
      Western Blotting24406105 24406105
      Cerebellar oxidative DNA damage and altered DNA methylation in the BTBR T+tf/J mouse model of autism and similarities with human post mortem cerebellum.
      Shpyleva, S; Ivanovsky, S; de Conti, A; Melnyk, S; Tryndyak, V; Beland, FA; James, SJ; Pogribny, IP
      PloS one 9 e113712 2014

      Show Abstract
      Western Blotting25423485 25423485
      Caenorhabditis elegans dosage compensation regulates histone H4 chromatin state on X chromosomes.
      Wells, MB; Snyder, MJ; Custer, LM; Csankovszki, G
      Molecular and cellular biology 32 1710-9 2012

      Show Abstract
      Immunofluorescence22393255 22393255
      Ornithine decarboxylase antizyme induces hypomethylation of genome DNA and histone H3 lysine 9 dimethylation (H3K9me2) in human oral cancer cell line.
      Yamamoto, D; Shima, K; Matsuo, K; Nishioka, T; Chen, CY; Hu, GF; Sasaki, A; Tsuji, T
      PloS one 5 e12554 2010

      Show Abstract Full Text Article
      Western Blotting20838441 20838441
      Drosophila RB proteins repress differentiation-specific genes via two different mechanisms.
      Lee, H; Ohno, K; Voskoboynik, Y; Ragusano, L; Martinez, A; Dimova, DK
      Molecular and cellular biology 30 2563-77 2010

      Show Abstract Full Text Article
      20176807 20176807
      The target of the NSD family of histone lysine methyltransferases depends on the nature of the substrate.
      Li, Yan, et al.
      J. Biol. Chem., 284: 34283-95 (2009) 2009

      Show Abstract
      19808676 19808676
      Global repression of cancer gene expression in a zebrafish model of melanoma is linked to epigenetic regulation.
      Viviana Anelli,Cristina Santoriello,Martin Distel,Reinhard W Köster,Francesca D Ciccarelli,Marina Mione
      Zebrafish 6 2009

      Show Abstract
      20047469 20047469
      Ordered histone modifications are associated with transcriptional poising and activation of the phaseolin promoter.
      Ng, DW; Chandrasekharan, MB; Hall, TC
      The Plant cell 18 119-32 2006

      Show Abstract Full Text Article
      16326929 16326929
      Partitioning of the maize epigenome by the number of methyl groups on histone H3 lysines 9 and 27.
      Shi, J; Dawe, RK
      Genetics 173 1571-83 2006

      Show Abstract Full Text Article
      Western Blotting, Immunofluorescence16624902 16624902
      Profile of histone lysine methylation across transcribed mammalian chromatin.
      Vakoc, CR; Sachdeva, MM; Wang, H; Blobel, GA
      Molecular and cellular biology 26 9185-95 2006

      Show Abstract
      17030614 17030614

      Posters

      Title
      Histone Modifications